Ubiquitous accumulation of 3′ mRNA decay fragments in Saccharomyces cerevisiae mRNAs with chromosomally integrated MS2 arrays

The binding of MS2-GFP protein to arrays of MS2 sites in yeast mRNAs has been used extensively to visualize mRNA localization. We previously reported that arrays of MS2 sites bound by MS2 protein could inhibit Xrn1p and lead to the accumulation of 3′ mRNA decay fragments. We suggest that these decay...

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Detalles Bibliográficos
Autores principales: Garcia, Jennifer F., Parker, Roy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2016
Materias:
Commentary
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836640/
https://www.ncbi.nlm.nih.gov/pubmed/27090788
http://dx.doi.org/10.1261/rna.056325.116
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author Garcia, Jennifer F.
Parker, Roy
author_facet Garcia, Jennifer F.
Parker, Roy
author_sort Garcia, Jennifer F.
collection PubMed
description The binding of MS2-GFP protein to arrays of MS2 sites in yeast mRNAs has been used extensively to visualize mRNA localization. We previously reported that arrays of MS2 sites bound by MS2 protein could inhibit Xrn1p and lead to the accumulation of 3′ mRNA decay fragments. We suggest that these decay fragments have the potential to complicate mRNA localization studies, as stated in an earlier study.
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spelling pubmed-48366402017-05-01 Ubiquitous accumulation of 3′ mRNA decay fragments in Saccharomyces cerevisiae mRNAs with chromosomally integrated MS2 arrays Garcia, Jennifer F. Parker, Roy RNA Commentary The binding of MS2-GFP protein to arrays of MS2 sites in yeast mRNAs has been used extensively to visualize mRNA localization. We previously reported that arrays of MS2 sites bound by MS2 protein could inhibit Xrn1p and lead to the accumulation of 3′ mRNA decay fragments. We suggest that these decay fragments have the potential to complicate mRNA localization studies, as stated in an earlier study. Cold Spring Harbor Laboratory Press 2016-05 /pmc/articles/PMC4836640/ /pubmed/27090788 http://dx.doi.org/10.1261/rna.056325.116 Text en © 2016 Garcia and Parker; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Commentary
Garcia, Jennifer F.
Parker, Roy
Ubiquitous accumulation of 3′ mRNA decay fragments in Saccharomyces cerevisiae mRNAs with chromosomally integrated MS2 arrays
title Ubiquitous accumulation of 3′ mRNA decay fragments in Saccharomyces cerevisiae mRNAs with chromosomally integrated MS2 arrays
title_full Ubiquitous accumulation of 3′ mRNA decay fragments in Saccharomyces cerevisiae mRNAs with chromosomally integrated MS2 arrays
title_fullStr Ubiquitous accumulation of 3′ mRNA decay fragments in Saccharomyces cerevisiae mRNAs with chromosomally integrated MS2 arrays
title_full_unstemmed Ubiquitous accumulation of 3′ mRNA decay fragments in Saccharomyces cerevisiae mRNAs with chromosomally integrated MS2 arrays
title_short Ubiquitous accumulation of 3′ mRNA decay fragments in Saccharomyces cerevisiae mRNAs with chromosomally integrated MS2 arrays
title_sort ubiquitous accumulation of 3′ mrna decay fragments in saccharomyces cerevisiae mrnas with chromosomally integrated ms2 arrays
topic Commentary
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836640/
https://www.ncbi.nlm.nih.gov/pubmed/27090788
http://dx.doi.org/10.1261/rna.056325.116
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AT parkerroy ubiquitousaccumulationof3mrnadecayfragmentsinsaccharomycescerevisiaemrnaswithchromosomallyintegratedms2arrays

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