Municipal Solid Waste Landfills Harbor Distinct Microbiomes

Landfills are the final repository for most of the discarded material from human society and its “built environments.” Microorganisms subsequently degrade this discarded material in the landfill, releasing gases (largely CH(4) and CO(2)) and a complex mixture of soluble chemical compounds in leachat...

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Autores principales: Stamps, Blake W., Lyles, Christopher N., Suflita, Joseph M., Masoner, Jason R., Cozzarelli, Isabelle M., Kolpin, Dana W., Stevenson, Bradley S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4837139/
https://www.ncbi.nlm.nih.gov/pubmed/27148222
http://dx.doi.org/10.3389/fmicb.2016.00534
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author Stamps, Blake W.
Lyles, Christopher N.
Suflita, Joseph M.
Masoner, Jason R.
Cozzarelli, Isabelle M.
Kolpin, Dana W.
Stevenson, Bradley S.
author_facet Stamps, Blake W.
Lyles, Christopher N.
Suflita, Joseph M.
Masoner, Jason R.
Cozzarelli, Isabelle M.
Kolpin, Dana W.
Stevenson, Bradley S.
author_sort Stamps, Blake W.
collection PubMed
description Landfills are the final repository for most of the discarded material from human society and its “built environments.” Microorganisms subsequently degrade this discarded material in the landfill, releasing gases (largely CH(4) and CO(2)) and a complex mixture of soluble chemical compounds in leachate. Characterization of “landfill microbiomes” and their comparison across several landfills should allow the identification of environmental or operational properties that influence the composition of these microbiomes and potentially their biodegradation capabilities. To this end, the composition of landfill microbiomes was characterized as part of an ongoing USGS national survey studying the chemical composition of leachates from 19 non-hazardous landfills across 16 states in the continental U.S. The landfills varied in parameters such as size, waste composition, management strategy, geography, and climate zone. The diversity and composition of bacterial and archaeal populations in leachate samples were characterized by 16S rRNA gene sequence analysis, and compared against a variety of physical and chemical parameters in an attempt to identify their impact on selection. Members of the Epsilonproteobacteria, Gammaproteobacteria, Clostridia, and candidate division OP3 were the most abundant. The distribution of the observed phylogenetic diversity could best be explained by a combination of variables and was correlated most strongly with the concentrations of chloride and barium, rate of evapotranspiration, age of waste, and the number of detected household chemicals. This study illustrates how leachate microbiomes are distinct from those of other natural or built environments, and sheds light on the major selective forces responsible for this microbial diversity.
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spelling pubmed-48371392016-05-04 Municipal Solid Waste Landfills Harbor Distinct Microbiomes Stamps, Blake W. Lyles, Christopher N. Suflita, Joseph M. Masoner, Jason R. Cozzarelli, Isabelle M. Kolpin, Dana W. Stevenson, Bradley S. Front Microbiol Microbiology Landfills are the final repository for most of the discarded material from human society and its “built environments.” Microorganisms subsequently degrade this discarded material in the landfill, releasing gases (largely CH(4) and CO(2)) and a complex mixture of soluble chemical compounds in leachate. Characterization of “landfill microbiomes” and their comparison across several landfills should allow the identification of environmental or operational properties that influence the composition of these microbiomes and potentially their biodegradation capabilities. To this end, the composition of landfill microbiomes was characterized as part of an ongoing USGS national survey studying the chemical composition of leachates from 19 non-hazardous landfills across 16 states in the continental U.S. The landfills varied in parameters such as size, waste composition, management strategy, geography, and climate zone. The diversity and composition of bacterial and archaeal populations in leachate samples were characterized by 16S rRNA gene sequence analysis, and compared against a variety of physical and chemical parameters in an attempt to identify their impact on selection. Members of the Epsilonproteobacteria, Gammaproteobacteria, Clostridia, and candidate division OP3 were the most abundant. The distribution of the observed phylogenetic diversity could best be explained by a combination of variables and was correlated most strongly with the concentrations of chloride and barium, rate of evapotranspiration, age of waste, and the number of detected household chemicals. This study illustrates how leachate microbiomes are distinct from those of other natural or built environments, and sheds light on the major selective forces responsible for this microbial diversity. Frontiers Media S.A. 2016-04-20 /pmc/articles/PMC4837139/ /pubmed/27148222 http://dx.doi.org/10.3389/fmicb.2016.00534 Text en Copyright © 2016 Stamps, Lyles, Suflita, Masoner, Cozzarelli, Kolpin and Stevenson. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Stamps, Blake W.
Lyles, Christopher N.
Suflita, Joseph M.
Masoner, Jason R.
Cozzarelli, Isabelle M.
Kolpin, Dana W.
Stevenson, Bradley S.
Municipal Solid Waste Landfills Harbor Distinct Microbiomes
title Municipal Solid Waste Landfills Harbor Distinct Microbiomes
title_full Municipal Solid Waste Landfills Harbor Distinct Microbiomes
title_fullStr Municipal Solid Waste Landfills Harbor Distinct Microbiomes
title_full_unstemmed Municipal Solid Waste Landfills Harbor Distinct Microbiomes
title_short Municipal Solid Waste Landfills Harbor Distinct Microbiomes
title_sort municipal solid waste landfills harbor distinct microbiomes
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4837139/
https://www.ncbi.nlm.nih.gov/pubmed/27148222
http://dx.doi.org/10.3389/fmicb.2016.00534
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