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Association between Polycystic Ovary Syndrome and Gut Microbiota

Polycystic ovary syndrome (PCOS) is the most frequent endocrinopathy in women of reproductive age. It is difficult to treat PCOS because of its complex etiology and pathogenesis. Here, we characterized the roles of gut microbiota on the pathogenesis and treatments in letrozole (a nonsteroidal aromat...

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Autores principales: Guo, Yanjie, Qi, Yane, Yang, Xuefei, Zhao, Lihui, Wen, Shu, Liu, Yinhui, Tang, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836746/
https://www.ncbi.nlm.nih.gov/pubmed/27093642
http://dx.doi.org/10.1371/journal.pone.0153196
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author Guo, Yanjie
Qi, Yane
Yang, Xuefei
Zhao, Lihui
Wen, Shu
Liu, Yinhui
Tang, Li
author_facet Guo, Yanjie
Qi, Yane
Yang, Xuefei
Zhao, Lihui
Wen, Shu
Liu, Yinhui
Tang, Li
author_sort Guo, Yanjie
collection PubMed
description Polycystic ovary syndrome (PCOS) is the most frequent endocrinopathy in women of reproductive age. It is difficult to treat PCOS because of its complex etiology and pathogenesis. Here, we characterized the roles of gut microbiota on the pathogenesis and treatments in letrozole (a nonsteroidal aromatase inhibitor) induced PCOS rat model. Changes in estrous cycles, hormonal levels, ovarian morphology and gut microbiota by PCR-DGGE and real-time PCR were determined. The results showed that PCOS rats displayed abnormal estrous cycles with increasing androgen biosynthesis and exhibited multiple large cysts with diminished granulosa layers in ovarian tissues. Meanwhile, the composition of gut microbiota in letrozole-treated rats was different from that in the controls. Lactobacillus, Ruminococcus and Clostridium were lower while Prevotella was higher in PCOS rats when compared with control rats. After treating PCOS rats with Lactobacillus and fecal microbiota transplantation (FMT) from healthy rats, it was found that the estrous cycles were improved in all 8 rats in FMT group, and in 6 of the 8 rats in Lactobacillus transplantation group with decreasing androgen biosynthesis. Their ovarian morphologies normalized. The composition of gut microbiota restored in both FMT and Lactobacillus treated groups with increasing of Lactobacillus and Clostridium, and decreasing of Prevotella. These results indicated that dysbiosis of gut microbiota was associated with the pathogenesis of PCOS. Microbiota interventions through FMT and Lactobacillus transplantation were beneficial for the treatments of PCOS rats.
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spelling pubmed-48367462016-04-29 Association between Polycystic Ovary Syndrome and Gut Microbiota Guo, Yanjie Qi, Yane Yang, Xuefei Zhao, Lihui Wen, Shu Liu, Yinhui Tang, Li PLoS One Research Article Polycystic ovary syndrome (PCOS) is the most frequent endocrinopathy in women of reproductive age. It is difficult to treat PCOS because of its complex etiology and pathogenesis. Here, we characterized the roles of gut microbiota on the pathogenesis and treatments in letrozole (a nonsteroidal aromatase inhibitor) induced PCOS rat model. Changes in estrous cycles, hormonal levels, ovarian morphology and gut microbiota by PCR-DGGE and real-time PCR were determined. The results showed that PCOS rats displayed abnormal estrous cycles with increasing androgen biosynthesis and exhibited multiple large cysts with diminished granulosa layers in ovarian tissues. Meanwhile, the composition of gut microbiota in letrozole-treated rats was different from that in the controls. Lactobacillus, Ruminococcus and Clostridium were lower while Prevotella was higher in PCOS rats when compared with control rats. After treating PCOS rats with Lactobacillus and fecal microbiota transplantation (FMT) from healthy rats, it was found that the estrous cycles were improved in all 8 rats in FMT group, and in 6 of the 8 rats in Lactobacillus transplantation group with decreasing androgen biosynthesis. Their ovarian morphologies normalized. The composition of gut microbiota restored in both FMT and Lactobacillus treated groups with increasing of Lactobacillus and Clostridium, and decreasing of Prevotella. These results indicated that dysbiosis of gut microbiota was associated with the pathogenesis of PCOS. Microbiota interventions through FMT and Lactobacillus transplantation were beneficial for the treatments of PCOS rats. Public Library of Science 2016-04-19 /pmc/articles/PMC4836746/ /pubmed/27093642 http://dx.doi.org/10.1371/journal.pone.0153196 Text en © 2016 Guo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Guo, Yanjie
Qi, Yane
Yang, Xuefei
Zhao, Lihui
Wen, Shu
Liu, Yinhui
Tang, Li
Association between Polycystic Ovary Syndrome and Gut Microbiota
title Association between Polycystic Ovary Syndrome and Gut Microbiota
title_full Association between Polycystic Ovary Syndrome and Gut Microbiota
title_fullStr Association between Polycystic Ovary Syndrome and Gut Microbiota
title_full_unstemmed Association between Polycystic Ovary Syndrome and Gut Microbiota
title_short Association between Polycystic Ovary Syndrome and Gut Microbiota
title_sort association between polycystic ovary syndrome and gut microbiota
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836746/
https://www.ncbi.nlm.nih.gov/pubmed/27093642
http://dx.doi.org/10.1371/journal.pone.0153196
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