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Plumbagin Mediates Cardioprotection Against Myocardial Ischemia/Reperfusion Injury Through Nrf-2 Signaling

BACKGROUND: Plumbagin is a potent antioxidant with anti-inflammatory and anti-carcinogenic action. Myocardial ischemia/reperfusion injury results in organ damage through oxidative stress and inflammatory mechanisms. In this study, we analyzed the potential role of plumbagin against myocardial I/R in...

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Autores principales: Wang, Shi-Xun, Wang, Jian, Shao, Jing-Bo, Tang, Wei-ning, Zhong, Jing-Quan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835155/
https://www.ncbi.nlm.nih.gov/pubmed/27078001
http://dx.doi.org/10.12659/MSM.897618
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author Wang, Shi-Xun
Wang, Jian
Shao, Jing-Bo
Tang, Wei-ning
Zhong, Jing-Quan
author_facet Wang, Shi-Xun
Wang, Jian
Shao, Jing-Bo
Tang, Wei-ning
Zhong, Jing-Quan
author_sort Wang, Shi-Xun
collection PubMed
description BACKGROUND: Plumbagin is a potent antioxidant with anti-inflammatory and anti-carcinogenic action. Myocardial ischemia/reperfusion injury results in organ damage through oxidative stress and inflammatory mechanisms. In this study, we analyzed the potential role of plumbagin against myocardial I/R injury in Wistar rats. MATERIAL/METHODS: Oxidative stress was measured through ROS, lipid peroxide content, and antioxidant enzyme activities. The expression of redox signaling and inflammatory proteins was analyzed through Western blotting. Inflammatory cytokine expressions were determined through ELISA. RESULTS: Oxidative stress status was reduced by plumbagin by decreasing ROS and lipid peroxide levels in rats with myocardial I/R (MI/R) injury. Plumbagin regulated redox imbalance induced by I/R injury by modulating the transcription factors NF-κB and Nrf-2. Further, downstream targets of NF-κB (COX-2, iNOS) and Nrf-2 (HO-1, NQO1 and GST) expression were significantly downregulated by plumbagin treatment. Pro-inflammatory cytokine expressions were significantly abrogated by plumbagin treatment. CONCLUSIONS: This study shows the protective role of plumbagin against myocardial I/R injury by regulating antioxidant and inflammatory mechanisms.
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spelling pubmed-48351552016-05-02 Plumbagin Mediates Cardioprotection Against Myocardial Ischemia/Reperfusion Injury Through Nrf-2 Signaling Wang, Shi-Xun Wang, Jian Shao, Jing-Bo Tang, Wei-ning Zhong, Jing-Quan Med Sci Monit Animal Study BACKGROUND: Plumbagin is a potent antioxidant with anti-inflammatory and anti-carcinogenic action. Myocardial ischemia/reperfusion injury results in organ damage through oxidative stress and inflammatory mechanisms. In this study, we analyzed the potential role of plumbagin against myocardial I/R injury in Wistar rats. MATERIAL/METHODS: Oxidative stress was measured through ROS, lipid peroxide content, and antioxidant enzyme activities. The expression of redox signaling and inflammatory proteins was analyzed through Western blotting. Inflammatory cytokine expressions were determined through ELISA. RESULTS: Oxidative stress status was reduced by plumbagin by decreasing ROS and lipid peroxide levels in rats with myocardial I/R (MI/R) injury. Plumbagin regulated redox imbalance induced by I/R injury by modulating the transcription factors NF-κB and Nrf-2. Further, downstream targets of NF-κB (COX-2, iNOS) and Nrf-2 (HO-1, NQO1 and GST) expression were significantly downregulated by plumbagin treatment. Pro-inflammatory cytokine expressions were significantly abrogated by plumbagin treatment. CONCLUSIONS: This study shows the protective role of plumbagin against myocardial I/R injury by regulating antioxidant and inflammatory mechanisms. International Scientific Literature, Inc. 2016-04-14 /pmc/articles/PMC4835155/ /pubmed/27078001 http://dx.doi.org/10.12659/MSM.897618 Text en © Med Sci Monit, 2016 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License
spellingShingle Animal Study
Wang, Shi-Xun
Wang, Jian
Shao, Jing-Bo
Tang, Wei-ning
Zhong, Jing-Quan
Plumbagin Mediates Cardioprotection Against Myocardial Ischemia/Reperfusion Injury Through Nrf-2 Signaling
title Plumbagin Mediates Cardioprotection Against Myocardial Ischemia/Reperfusion Injury Through Nrf-2 Signaling
title_full Plumbagin Mediates Cardioprotection Against Myocardial Ischemia/Reperfusion Injury Through Nrf-2 Signaling
title_fullStr Plumbagin Mediates Cardioprotection Against Myocardial Ischemia/Reperfusion Injury Through Nrf-2 Signaling
title_full_unstemmed Plumbagin Mediates Cardioprotection Against Myocardial Ischemia/Reperfusion Injury Through Nrf-2 Signaling
title_short Plumbagin Mediates Cardioprotection Against Myocardial Ischemia/Reperfusion Injury Through Nrf-2 Signaling
title_sort plumbagin mediates cardioprotection against myocardial ischemia/reperfusion injury through nrf-2 signaling
topic Animal Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835155/
https://www.ncbi.nlm.nih.gov/pubmed/27078001
http://dx.doi.org/10.12659/MSM.897618
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