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Phenotypic and Molecular Assessment of Drug Resistance Profile and Genetic Diversity of Waterborne Escherichia coli
Bacterial Escherichia coli isolates were derived from waters of the Nowohucki Reservoir (Cracow, Poland) in summer and winter seasons. In total, 94 strains, identified as E. coli, were isolated from five sampling sites in the area of the reservoir. Based on the disk-diffusion tests, it was found tha...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835524/ https://www.ncbi.nlm.nih.gov/pubmed/27158170 http://dx.doi.org/10.1007/s11270-016-2833-z |
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author | Wolny-Koładka, Katarzyna Lenart-Boroń, Anna |
author_facet | Wolny-Koładka, Katarzyna Lenart-Boroń, Anna |
author_sort | Wolny-Koładka, Katarzyna |
collection | PubMed |
description | Bacterial Escherichia coli isolates were derived from waters of the Nowohucki Reservoir (Cracow, Poland) in summer and winter seasons. In total, 94 strains, identified as E. coli, were isolated from five sampling sites in the area of the reservoir. Based on the disk-diffusion tests, it was found that the tested isolates were predominantly resistant to ticarcillin and ampicillin. Numerous multi-drug resistant strains were detected, which however did not exhibit the ESBL phenotype. However, PCR approach allowed to detect ESBL-mechanism genes (CTX-M3, OXA, SHV, and TEM) in as much as 38 % of E. coli isolates. These results were coupled with significant molecular diversity of the E. coli strains revealed in BOXA1R-based rep-PCR technique. |
format | Online Article Text |
id | pubmed-4835524 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-48355242016-05-04 Phenotypic and Molecular Assessment of Drug Resistance Profile and Genetic Diversity of Waterborne Escherichia coli Wolny-Koładka, Katarzyna Lenart-Boroń, Anna Water Air Soil Pollut Article Bacterial Escherichia coli isolates were derived from waters of the Nowohucki Reservoir (Cracow, Poland) in summer and winter seasons. In total, 94 strains, identified as E. coli, were isolated from five sampling sites in the area of the reservoir. Based on the disk-diffusion tests, it was found that the tested isolates were predominantly resistant to ticarcillin and ampicillin. Numerous multi-drug resistant strains were detected, which however did not exhibit the ESBL phenotype. However, PCR approach allowed to detect ESBL-mechanism genes (CTX-M3, OXA, SHV, and TEM) in as much as 38 % of E. coli isolates. These results were coupled with significant molecular diversity of the E. coli strains revealed in BOXA1R-based rep-PCR technique. Springer International Publishing 2016-04-19 2016 /pmc/articles/PMC4835524/ /pubmed/27158170 http://dx.doi.org/10.1007/s11270-016-2833-z Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Article Wolny-Koładka, Katarzyna Lenart-Boroń, Anna Phenotypic and Molecular Assessment of Drug Resistance Profile and Genetic Diversity of Waterborne Escherichia coli |
title | Phenotypic and Molecular Assessment of Drug Resistance Profile and Genetic Diversity of Waterborne Escherichia coli |
title_full | Phenotypic and Molecular Assessment of Drug Resistance Profile and Genetic Diversity of Waterborne Escherichia coli |
title_fullStr | Phenotypic and Molecular Assessment of Drug Resistance Profile and Genetic Diversity of Waterborne Escherichia coli |
title_full_unstemmed | Phenotypic and Molecular Assessment of Drug Resistance Profile and Genetic Diversity of Waterborne Escherichia coli |
title_short | Phenotypic and Molecular Assessment of Drug Resistance Profile and Genetic Diversity of Waterborne Escherichia coli |
title_sort | phenotypic and molecular assessment of drug resistance profile and genetic diversity of waterborne escherichia coli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835524/ https://www.ncbi.nlm.nih.gov/pubmed/27158170 http://dx.doi.org/10.1007/s11270-016-2833-z |
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