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Genomic and immunologic factors associated with viral pathogenesis in a lethal EV71 infected neonatal mouse model

Hand, foot and mouth disease (HFMD) caused by enterovirus 71 (EV71) has emerged as a major health problem in China and worldwide. The present study aimed to understand the virological features of EV71 and host responses resulting from EV71 infection. Six different EV71 strains were isolated from HFM...

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Autores principales: YUE, YINGYING, LI, PENG, SONG, NANNAN, LI, BINGQING, LI, ZHIHUI, GUO, YUQI, ZHANG, WEIDONG, WEI, MING Q., GAI, ZHONGTAO, MENG, HONG, WANG, JIWEN, QIN, LIZENG
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838153/
https://www.ncbi.nlm.nih.gov/pubmed/27035332
http://dx.doi.org/10.3892/mmr.2016.5080
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author YUE, YINGYING
LI, PENG
SONG, NANNAN
LI, BINGQING
LI, ZHIHUI
GUO, YUQI
ZHANG, WEIDONG
WEI, MING Q.
GAI, ZHONGTAO
MENG, HONG
WANG, JIWEN
QIN, LIZENG
author_facet YUE, YINGYING
LI, PENG
SONG, NANNAN
LI, BINGQING
LI, ZHIHUI
GUO, YUQI
ZHANG, WEIDONG
WEI, MING Q.
GAI, ZHONGTAO
MENG, HONG
WANG, JIWEN
QIN, LIZENG
author_sort YUE, YINGYING
collection PubMed
description Hand, foot and mouth disease (HFMD) caused by enterovirus 71 (EV71) has emerged as a major health problem in China and worldwide. The present study aimed to understand the virological features of EV71 and host responses resulting from EV71 infection. Six different EV71 strains were isolated from HFMD patients with severe or mild clinical symptoms, and were analyzed for pathogenicity in vitro and in vivo. The results demonstrated that the six virus strains exhibited similar cytopathogenic effects on susceptible MA104 cells. However, marked differences in histological and immunopathological changes were observed when mice were inoculated with the different virus strains. Thus, the viruses studied were divided into two groups, highly or weakly pathogenic. Two representative virus strains, JN200804 and JN200803 (highly and weakly pathogenic, respectively) were studied further to investigate pathogenicity-associated factors, including genetic mutations and immunopathogenesis. The present study has demonstrated that highly pathogenic strains have stable genome and amino acid sequences. Notably, the present study demonstrated that a highly pathogenic strain induced a significant increase of the bulk CD4 T cell levels at 3 days post-inoculation. In conclusion, the current study demonstrates that genomic and immunologic factors may be responsible for the multiple tissue damage caused by highly pathogenic EV71 infection.
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spelling pubmed-48381532016-04-21 Genomic and immunologic factors associated with viral pathogenesis in a lethal EV71 infected neonatal mouse model YUE, YINGYING LI, PENG SONG, NANNAN LI, BINGQING LI, ZHIHUI GUO, YUQI ZHANG, WEIDONG WEI, MING Q. GAI, ZHONGTAO MENG, HONG WANG, JIWEN QIN, LIZENG Mol Med Rep Articles Hand, foot and mouth disease (HFMD) caused by enterovirus 71 (EV71) has emerged as a major health problem in China and worldwide. The present study aimed to understand the virological features of EV71 and host responses resulting from EV71 infection. Six different EV71 strains were isolated from HFMD patients with severe or mild clinical symptoms, and were analyzed for pathogenicity in vitro and in vivo. The results demonstrated that the six virus strains exhibited similar cytopathogenic effects on susceptible MA104 cells. However, marked differences in histological and immunopathological changes were observed when mice were inoculated with the different virus strains. Thus, the viruses studied were divided into two groups, highly or weakly pathogenic. Two representative virus strains, JN200804 and JN200803 (highly and weakly pathogenic, respectively) were studied further to investigate pathogenicity-associated factors, including genetic mutations and immunopathogenesis. The present study has demonstrated that highly pathogenic strains have stable genome and amino acid sequences. Notably, the present study demonstrated that a highly pathogenic strain induced a significant increase of the bulk CD4 T cell levels at 3 days post-inoculation. In conclusion, the current study demonstrates that genomic and immunologic factors may be responsible for the multiple tissue damage caused by highly pathogenic EV71 infection. D.A. Spandidos 2016-05 2016-03-31 /pmc/articles/PMC4838153/ /pubmed/27035332 http://dx.doi.org/10.3892/mmr.2016.5080 Text en Copyright: © Yue et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
YUE, YINGYING
LI, PENG
SONG, NANNAN
LI, BINGQING
LI, ZHIHUI
GUO, YUQI
ZHANG, WEIDONG
WEI, MING Q.
GAI, ZHONGTAO
MENG, HONG
WANG, JIWEN
QIN, LIZENG
Genomic and immunologic factors associated with viral pathogenesis in a lethal EV71 infected neonatal mouse model
title Genomic and immunologic factors associated with viral pathogenesis in a lethal EV71 infected neonatal mouse model
title_full Genomic and immunologic factors associated with viral pathogenesis in a lethal EV71 infected neonatal mouse model
title_fullStr Genomic and immunologic factors associated with viral pathogenesis in a lethal EV71 infected neonatal mouse model
title_full_unstemmed Genomic and immunologic factors associated with viral pathogenesis in a lethal EV71 infected neonatal mouse model
title_short Genomic and immunologic factors associated with viral pathogenesis in a lethal EV71 infected neonatal mouse model
title_sort genomic and immunologic factors associated with viral pathogenesis in a lethal ev71 infected neonatal mouse model
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838153/
https://www.ncbi.nlm.nih.gov/pubmed/27035332
http://dx.doi.org/10.3892/mmr.2016.5080
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