Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to “MS2 coat proteins bound to yeast mRNAs block 5′ to 3′ degradation and trap mRNA decay products: implications for the localization of mRNAs by MS2-MCP system”

The MS2 system has been extensively used to visualize single mRNA molecules in live cells and follow their localization and behavior. In their Letter to the Editor recently published, Garcia and Parker suggest that use of the MS2 system may yield erroneous mRNA localization results due to the accumu...

Descripción completa

Detalles Bibliográficos
Autores principales: Haimovich, Gal, Zabezhinsky, Dmitry, Haas, Brian, Slobodin, Boris, Purushothaman, Pravinkumar, Fan, Lin, Levin, Joshua Z., Nusbaum, Chad, Gerst, Jeffrey E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2016
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836641/
https://www.ncbi.nlm.nih.gov/pubmed/26968626
http://dx.doi.org/10.1261/rna.055095.115
_version_ 1782427755626889216
author Haimovich, Gal
Zabezhinsky, Dmitry
Haas, Brian
Slobodin, Boris
Purushothaman, Pravinkumar
Fan, Lin
Levin, Joshua Z.
Nusbaum, Chad
Gerst, Jeffrey E.
author_facet Haimovich, Gal
Zabezhinsky, Dmitry
Haas, Brian
Slobodin, Boris
Purushothaman, Pravinkumar
Fan, Lin
Levin, Joshua Z.
Nusbaum, Chad
Gerst, Jeffrey E.
author_sort Haimovich, Gal
collection PubMed
description The MS2 system has been extensively used to visualize single mRNA molecules in live cells and follow their localization and behavior. In their Letter to the Editor recently published, Garcia and Parker suggest that use of the MS2 system may yield erroneous mRNA localization results due to the accumulation of 3′ decay products. Here we cite published works and provide new data which demonstrate that this is not a phenomenon general to endogenously expressed MS2-tagged transcripts, and that some of the results obtained in their study could have arisen from artifacts of gene expression.
format Online
Article
Text
id pubmed-4836641
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Cold Spring Harbor Laboratory Press
record_format MEDLINE/PubMed
spelling pubmed-48366412017-05-01 Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to “MS2 coat proteins bound to yeast mRNAs block 5′ to 3′ degradation and trap mRNA decay products: implications for the localization of mRNAs by MS2-MCP system” Haimovich, Gal Zabezhinsky, Dmitry Haas, Brian Slobodin, Boris Purushothaman, Pravinkumar Fan, Lin Levin, Joshua Z. Nusbaum, Chad Gerst, Jeffrey E. RNA Letter to the Editor The MS2 system has been extensively used to visualize single mRNA molecules in live cells and follow their localization and behavior. In their Letter to the Editor recently published, Garcia and Parker suggest that use of the MS2 system may yield erroneous mRNA localization results due to the accumulation of 3′ decay products. Here we cite published works and provide new data which demonstrate that this is not a phenomenon general to endogenously expressed MS2-tagged transcripts, and that some of the results obtained in their study could have arisen from artifacts of gene expression. Cold Spring Harbor Laboratory Press 2016-05 /pmc/articles/PMC4836641/ /pubmed/26968626 http://dx.doi.org/10.1261/rna.055095.115 Text en © 2016 Haimovich et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Letter to the Editor
Haimovich, Gal
Zabezhinsky, Dmitry
Haas, Brian
Slobodin, Boris
Purushothaman, Pravinkumar
Fan, Lin
Levin, Joshua Z.
Nusbaum, Chad
Gerst, Jeffrey E.
Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to “MS2 coat proteins bound to yeast mRNAs block 5′ to 3′ degradation and trap mRNA decay products: implications for the localization of mRNAs by MS2-MCP system”
title Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to “MS2 coat proteins bound to yeast mRNAs block 5′ to 3′ degradation and trap mRNA decay products: implications for the localization of mRNAs by MS2-MCP system”
title_full Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to “MS2 coat proteins bound to yeast mRNAs block 5′ to 3′ degradation and trap mRNA decay products: implications for the localization of mRNAs by MS2-MCP system”
title_fullStr Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to “MS2 coat proteins bound to yeast mRNAs block 5′ to 3′ degradation and trap mRNA decay products: implications for the localization of mRNAs by MS2-MCP system”
title_full_unstemmed Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to “MS2 coat proteins bound to yeast mRNAs block 5′ to 3′ degradation and trap mRNA decay products: implications for the localization of mRNAs by MS2-MCP system”
title_short Use of the MS2 aptamer and coat protein for RNA localization in yeast: A response to “MS2 coat proteins bound to yeast mRNAs block 5′ to 3′ degradation and trap mRNA decay products: implications for the localization of mRNAs by MS2-MCP system”
title_sort use of the ms2 aptamer and coat protein for rna localization in yeast: a response to “ms2 coat proteins bound to yeast mrnas block 5′ to 3′ degradation and trap mrna decay products: implications for the localization of mrnas by ms2-mcp system”
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836641/
https://www.ncbi.nlm.nih.gov/pubmed/26968626
http://dx.doi.org/10.1261/rna.055095.115
work_keys_str_mv AT haimovichgal useofthems2aptamerandcoatproteinforrnalocalizationinyeastaresponsetoms2coatproteinsboundtoyeastmrnasblock5to3degradationandtrapmrnadecayproductsimplicationsforthelocalizationofmrnasbyms2mcpsystem
AT zabezhinskydmitry useofthems2aptamerandcoatproteinforrnalocalizationinyeastaresponsetoms2coatproteinsboundtoyeastmrnasblock5to3degradationandtrapmrnadecayproductsimplicationsforthelocalizationofmrnasbyms2mcpsystem
AT haasbrian useofthems2aptamerandcoatproteinforrnalocalizationinyeastaresponsetoms2coatproteinsboundtoyeastmrnasblock5to3degradationandtrapmrnadecayproductsimplicationsforthelocalizationofmrnasbyms2mcpsystem
AT slobodinboris useofthems2aptamerandcoatproteinforrnalocalizationinyeastaresponsetoms2coatproteinsboundtoyeastmrnasblock5to3degradationandtrapmrnadecayproductsimplicationsforthelocalizationofmrnasbyms2mcpsystem
AT purushothamanpravinkumar useofthems2aptamerandcoatproteinforrnalocalizationinyeastaresponsetoms2coatproteinsboundtoyeastmrnasblock5to3degradationandtrapmrnadecayproductsimplicationsforthelocalizationofmrnasbyms2mcpsystem
AT fanlin useofthems2aptamerandcoatproteinforrnalocalizationinyeastaresponsetoms2coatproteinsboundtoyeastmrnasblock5to3degradationandtrapmrnadecayproductsimplicationsforthelocalizationofmrnasbyms2mcpsystem
AT levinjoshuaz useofthems2aptamerandcoatproteinforrnalocalizationinyeastaresponsetoms2coatproteinsboundtoyeastmrnasblock5to3degradationandtrapmrnadecayproductsimplicationsforthelocalizationofmrnasbyms2mcpsystem
AT nusbaumchad useofthems2aptamerandcoatproteinforrnalocalizationinyeastaresponsetoms2coatproteinsboundtoyeastmrnasblock5to3degradationandtrapmrnadecayproductsimplicationsforthelocalizationofmrnasbyms2mcpsystem
AT gerstjeffreye useofthems2aptamerandcoatproteinforrnalocalizationinyeastaresponsetoms2coatproteinsboundtoyeastmrnasblock5to3degradationandtrapmrnadecayproductsimplicationsforthelocalizationofmrnasbyms2mcpsystem

Ejemplares similares