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The effects of Bt Cry1Ie toxin on bacterial diversity in the midgut of Apis mellifera ligustica (Hymenoptera: Apidae)

The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops. Here, the potential adverse effects of Cry1Ie toxin on the midgut bacteria of the worker bees (Apis mellifera ligustica) were investigated under laboratory conditions. Newly emerged bees were fed...

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Autores principales: Jia, Hui-Ru, Geng, Li-Li, Li, Yun-He, Wang, Qiang, Diao, Qing-Yun, Zhou, Ting, Dai, Ping-Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835784/
https://www.ncbi.nlm.nih.gov/pubmed/27090812
http://dx.doi.org/10.1038/srep24664
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author Jia, Hui-Ru
Geng, Li-Li
Li, Yun-He
Wang, Qiang
Diao, Qing-Yun
Zhou, Ting
Dai, Ping-Li
author_facet Jia, Hui-Ru
Geng, Li-Li
Li, Yun-He
Wang, Qiang
Diao, Qing-Yun
Zhou, Ting
Dai, Ping-Li
author_sort Jia, Hui-Ru
collection PubMed
description The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops. Here, the potential adverse effects of Cry1Ie toxin on the midgut bacteria of the worker bees (Apis mellifera ligustica) were investigated under laboratory conditions. Newly emerged bees were fed with different concentrations of Cry1Ie toxin syrups (20 ng/mL, 200 ng/mL, and 20 μg/mL), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d. We characterized the dominant midgut bacteria and compared the composition and structure of the midgut bacterial community in all samples using the Illumina MiSeq platform targeting the V3–V4 regions of 16S rDNA. No significant differences in the diversity of the midgut bacteria were observed between the five treatments. This work was the first to show the effects of Cry1Ie toxin on honey bees, and our study provided a theoretical basis for the biosafety assessment of transgenic Cry1Ie maize.
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spelling pubmed-48357842016-04-27 The effects of Bt Cry1Ie toxin on bacterial diversity in the midgut of Apis mellifera ligustica (Hymenoptera: Apidae) Jia, Hui-Ru Geng, Li-Li Li, Yun-He Wang, Qiang Diao, Qing-Yun Zhou, Ting Dai, Ping-Li Sci Rep Article The honey bee has been regarded as a key species in the environmental risk assessment of biotech crops. Here, the potential adverse effects of Cry1Ie toxin on the midgut bacteria of the worker bees (Apis mellifera ligustica) were investigated under laboratory conditions. Newly emerged bees were fed with different concentrations of Cry1Ie toxin syrups (20 ng/mL, 200 ng/mL, and 20 μg/mL), pure sugar syrup, and 48 ppb of imidacloprid syrups, then sampled after 15 and 30 d. We characterized the dominant midgut bacteria and compared the composition and structure of the midgut bacterial community in all samples using the Illumina MiSeq platform targeting the V3–V4 regions of 16S rDNA. No significant differences in the diversity of the midgut bacteria were observed between the five treatments. This work was the first to show the effects of Cry1Ie toxin on honey bees, and our study provided a theoretical basis for the biosafety assessment of transgenic Cry1Ie maize. Nature Publishing Group 2016-04-19 /pmc/articles/PMC4835784/ /pubmed/27090812 http://dx.doi.org/10.1038/srep24664 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Jia, Hui-Ru
Geng, Li-Li
Li, Yun-He
Wang, Qiang
Diao, Qing-Yun
Zhou, Ting
Dai, Ping-Li
The effects of Bt Cry1Ie toxin on bacterial diversity in the midgut of Apis mellifera ligustica (Hymenoptera: Apidae)
title The effects of Bt Cry1Ie toxin on bacterial diversity in the midgut of Apis mellifera ligustica (Hymenoptera: Apidae)
title_full The effects of Bt Cry1Ie toxin on bacterial diversity in the midgut of Apis mellifera ligustica (Hymenoptera: Apidae)
title_fullStr The effects of Bt Cry1Ie toxin on bacterial diversity in the midgut of Apis mellifera ligustica (Hymenoptera: Apidae)
title_full_unstemmed The effects of Bt Cry1Ie toxin on bacterial diversity in the midgut of Apis mellifera ligustica (Hymenoptera: Apidae)
title_short The effects of Bt Cry1Ie toxin on bacterial diversity in the midgut of Apis mellifera ligustica (Hymenoptera: Apidae)
title_sort effects of bt cry1ie toxin on bacterial diversity in the midgut of apis mellifera ligustica (hymenoptera: apidae)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835784/
https://www.ncbi.nlm.nih.gov/pubmed/27090812
http://dx.doi.org/10.1038/srep24664
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