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Somatic embryogenesis and enhanced shoot organogenesis in Metabriggsia ovalifolia W. T. Wang

An efficient protocol providing a dual regeneration pathway via direct shoot organogenesis and somatic embryogenesis for an endangered species, Metabriggsia ovalifolia W. T. Wang, was established from leaf explants. When applied at 2.5 μM, the cytokinins 6-benzyladenine (BA) or thidiazuron (TDZ) and...

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Autores principales: Ouyang, Yao, Chen, Yulu, Lü, Jinfeng, Teixeira da Silva, Jaime A., Zhang, Xinhua, Ma, Guohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835782/
https://www.ncbi.nlm.nih.gov/pubmed/27090564
http://dx.doi.org/10.1038/srep24662
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author Ouyang, Yao
Chen, Yulu
Lü, Jinfeng
Teixeira da Silva, Jaime A.
Zhang, Xinhua
Ma, Guohua
author_facet Ouyang, Yao
Chen, Yulu
Lü, Jinfeng
Teixeira da Silva, Jaime A.
Zhang, Xinhua
Ma, Guohua
author_sort Ouyang, Yao
collection PubMed
description An efficient protocol providing a dual regeneration pathway via direct shoot organogenesis and somatic embryogenesis for an endangered species, Metabriggsia ovalifolia W. T. Wang, was established from leaf explants. When applied at 2.5 μM, the cytokinins 6-benzyladenine (BA) or thidiazuron (TDZ) and the auxins indole-3-butyric acid (IBA), α-naphthaleneacetic acid (NAA) and indole-3-acetic acid (IAA) could induce shoots when on basal Murashige and Skoog (MS) medium. BA and TDZ could induce more adventitious shoots (19.1 and 31.2/explant, respectively) than NAA (4.6/explant), IBA (5.7/explant) or IAA (6.4/explant). BA and TDZ at 5–10 μM could induce both shoots and somatic embryos. A higher concentration of TDZ (25 μM) induced only somatic embryos (39.8/explant). The same concentration of BA induced both adventitious shoots (23.6/explant) and somatic embryos (9.7/explant). Thus, somatic embryogenesis in this plant needs a high cytokinin concentration (BA; TDZ), as evidenced by histology. Somatic embryos germinated easily when left on the same media, but formed adventitious roots in two weeks on MS supplemented with 0.5 μM NAA, 0.5 μM IBA and 0.1% activated charcoal. Over 93% of plantlets survived following acclimatization and transfer to a mixture of sand and vermiculite (1:1, v/v) in trays.
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spelling pubmed-48357822016-04-27 Somatic embryogenesis and enhanced shoot organogenesis in Metabriggsia ovalifolia W. T. Wang Ouyang, Yao Chen, Yulu Lü, Jinfeng Teixeira da Silva, Jaime A. Zhang, Xinhua Ma, Guohua Sci Rep Article An efficient protocol providing a dual regeneration pathway via direct shoot organogenesis and somatic embryogenesis for an endangered species, Metabriggsia ovalifolia W. T. Wang, was established from leaf explants. When applied at 2.5 μM, the cytokinins 6-benzyladenine (BA) or thidiazuron (TDZ) and the auxins indole-3-butyric acid (IBA), α-naphthaleneacetic acid (NAA) and indole-3-acetic acid (IAA) could induce shoots when on basal Murashige and Skoog (MS) medium. BA and TDZ could induce more adventitious shoots (19.1 and 31.2/explant, respectively) than NAA (4.6/explant), IBA (5.7/explant) or IAA (6.4/explant). BA and TDZ at 5–10 μM could induce both shoots and somatic embryos. A higher concentration of TDZ (25 μM) induced only somatic embryos (39.8/explant). The same concentration of BA induced both adventitious shoots (23.6/explant) and somatic embryos (9.7/explant). Thus, somatic embryogenesis in this plant needs a high cytokinin concentration (BA; TDZ), as evidenced by histology. Somatic embryos germinated easily when left on the same media, but formed adventitious roots in two weeks on MS supplemented with 0.5 μM NAA, 0.5 μM IBA and 0.1% activated charcoal. Over 93% of plantlets survived following acclimatization and transfer to a mixture of sand and vermiculite (1:1, v/v) in trays. Nature Publishing Group 2016-04-19 /pmc/articles/PMC4835782/ /pubmed/27090564 http://dx.doi.org/10.1038/srep24662 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ouyang, Yao
Chen, Yulu
Lü, Jinfeng
Teixeira da Silva, Jaime A.
Zhang, Xinhua
Ma, Guohua
Somatic embryogenesis and enhanced shoot organogenesis in Metabriggsia ovalifolia W. T. Wang
title Somatic embryogenesis and enhanced shoot organogenesis in Metabriggsia ovalifolia W. T. Wang
title_full Somatic embryogenesis and enhanced shoot organogenesis in Metabriggsia ovalifolia W. T. Wang
title_fullStr Somatic embryogenesis and enhanced shoot organogenesis in Metabriggsia ovalifolia W. T. Wang
title_full_unstemmed Somatic embryogenesis and enhanced shoot organogenesis in Metabriggsia ovalifolia W. T. Wang
title_short Somatic embryogenesis and enhanced shoot organogenesis in Metabriggsia ovalifolia W. T. Wang
title_sort somatic embryogenesis and enhanced shoot organogenesis in metabriggsia ovalifolia w. t. wang
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835782/
https://www.ncbi.nlm.nih.gov/pubmed/27090564
http://dx.doi.org/10.1038/srep24662
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