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Genetic Diversity of Dihydropteroate synthetase Gene (dhps) of Plasmodium vivax in Hormozgan Province, Iran

BACKGROUND: The present study was formulated in order to determine polymorphism of dihydropteroate synthetase gene (dhps) of Plasmodium vivax (P. vivax) in Hormozgan Province, southern Iran and mutations at codons 382, 383, 512, 553, and 585 associated with resistance of P. vivax to sulfadoxine. MET...

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Autores principales: MAGHSOODLOORAD, Somayeh, HAGHIGHI, Ali, SHARIFI SARASIABI, Khojasteh, TAGHIPOOR, Niloofar, HOSSEINZADEH, Nahid, GACHKAR, Latif, NAZEMALHOSSEINI MOJARRAD, Ehsan, MAGHSOODLOORAD, Elham
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835476/
https://www.ncbi.nlm.nih.gov/pubmed/27095975
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author MAGHSOODLOORAD, Somayeh
HAGHIGHI, Ali
SHARIFI SARASIABI, Khojasteh
TAGHIPOOR, Niloofar
HOSSEINZADEH, Nahid
GACHKAR, Latif
NAZEMALHOSSEINI MOJARRAD, Ehsan
MAGHSOODLOORAD, Elham
author_facet MAGHSOODLOORAD, Somayeh
HAGHIGHI, Ali
SHARIFI SARASIABI, Khojasteh
TAGHIPOOR, Niloofar
HOSSEINZADEH, Nahid
GACHKAR, Latif
NAZEMALHOSSEINI MOJARRAD, Ehsan
MAGHSOODLOORAD, Elham
author_sort MAGHSOODLOORAD, Somayeh
collection PubMed
description BACKGROUND: The present study was formulated in order to determine polymorphism of dihydropteroate synthetase gene (dhps) of Plasmodium vivax (P. vivax) in Hormozgan Province, southern Iran and mutations at codons 382, 383, 512, 553, and 585 associated with resistance of P. vivax to sulfadoxine. METHOD: One-hundred eighteen isolates of P. vivax were prepared within 2007–2008 to determine dihydrofolate reductase-thymidylate synthase (dhfr-ts) gene. The isolates were determined in the study of genetic diversity of dihydropteroate synthetase gene (dhps) of P. vivax. The study was performed via PCR test and nucleotide sequencing. RESULTS: Of 118 blood samples infected by P. vivax, 46 and 72 samples belonged to Minab and Jask, respectively. No mutation was detected at 5 target codons. However, among these 118 samples, three isolates (2.54%) were found to have a mutation at the new codon 421. CONCLUSION: Since mutation was detected in dihydrofolate reductase (Pvdhfr) gene in the same samples but no mutation was found at five main codons of Pvdhps gene, it can be concluded that P. vivax, considering their mutations in Pvdhfr, is still susceptible to sulfadoxine and therefore, to fansidar in Hormozgan Province, Southern Iran.
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spelling pubmed-48354762016-04-19 Genetic Diversity of Dihydropteroate synthetase Gene (dhps) of Plasmodium vivax in Hormozgan Province, Iran MAGHSOODLOORAD, Somayeh HAGHIGHI, Ali SHARIFI SARASIABI, Khojasteh TAGHIPOOR, Niloofar HOSSEINZADEH, Nahid GACHKAR, Latif NAZEMALHOSSEINI MOJARRAD, Ehsan MAGHSOODLOORAD, Elham Iran J Parasitol Short Communication BACKGROUND: The present study was formulated in order to determine polymorphism of dihydropteroate synthetase gene (dhps) of Plasmodium vivax (P. vivax) in Hormozgan Province, southern Iran and mutations at codons 382, 383, 512, 553, and 585 associated with resistance of P. vivax to sulfadoxine. METHOD: One-hundred eighteen isolates of P. vivax were prepared within 2007–2008 to determine dihydrofolate reductase-thymidylate synthase (dhfr-ts) gene. The isolates were determined in the study of genetic diversity of dihydropteroate synthetase gene (dhps) of P. vivax. The study was performed via PCR test and nucleotide sequencing. RESULTS: Of 118 blood samples infected by P. vivax, 46 and 72 samples belonged to Minab and Jask, respectively. No mutation was detected at 5 target codons. However, among these 118 samples, three isolates (2.54%) were found to have a mutation at the new codon 421. CONCLUSION: Since mutation was detected in dihydrofolate reductase (Pvdhfr) gene in the same samples but no mutation was found at five main codons of Pvdhps gene, it can be concluded that P. vivax, considering their mutations in Pvdhfr, is still susceptible to sulfadoxine and therefore, to fansidar in Hormozgan Province, Southern Iran. Tehran University of Medical Sciences 2016 /pmc/articles/PMC4835476/ /pubmed/27095975 Text en Copyright© Iranian Society of Parasitology & Tehran University of Medical Sciences This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Short Communication
MAGHSOODLOORAD, Somayeh
HAGHIGHI, Ali
SHARIFI SARASIABI, Khojasteh
TAGHIPOOR, Niloofar
HOSSEINZADEH, Nahid
GACHKAR, Latif
NAZEMALHOSSEINI MOJARRAD, Ehsan
MAGHSOODLOORAD, Elham
Genetic Diversity of Dihydropteroate synthetase Gene (dhps) of Plasmodium vivax in Hormozgan Province, Iran
title Genetic Diversity of Dihydropteroate synthetase Gene (dhps) of Plasmodium vivax in Hormozgan Province, Iran
title_full Genetic Diversity of Dihydropteroate synthetase Gene (dhps) of Plasmodium vivax in Hormozgan Province, Iran
title_fullStr Genetic Diversity of Dihydropteroate synthetase Gene (dhps) of Plasmodium vivax in Hormozgan Province, Iran
title_full_unstemmed Genetic Diversity of Dihydropteroate synthetase Gene (dhps) of Plasmodium vivax in Hormozgan Province, Iran
title_short Genetic Diversity of Dihydropteroate synthetase Gene (dhps) of Plasmodium vivax in Hormozgan Province, Iran
title_sort genetic diversity of dihydropteroate synthetase gene (dhps) of plasmodium vivax in hormozgan province, iran
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835476/
https://www.ncbi.nlm.nih.gov/pubmed/27095975
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