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A MALDI-MS-based quantitative analytical method for endogenous estrone in human breast cancer cells
The level of endogenous estrone, one of the three major naturally occurring estrogens, has a significant correlation with the incidence of post-menopausal breast cancer. However, it is challenging to quantitatively monitor it owing to its low abundance. Here, we develop a robust and highly sensitive...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836303/ https://www.ncbi.nlm.nih.gov/pubmed/27091422 http://dx.doi.org/10.1038/srep24489 |
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author | Kim, Kyoung-Jin Kim, Hee-Jin Park, Han-Gyu Hwang, Cheol-Hwan Sung, Changmin Jang, Kyoung-Soon Park, Sung-Hee Kim, Byung-Gee Lee, Yoo-Kyung Yang, Yung-Hun Jeong, Jae Hyun Kim, Yun-Gon |
author_facet | Kim, Kyoung-Jin Kim, Hee-Jin Park, Han-Gyu Hwang, Cheol-Hwan Sung, Changmin Jang, Kyoung-Soon Park, Sung-Hee Kim, Byung-Gee Lee, Yoo-Kyung Yang, Yung-Hun Jeong, Jae Hyun Kim, Yun-Gon |
author_sort | Kim, Kyoung-Jin |
collection | PubMed |
description | The level of endogenous estrone, one of the three major naturally occurring estrogens, has a significant correlation with the incidence of post-menopausal breast cancer. However, it is challenging to quantitatively monitor it owing to its low abundance. Here, we develop a robust and highly sensitive mass-assisted laser desorption/ionization mass spectrometry (MALDI-MS)-based quantitative platform to identify the absolute quantities of endogenous estrones in a variety of clinical specimens. The one-step modification of endogenous estrone provided good linearity (R(2) > 0.99) and significantly increased the sensitivity of the platform (limit of quantitation: 11 fmol). In addition, we could identify the absolute amount of endogenous estrones in cells of the breast cancer cell line MCF-7 (34 fmol/10(6) cells) by using a deuterated estrone as an internal standard. Finally, by applying the MALDI-MS-based quantitative method to endogenous estrones, we successfully monitored changes in the metabolic expression level of estrones (17.7 fmol/10(6) letrozole-treated cells) in MCF-7 cells resulting from treatment with an aromatase inhibitor. Taken together, these results suggest that this MALDI-MS-based quantitative approach may be a general method for the targeted metabolomics of ketone-containing metabolites, which can reflect clinical conditions and pathogenic mechanisms. |
format | Online Article Text |
id | pubmed-4836303 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48363032016-04-27 A MALDI-MS-based quantitative analytical method for endogenous estrone in human breast cancer cells Kim, Kyoung-Jin Kim, Hee-Jin Park, Han-Gyu Hwang, Cheol-Hwan Sung, Changmin Jang, Kyoung-Soon Park, Sung-Hee Kim, Byung-Gee Lee, Yoo-Kyung Yang, Yung-Hun Jeong, Jae Hyun Kim, Yun-Gon Sci Rep Article The level of endogenous estrone, one of the three major naturally occurring estrogens, has a significant correlation with the incidence of post-menopausal breast cancer. However, it is challenging to quantitatively monitor it owing to its low abundance. Here, we develop a robust and highly sensitive mass-assisted laser desorption/ionization mass spectrometry (MALDI-MS)-based quantitative platform to identify the absolute quantities of endogenous estrones in a variety of clinical specimens. The one-step modification of endogenous estrone provided good linearity (R(2) > 0.99) and significantly increased the sensitivity of the platform (limit of quantitation: 11 fmol). In addition, we could identify the absolute amount of endogenous estrones in cells of the breast cancer cell line MCF-7 (34 fmol/10(6) cells) by using a deuterated estrone as an internal standard. Finally, by applying the MALDI-MS-based quantitative method to endogenous estrones, we successfully monitored changes in the metabolic expression level of estrones (17.7 fmol/10(6) letrozole-treated cells) in MCF-7 cells resulting from treatment with an aromatase inhibitor. Taken together, these results suggest that this MALDI-MS-based quantitative approach may be a general method for the targeted metabolomics of ketone-containing metabolites, which can reflect clinical conditions and pathogenic mechanisms. Nature Publishing Group 2016-04-19 /pmc/articles/PMC4836303/ /pubmed/27091422 http://dx.doi.org/10.1038/srep24489 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Kim, Kyoung-Jin Kim, Hee-Jin Park, Han-Gyu Hwang, Cheol-Hwan Sung, Changmin Jang, Kyoung-Soon Park, Sung-Hee Kim, Byung-Gee Lee, Yoo-Kyung Yang, Yung-Hun Jeong, Jae Hyun Kim, Yun-Gon A MALDI-MS-based quantitative analytical method for endogenous estrone in human breast cancer cells |
title | A MALDI-MS-based quantitative analytical method for endogenous estrone in human breast cancer cells |
title_full | A MALDI-MS-based quantitative analytical method for endogenous estrone in human breast cancer cells |
title_fullStr | A MALDI-MS-based quantitative analytical method for endogenous estrone in human breast cancer cells |
title_full_unstemmed | A MALDI-MS-based quantitative analytical method for endogenous estrone in human breast cancer cells |
title_short | A MALDI-MS-based quantitative analytical method for endogenous estrone in human breast cancer cells |
title_sort | maldi-ms-based quantitative analytical method for endogenous estrone in human breast cancer cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836303/ https://www.ncbi.nlm.nih.gov/pubmed/27091422 http://dx.doi.org/10.1038/srep24489 |
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