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Lymphocyte Redox Imbalance and Reduced Proliferation after a Single Session of High Intensity Interval Exercise

This study investigated whether an acute session of high-intensity interval training (HIIT) is sufficient to alter lymphocyte function and redox status. Sixteen young healthy men underwent a HIIT session on a cycloergometer, consisting of eight bouts of 1 min at 90–100% of peak power, with 75 second...

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Autores principales: Tossige-Gomes, Rosalina, Costa, Karine Beatriz, Ottone, Vinícius de Oliveira, Magalhães, Flávio de Castro, Amorim, Fabiano Trigueiro, Rocha-Vieira, Etel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838252/
https://www.ncbi.nlm.nih.gov/pubmed/27096389
http://dx.doi.org/10.1371/journal.pone.0153647
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author Tossige-Gomes, Rosalina
Costa, Karine Beatriz
Ottone, Vinícius de Oliveira
Magalhães, Flávio de Castro
Amorim, Fabiano Trigueiro
Rocha-Vieira, Etel
author_facet Tossige-Gomes, Rosalina
Costa, Karine Beatriz
Ottone, Vinícius de Oliveira
Magalhães, Flávio de Castro
Amorim, Fabiano Trigueiro
Rocha-Vieira, Etel
author_sort Tossige-Gomes, Rosalina
collection PubMed
description This study investigated whether an acute session of high-intensity interval training (HIIT) is sufficient to alter lymphocyte function and redox status. Sixteen young healthy men underwent a HIIT session on a cycloergometer, consisting of eight bouts of 1 min at 90–100% of peak power, with 75 seconds of active recovery at 30 W between bouts. Venous blood was collected before, immediately after, and 30 minutes after the HIIT session. In response to Staphylococcus aureus superantigen B (SEB) stimulation, lymphocyte proliferation decreased and the IL-2 concentration increased after the HIIT session. However, the HIIT session had no effect on lymphocyte proliferation or IL-2 response to phytohemagglutinin stimulation. The HIIT session also induced lymphocyte redox imbalance, characterized by an increase in the concentration of thiobarbituric acid reactive substances and a decrease in the activity of the antioxidant enzyme catalase. Lymphocyte viability was not affected by the HIIT session. The frequencies of CD25(+) and CD69(+) T helper and B lymphocytes in response to superantigen stimulation were lower after exercise, suggesting that superantigen-induced lymphocyte activation was reduced by HIIT. However, HIIT also led to a reduction in the frequency of CD4(+) and CD19(+) cells, so the frequencies of CD25(+) and CD69(+) cells within the CD4 and CD19 cell populations were not affected by HIIT. These data indicate that the reduced lymphocyte proliferation observed after HIIT is not due to reduced early lymphocyte activation by superantigen. Our findings show that an acute HIIT session promotes lymphocyte redox imbalance and reduces lymphocyte proliferation in response to superantigenic, but not to mitogenic stimulation. This observation cannot be explained by alteration of the early lymphocyte activation response to superantigen. The manner in which lymphocyte function modulation by an acute HIIT session can affect individual immunity and susceptibility to infection is important and requires further investigation.
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spelling pubmed-48382522016-04-29 Lymphocyte Redox Imbalance and Reduced Proliferation after a Single Session of High Intensity Interval Exercise Tossige-Gomes, Rosalina Costa, Karine Beatriz Ottone, Vinícius de Oliveira Magalhães, Flávio de Castro Amorim, Fabiano Trigueiro Rocha-Vieira, Etel PLoS One Research Article This study investigated whether an acute session of high-intensity interval training (HIIT) is sufficient to alter lymphocyte function and redox status. Sixteen young healthy men underwent a HIIT session on a cycloergometer, consisting of eight bouts of 1 min at 90–100% of peak power, with 75 seconds of active recovery at 30 W between bouts. Venous blood was collected before, immediately after, and 30 minutes after the HIIT session. In response to Staphylococcus aureus superantigen B (SEB) stimulation, lymphocyte proliferation decreased and the IL-2 concentration increased after the HIIT session. However, the HIIT session had no effect on lymphocyte proliferation or IL-2 response to phytohemagglutinin stimulation. The HIIT session also induced lymphocyte redox imbalance, characterized by an increase in the concentration of thiobarbituric acid reactive substances and a decrease in the activity of the antioxidant enzyme catalase. Lymphocyte viability was not affected by the HIIT session. The frequencies of CD25(+) and CD69(+) T helper and B lymphocytes in response to superantigen stimulation were lower after exercise, suggesting that superantigen-induced lymphocyte activation was reduced by HIIT. However, HIIT also led to a reduction in the frequency of CD4(+) and CD19(+) cells, so the frequencies of CD25(+) and CD69(+) cells within the CD4 and CD19 cell populations were not affected by HIIT. These data indicate that the reduced lymphocyte proliferation observed after HIIT is not due to reduced early lymphocyte activation by superantigen. Our findings show that an acute HIIT session promotes lymphocyte redox imbalance and reduces lymphocyte proliferation in response to superantigenic, but not to mitogenic stimulation. This observation cannot be explained by alteration of the early lymphocyte activation response to superantigen. The manner in which lymphocyte function modulation by an acute HIIT session can affect individual immunity and susceptibility to infection is important and requires further investigation. Public Library of Science 2016-04-20 /pmc/articles/PMC4838252/ /pubmed/27096389 http://dx.doi.org/10.1371/journal.pone.0153647 Text en © 2016 Tossige-Gomes et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Tossige-Gomes, Rosalina
Costa, Karine Beatriz
Ottone, Vinícius de Oliveira
Magalhães, Flávio de Castro
Amorim, Fabiano Trigueiro
Rocha-Vieira, Etel
Lymphocyte Redox Imbalance and Reduced Proliferation after a Single Session of High Intensity Interval Exercise
title Lymphocyte Redox Imbalance and Reduced Proliferation after a Single Session of High Intensity Interval Exercise
title_full Lymphocyte Redox Imbalance and Reduced Proliferation after a Single Session of High Intensity Interval Exercise
title_fullStr Lymphocyte Redox Imbalance and Reduced Proliferation after a Single Session of High Intensity Interval Exercise
title_full_unstemmed Lymphocyte Redox Imbalance and Reduced Proliferation after a Single Session of High Intensity Interval Exercise
title_short Lymphocyte Redox Imbalance and Reduced Proliferation after a Single Session of High Intensity Interval Exercise
title_sort lymphocyte redox imbalance and reduced proliferation after a single session of high intensity interval exercise
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838252/
https://www.ncbi.nlm.nih.gov/pubmed/27096389
http://dx.doi.org/10.1371/journal.pone.0153647
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