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Lymphocyte Redox Imbalance and Reduced Proliferation after a Single Session of High Intensity Interval Exercise
This study investigated whether an acute session of high-intensity interval training (HIIT) is sufficient to alter lymphocyte function and redox status. Sixteen young healthy men underwent a HIIT session on a cycloergometer, consisting of eight bouts of 1 min at 90–100% of peak power, with 75 second...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838252/ https://www.ncbi.nlm.nih.gov/pubmed/27096389 http://dx.doi.org/10.1371/journal.pone.0153647 |
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author | Tossige-Gomes, Rosalina Costa, Karine Beatriz Ottone, Vinícius de Oliveira Magalhães, Flávio de Castro Amorim, Fabiano Trigueiro Rocha-Vieira, Etel |
author_facet | Tossige-Gomes, Rosalina Costa, Karine Beatriz Ottone, Vinícius de Oliveira Magalhães, Flávio de Castro Amorim, Fabiano Trigueiro Rocha-Vieira, Etel |
author_sort | Tossige-Gomes, Rosalina |
collection | PubMed |
description | This study investigated whether an acute session of high-intensity interval training (HIIT) is sufficient to alter lymphocyte function and redox status. Sixteen young healthy men underwent a HIIT session on a cycloergometer, consisting of eight bouts of 1 min at 90–100% of peak power, with 75 seconds of active recovery at 30 W between bouts. Venous blood was collected before, immediately after, and 30 minutes after the HIIT session. In response to Staphylococcus aureus superantigen B (SEB) stimulation, lymphocyte proliferation decreased and the IL-2 concentration increased after the HIIT session. However, the HIIT session had no effect on lymphocyte proliferation or IL-2 response to phytohemagglutinin stimulation. The HIIT session also induced lymphocyte redox imbalance, characterized by an increase in the concentration of thiobarbituric acid reactive substances and a decrease in the activity of the antioxidant enzyme catalase. Lymphocyte viability was not affected by the HIIT session. The frequencies of CD25(+) and CD69(+) T helper and B lymphocytes in response to superantigen stimulation were lower after exercise, suggesting that superantigen-induced lymphocyte activation was reduced by HIIT. However, HIIT also led to a reduction in the frequency of CD4(+) and CD19(+) cells, so the frequencies of CD25(+) and CD69(+) cells within the CD4 and CD19 cell populations were not affected by HIIT. These data indicate that the reduced lymphocyte proliferation observed after HIIT is not due to reduced early lymphocyte activation by superantigen. Our findings show that an acute HIIT session promotes lymphocyte redox imbalance and reduces lymphocyte proliferation in response to superantigenic, but not to mitogenic stimulation. This observation cannot be explained by alteration of the early lymphocyte activation response to superantigen. The manner in which lymphocyte function modulation by an acute HIIT session can affect individual immunity and susceptibility to infection is important and requires further investigation. |
format | Online Article Text |
id | pubmed-4838252 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-48382522016-04-29 Lymphocyte Redox Imbalance and Reduced Proliferation after a Single Session of High Intensity Interval Exercise Tossige-Gomes, Rosalina Costa, Karine Beatriz Ottone, Vinícius de Oliveira Magalhães, Flávio de Castro Amorim, Fabiano Trigueiro Rocha-Vieira, Etel PLoS One Research Article This study investigated whether an acute session of high-intensity interval training (HIIT) is sufficient to alter lymphocyte function and redox status. Sixteen young healthy men underwent a HIIT session on a cycloergometer, consisting of eight bouts of 1 min at 90–100% of peak power, with 75 seconds of active recovery at 30 W between bouts. Venous blood was collected before, immediately after, and 30 minutes after the HIIT session. In response to Staphylococcus aureus superantigen B (SEB) stimulation, lymphocyte proliferation decreased and the IL-2 concentration increased after the HIIT session. However, the HIIT session had no effect on lymphocyte proliferation or IL-2 response to phytohemagglutinin stimulation. The HIIT session also induced lymphocyte redox imbalance, characterized by an increase in the concentration of thiobarbituric acid reactive substances and a decrease in the activity of the antioxidant enzyme catalase. Lymphocyte viability was not affected by the HIIT session. The frequencies of CD25(+) and CD69(+) T helper and B lymphocytes in response to superantigen stimulation were lower after exercise, suggesting that superantigen-induced lymphocyte activation was reduced by HIIT. However, HIIT also led to a reduction in the frequency of CD4(+) and CD19(+) cells, so the frequencies of CD25(+) and CD69(+) cells within the CD4 and CD19 cell populations were not affected by HIIT. These data indicate that the reduced lymphocyte proliferation observed after HIIT is not due to reduced early lymphocyte activation by superantigen. Our findings show that an acute HIIT session promotes lymphocyte redox imbalance and reduces lymphocyte proliferation in response to superantigenic, but not to mitogenic stimulation. This observation cannot be explained by alteration of the early lymphocyte activation response to superantigen. The manner in which lymphocyte function modulation by an acute HIIT session can affect individual immunity and susceptibility to infection is important and requires further investigation. Public Library of Science 2016-04-20 /pmc/articles/PMC4838252/ /pubmed/27096389 http://dx.doi.org/10.1371/journal.pone.0153647 Text en © 2016 Tossige-Gomes et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Tossige-Gomes, Rosalina Costa, Karine Beatriz Ottone, Vinícius de Oliveira Magalhães, Flávio de Castro Amorim, Fabiano Trigueiro Rocha-Vieira, Etel Lymphocyte Redox Imbalance and Reduced Proliferation after a Single Session of High Intensity Interval Exercise |
title | Lymphocyte Redox Imbalance and Reduced Proliferation after a Single Session of High Intensity Interval Exercise |
title_full | Lymphocyte Redox Imbalance and Reduced Proliferation after a Single Session of High Intensity Interval Exercise |
title_fullStr | Lymphocyte Redox Imbalance and Reduced Proliferation after a Single Session of High Intensity Interval Exercise |
title_full_unstemmed | Lymphocyte Redox Imbalance and Reduced Proliferation after a Single Session of High Intensity Interval Exercise |
title_short | Lymphocyte Redox Imbalance and Reduced Proliferation after a Single Session of High Intensity Interval Exercise |
title_sort | lymphocyte redox imbalance and reduced proliferation after a single session of high intensity interval exercise |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838252/ https://www.ncbi.nlm.nih.gov/pubmed/27096389 http://dx.doi.org/10.1371/journal.pone.0153647 |
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