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Genomic Characteristics of Chinese Borrelia burgdorferi Isolates

In China, B. burgdorferi, B.garinii, B. afzelii and B. yangtze sp. nov have been reported; B.garinii and B. afzelii are the main pathogenic genotypes. But until now only one Chinese strain was reported with whole genome sequence. In order to further understand the genomic characteristics and diversi...

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Autores principales: Hao, Qin, Du, Pengcheng, Zhang, Wen, Hou, Xuexia, Zhang, Lin, Zhang, Yuanyuan, Liu, Huixin, Liu, Wei, Chen, Chen, Wan, Kanglin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836705/
https://www.ncbi.nlm.nih.gov/pubmed/27093540
http://dx.doi.org/10.1371/journal.pone.0153149
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author Hao, Qin
Du, Pengcheng
Zhang, Wen
Hou, Xuexia
Zhang, Lin
Zhang, Yuanyuan
Liu, Huixin
Liu, Wei
Chen, Chen
Wan, Kanglin
author_facet Hao, Qin
Du, Pengcheng
Zhang, Wen
Hou, Xuexia
Zhang, Lin
Zhang, Yuanyuan
Liu, Huixin
Liu, Wei
Chen, Chen
Wan, Kanglin
author_sort Hao, Qin
collection PubMed
description In China, B. burgdorferi, B.garinii, B. afzelii and B. yangtze sp. nov have been reported; B.garinii and B. afzelii are the main pathogenic genotypes. But until now only one Chinese strain was reported with whole genome sequence. In order to further understand the genomic characteristics and diversity of Chinese Borrelia strains, 5 isolates from China were sequenced and compared with the whole genome sequences of strains in other areas. The results showed a high degree of conservation within the linear chromosome of Chinese strains, whereas plasmid showed a much larger diversity according to the majority genomic information of plasmids. The genome sequences of the five Chinese strains were compared with the corresponding reference strains, respectively, according to the genospecies. Pairwise analysis demonstrates that there are only 70 SNPs between the genomes of CS4 and B31. However, there are many more SNPs between the genomes of QX-S13 and VS116, PD91 and PBi, FP1 and PKo, R9 and Pko, respectively. Gene comparison showed some important different genes. OspA was one of the important different genes. Comparative genomic studies have found that OspA gene sequences of PD91 and R9 had great differences compared with the sequence of B31. OspA gene sequence of R9 had a 96bp deletion; OspA gene of PD91 had two deletions: 9bp and 10 bp. To conclude, we showed the genomic characteristics of four genotype Chinese B. burgdorferi strains. The genomic sequence of B. yangtze sp. nov and differences from B. valaisiana were first reported. Comparative analysis of Chinese strains with the different Borrelia species from other areas will help us to understand evolution and pathogenesis of Chinese Borrelia burgdorferi strains.
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spelling pubmed-48367052016-04-29 Genomic Characteristics of Chinese Borrelia burgdorferi Isolates Hao, Qin Du, Pengcheng Zhang, Wen Hou, Xuexia Zhang, Lin Zhang, Yuanyuan Liu, Huixin Liu, Wei Chen, Chen Wan, Kanglin PLoS One Research Article In China, B. burgdorferi, B.garinii, B. afzelii and B. yangtze sp. nov have been reported; B.garinii and B. afzelii are the main pathogenic genotypes. But until now only one Chinese strain was reported with whole genome sequence. In order to further understand the genomic characteristics and diversity of Chinese Borrelia strains, 5 isolates from China were sequenced and compared with the whole genome sequences of strains in other areas. The results showed a high degree of conservation within the linear chromosome of Chinese strains, whereas plasmid showed a much larger diversity according to the majority genomic information of plasmids. The genome sequences of the five Chinese strains were compared with the corresponding reference strains, respectively, according to the genospecies. Pairwise analysis demonstrates that there are only 70 SNPs between the genomes of CS4 and B31. However, there are many more SNPs between the genomes of QX-S13 and VS116, PD91 and PBi, FP1 and PKo, R9 and Pko, respectively. Gene comparison showed some important different genes. OspA was one of the important different genes. Comparative genomic studies have found that OspA gene sequences of PD91 and R9 had great differences compared with the sequence of B31. OspA gene sequence of R9 had a 96bp deletion; OspA gene of PD91 had two deletions: 9bp and 10 bp. To conclude, we showed the genomic characteristics of four genotype Chinese B. burgdorferi strains. The genomic sequence of B. yangtze sp. nov and differences from B. valaisiana were first reported. Comparative analysis of Chinese strains with the different Borrelia species from other areas will help us to understand evolution and pathogenesis of Chinese Borrelia burgdorferi strains. Public Library of Science 2016-04-19 /pmc/articles/PMC4836705/ /pubmed/27093540 http://dx.doi.org/10.1371/journal.pone.0153149 Text en © 2016 Hao et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Hao, Qin
Du, Pengcheng
Zhang, Wen
Hou, Xuexia
Zhang, Lin
Zhang, Yuanyuan
Liu, Huixin
Liu, Wei
Chen, Chen
Wan, Kanglin
Genomic Characteristics of Chinese Borrelia burgdorferi Isolates
title Genomic Characteristics of Chinese Borrelia burgdorferi Isolates
title_full Genomic Characteristics of Chinese Borrelia burgdorferi Isolates
title_fullStr Genomic Characteristics of Chinese Borrelia burgdorferi Isolates
title_full_unstemmed Genomic Characteristics of Chinese Borrelia burgdorferi Isolates
title_short Genomic Characteristics of Chinese Borrelia burgdorferi Isolates
title_sort genomic characteristics of chinese borrelia burgdorferi isolates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836705/
https://www.ncbi.nlm.nih.gov/pubmed/27093540
http://dx.doi.org/10.1371/journal.pone.0153149
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