Cargando…

Renal effects of angiotensin II in the newborn period: role of type 1 and type 2 receptors

BACKGROUND: Evidence suggests a critical role for the renin-angiotensin system in regulating renal function during postnatal development. However, the physiological relevance of a highly elevated renin-angiotensin system early in life is not well understood, nor which angiotensin receptors might be...

Descripción completa

Detalles Bibliográficos
Autores principales: Vinturache, Angela E., Smith, Francine G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835895/
https://www.ncbi.nlm.nih.gov/pubmed/27090941
http://dx.doi.org/10.1186/s12899-016-0022-3
_version_ 1782427692774195200
author Vinturache, Angela E.
Smith, Francine G.
author_facet Vinturache, Angela E.
Smith, Francine G.
author_sort Vinturache, Angela E.
collection PubMed
description BACKGROUND: Evidence suggests a critical role for the renin-angiotensin system in regulating renal function during postnatal development. However, the physiological relevance of a highly elevated renin-angiotensin system early in life is not well understood, nor which angiotensin receptors might be involved. This study was designed to investigate the roles of angiotensin receptors type 1 (AT1R) and type 2 (AT2R) in regulating glomerular and tubular function during postnatal development. METHODS: The renal effects of the selective antagonist to AT1R, ZD 7155 and to AT2R, PD 1233319 were evaluated in two groups of conscious chronically instrumented lambs aged ~ one week (N = 8) and ~ six weeks (N = 10). Two experiments were carried out in each animal and consisted of the assessment of renal variables including glomerular and tubular function, for 30 min before (Control) and 60 min after infusion of ZD 7155 and PD 123319, respectively. Statistical significance was determined using parametric testing (Student t-test, analysis of variance ANOVA) as appropriate. RESULTS: ZD 7155 infusion was associated with a significant decrease in glomerular filtration rate and filtration fraction at one but not six weeks; urinary flow rate decreased significantly in older animals, whereas sodium excretion and free water clearance were not altered. There was an age-dependent effect on potassium handling along the nephron, potassium excretion decreasing after ZD 7155 infusion in younger but not in older lambs. PD 123319 had no significant effects on glomerular filtration rate and tubular function in either age group. CONCLUSIONS: These results provide evidence to support an important role for AT1Rs in mediating the renal effects of angiotensin II during postnatal maturation in conscious developing animals. In contrast to a role for AT2Rs later in life, there appears to be no role for AT2Rs in influencing the renal effects of Angiotensin II in the postnatal period. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12899-016-0022-3) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4835895
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-48358952016-04-20 Renal effects of angiotensin II in the newborn period: role of type 1 and type 2 receptors Vinturache, Angela E. Smith, Francine G. BMC Physiol Research Article BACKGROUND: Evidence suggests a critical role for the renin-angiotensin system in regulating renal function during postnatal development. However, the physiological relevance of a highly elevated renin-angiotensin system early in life is not well understood, nor which angiotensin receptors might be involved. This study was designed to investigate the roles of angiotensin receptors type 1 (AT1R) and type 2 (AT2R) in regulating glomerular and tubular function during postnatal development. METHODS: The renal effects of the selective antagonist to AT1R, ZD 7155 and to AT2R, PD 1233319 were evaluated in two groups of conscious chronically instrumented lambs aged ~ one week (N = 8) and ~ six weeks (N = 10). Two experiments were carried out in each animal and consisted of the assessment of renal variables including glomerular and tubular function, for 30 min before (Control) and 60 min after infusion of ZD 7155 and PD 123319, respectively. Statistical significance was determined using parametric testing (Student t-test, analysis of variance ANOVA) as appropriate. RESULTS: ZD 7155 infusion was associated with a significant decrease in glomerular filtration rate and filtration fraction at one but not six weeks; urinary flow rate decreased significantly in older animals, whereas sodium excretion and free water clearance were not altered. There was an age-dependent effect on potassium handling along the nephron, potassium excretion decreasing after ZD 7155 infusion in younger but not in older lambs. PD 123319 had no significant effects on glomerular filtration rate and tubular function in either age group. CONCLUSIONS: These results provide evidence to support an important role for AT1Rs in mediating the renal effects of angiotensin II during postnatal maturation in conscious developing animals. In contrast to a role for AT2Rs later in life, there appears to be no role for AT2Rs in influencing the renal effects of Angiotensin II in the postnatal period. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12899-016-0022-3) contains supplementary material, which is available to authorized users. BioMed Central 2016-04-18 /pmc/articles/PMC4835895/ /pubmed/27090941 http://dx.doi.org/10.1186/s12899-016-0022-3 Text en © Vinturache and Smith. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Vinturache, Angela E.
Smith, Francine G.
Renal effects of angiotensin II in the newborn period: role of type 1 and type 2 receptors
title Renal effects of angiotensin II in the newborn period: role of type 1 and type 2 receptors
title_full Renal effects of angiotensin II in the newborn period: role of type 1 and type 2 receptors
title_fullStr Renal effects of angiotensin II in the newborn period: role of type 1 and type 2 receptors
title_full_unstemmed Renal effects of angiotensin II in the newborn period: role of type 1 and type 2 receptors
title_short Renal effects of angiotensin II in the newborn period: role of type 1 and type 2 receptors
title_sort renal effects of angiotensin ii in the newborn period: role of type 1 and type 2 receptors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4835895/
https://www.ncbi.nlm.nih.gov/pubmed/27090941
http://dx.doi.org/10.1186/s12899-016-0022-3
work_keys_str_mv AT vinturacheangelae renaleffectsofangiotensiniiinthenewbornperiodroleoftype1andtype2receptors
AT smithfrancineg renaleffectsofangiotensiniiinthenewbornperiodroleoftype1andtype2receptors