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Genome-Wide Identification and Function Analyses of Heat Shock Transcription Factors in Potato

Heat shock transcription factors (Hsfs) play vital roles in the regulation of tolerance to various stresses in living organisms. To dissect the mechanisms of the Hsfs in potato adaptation to abiotic stresses, genome and transcriptome analyses of Hsf gene family were investigated in Solanum tuberosum...

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Autores principales: Tang, Ruimin, Zhu, Wenjiao, Song, Xiaoyan, Lin, Xingzhong, Cai, Jinghui, Wang, Man, Yang, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836240/
https://www.ncbi.nlm.nih.gov/pubmed/27148315
http://dx.doi.org/10.3389/fpls.2016.00490
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author Tang, Ruimin
Zhu, Wenjiao
Song, Xiaoyan
Lin, Xingzhong
Cai, Jinghui
Wang, Man
Yang, Qing
author_facet Tang, Ruimin
Zhu, Wenjiao
Song, Xiaoyan
Lin, Xingzhong
Cai, Jinghui
Wang, Man
Yang, Qing
author_sort Tang, Ruimin
collection PubMed
description Heat shock transcription factors (Hsfs) play vital roles in the regulation of tolerance to various stresses in living organisms. To dissect the mechanisms of the Hsfs in potato adaptation to abiotic stresses, genome and transcriptome analyses of Hsf gene family were investigated in Solanum tuberosum L. Twenty-seven StHsf members were identified by bioinformatics and phylogenetic analyses and were classified into A, B, and C groups according to their structural and phylogenetic features. StHsfs in the same class shared similar gene structures and conserved motifs. The chromosomal location analysis showed that 27 Hsfs were located in 10 of 12 chromosomes (except chromosome 1 and chromosome 5) and that 18 of these genes formed 9 paralogous pairs. Expression profiles of StHsfs in 12 different organs and tissues uncovered distinct spatial expression patterns of these genes and their potential roles in the process of growth and development. Promoter and quantitative real-time polymerase chain reaction (qRT-PCR) detections of StHsfs were conducted and demonstrated that these genes were all responsive to various stresses. StHsf004, StHsf007, StHsf009, StHsf014, and StHsf019 were constitutively expressed under non-stress conditions, and some specific Hsfs became the predominant Hsfs in response to different abiotic stresses, indicating their important and diverse regulatory roles in adverse conditions. A co-expression network between StHsfs and StHsf -co-expressed genes was generated based on the publicly-available potato transcriptomic databases and identified key candidate StHsfs for further functional studies.
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spelling pubmed-48362402016-05-04 Genome-Wide Identification and Function Analyses of Heat Shock Transcription Factors in Potato Tang, Ruimin Zhu, Wenjiao Song, Xiaoyan Lin, Xingzhong Cai, Jinghui Wang, Man Yang, Qing Front Plant Sci Plant Science Heat shock transcription factors (Hsfs) play vital roles in the regulation of tolerance to various stresses in living organisms. To dissect the mechanisms of the Hsfs in potato adaptation to abiotic stresses, genome and transcriptome analyses of Hsf gene family were investigated in Solanum tuberosum L. Twenty-seven StHsf members were identified by bioinformatics and phylogenetic analyses and were classified into A, B, and C groups according to their structural and phylogenetic features. StHsfs in the same class shared similar gene structures and conserved motifs. The chromosomal location analysis showed that 27 Hsfs were located in 10 of 12 chromosomes (except chromosome 1 and chromosome 5) and that 18 of these genes formed 9 paralogous pairs. Expression profiles of StHsfs in 12 different organs and tissues uncovered distinct spatial expression patterns of these genes and their potential roles in the process of growth and development. Promoter and quantitative real-time polymerase chain reaction (qRT-PCR) detections of StHsfs were conducted and demonstrated that these genes were all responsive to various stresses. StHsf004, StHsf007, StHsf009, StHsf014, and StHsf019 were constitutively expressed under non-stress conditions, and some specific Hsfs became the predominant Hsfs in response to different abiotic stresses, indicating their important and diverse regulatory roles in adverse conditions. A co-expression network between StHsfs and StHsf -co-expressed genes was generated based on the publicly-available potato transcriptomic databases and identified key candidate StHsfs for further functional studies. Frontiers Media S.A. 2016-04-19 /pmc/articles/PMC4836240/ /pubmed/27148315 http://dx.doi.org/10.3389/fpls.2016.00490 Text en Copyright © 2016 Tang, Zhu, Song, Lin, Cai, Wang and Yang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Tang, Ruimin
Zhu, Wenjiao
Song, Xiaoyan
Lin, Xingzhong
Cai, Jinghui
Wang, Man
Yang, Qing
Genome-Wide Identification and Function Analyses of Heat Shock Transcription Factors in Potato
title Genome-Wide Identification and Function Analyses of Heat Shock Transcription Factors in Potato
title_full Genome-Wide Identification and Function Analyses of Heat Shock Transcription Factors in Potato
title_fullStr Genome-Wide Identification and Function Analyses of Heat Shock Transcription Factors in Potato
title_full_unstemmed Genome-Wide Identification and Function Analyses of Heat Shock Transcription Factors in Potato
title_short Genome-Wide Identification and Function Analyses of Heat Shock Transcription Factors in Potato
title_sort genome-wide identification and function analyses of heat shock transcription factors in potato
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836240/
https://www.ncbi.nlm.nih.gov/pubmed/27148315
http://dx.doi.org/10.3389/fpls.2016.00490
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