A High-Density Genetic Linkage Map for Cucumber (Cucumis sativus L.): Based on Specific Length Amplified Fragment (SLAF) Sequencing and QTL Analysis of Fruit Traits in Cucumber

High-density genetic linkage map plays an important role in genome assembly and quantitative trait loci (QTL) fine mapping. Since the coming of next-generation sequencing, makes the structure of high-density linkage maps much more convenient and practical, which simplifies SNP discovery and high-thr...

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Autores principales: Zhu, Wen-Ying, Huang, Long, Chen, Long, Yang, Jian-Tao, Wu, Jia-Ni, Qu, Mei-Ling, Yao, Dan-Qing, Guo, Chun-Li, Lian, Hong-Li, He, Huan-Le, Pan, Jun-Song, Cai, Run
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835494/
https://www.ncbi.nlm.nih.gov/pubmed/27148281
http://dx.doi.org/10.3389/fpls.2016.00437
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author Zhu, Wen-Ying
Huang, Long
Chen, Long
Yang, Jian-Tao
Wu, Jia-Ni
Qu, Mei-Ling
Yao, Dan-Qing
Guo, Chun-Li
Lian, Hong-Li
He, Huan-Le
Pan, Jun-Song
Cai, Run
author_facet Zhu, Wen-Ying
Huang, Long
Chen, Long
Yang, Jian-Tao
Wu, Jia-Ni
Qu, Mei-Ling
Yao, Dan-Qing
Guo, Chun-Li
Lian, Hong-Li
He, Huan-Le
Pan, Jun-Song
Cai, Run
author_sort Zhu, Wen-Ying
collection PubMed
description High-density genetic linkage map plays an important role in genome assembly and quantitative trait loci (QTL) fine mapping. Since the coming of next-generation sequencing, makes the structure of high-density linkage maps much more convenient and practical, which simplifies SNP discovery and high-throughput genotyping. In this research, a high-density linkage map of cucumber was structured using specific length amplified fragment sequencing, using 153 F(2) populations of S1000 × S1002. The high-density genetic map composed 3,057 SLAFs, including 4,475 SNP markers on seven chromosomes, and spanned 1061.19 cM. The average genetic distance is 0.35 cM. Based on this high-density genome map, QTL analysis was performed on two cucumber fruit traits, fruit length and fruit diameter. There are 15 QTLs for the two fruit traits were detected.
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spelling pubmed-48354942016-05-04 A High-Density Genetic Linkage Map for Cucumber (Cucumis sativus L.): Based on Specific Length Amplified Fragment (SLAF) Sequencing and QTL Analysis of Fruit Traits in Cucumber Zhu, Wen-Ying Huang, Long Chen, Long Yang, Jian-Tao Wu, Jia-Ni Qu, Mei-Ling Yao, Dan-Qing Guo, Chun-Li Lian, Hong-Li He, Huan-Le Pan, Jun-Song Cai, Run Front Plant Sci Plant Science High-density genetic linkage map plays an important role in genome assembly and quantitative trait loci (QTL) fine mapping. Since the coming of next-generation sequencing, makes the structure of high-density linkage maps much more convenient and practical, which simplifies SNP discovery and high-throughput genotyping. In this research, a high-density linkage map of cucumber was structured using specific length amplified fragment sequencing, using 153 F(2) populations of S1000 × S1002. The high-density genetic map composed 3,057 SLAFs, including 4,475 SNP markers on seven chromosomes, and spanned 1061.19 cM. The average genetic distance is 0.35 cM. Based on this high-density genome map, QTL analysis was performed on two cucumber fruit traits, fruit length and fruit diameter. There are 15 QTLs for the two fruit traits were detected. Frontiers Media S.A. 2016-04-19 /pmc/articles/PMC4835494/ /pubmed/27148281 http://dx.doi.org/10.3389/fpls.2016.00437 Text en Copyright © 2016 Zhu, Huang, Chen, Yang, Wu, Qu, Yao, Guo, Lian, He, Pan and Cai. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhu, Wen-Ying
Huang, Long
Chen, Long
Yang, Jian-Tao
Wu, Jia-Ni
Qu, Mei-Ling
Yao, Dan-Qing
Guo, Chun-Li
Lian, Hong-Li
He, Huan-Le
Pan, Jun-Song
Cai, Run
A High-Density Genetic Linkage Map for Cucumber (Cucumis sativus L.): Based on Specific Length Amplified Fragment (SLAF) Sequencing and QTL Analysis of Fruit Traits in Cucumber
title A High-Density Genetic Linkage Map for Cucumber (Cucumis sativus L.): Based on Specific Length Amplified Fragment (SLAF) Sequencing and QTL Analysis of Fruit Traits in Cucumber
title_full A High-Density Genetic Linkage Map for Cucumber (Cucumis sativus L.): Based on Specific Length Amplified Fragment (SLAF) Sequencing and QTL Analysis of Fruit Traits in Cucumber
title_fullStr A High-Density Genetic Linkage Map for Cucumber (Cucumis sativus L.): Based on Specific Length Amplified Fragment (SLAF) Sequencing and QTL Analysis of Fruit Traits in Cucumber
title_full_unstemmed A High-Density Genetic Linkage Map for Cucumber (Cucumis sativus L.): Based on Specific Length Amplified Fragment (SLAF) Sequencing and QTL Analysis of Fruit Traits in Cucumber
title_short A High-Density Genetic Linkage Map for Cucumber (Cucumis sativus L.): Based on Specific Length Amplified Fragment (SLAF) Sequencing and QTL Analysis of Fruit Traits in Cucumber
title_sort high-density genetic linkage map for cucumber (cucumis sativus l.): based on specific length amplified fragment (slaf) sequencing and qtl analysis of fruit traits in cucumber
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835494/
https://www.ncbi.nlm.nih.gov/pubmed/27148281
http://dx.doi.org/10.3389/fpls.2016.00437
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