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Osteoclastogenesis in Local Alveolar Bone in Early Decortication-Facilitated Orthodontic Tooth Movement

OBJECTIVE: In the current study, we aimed to investigate the effects of alveolar decortication on local bone remodeling, and to explore the possible mechanism by which decortication facilitates tooth movement. MATERIALS AND METHODS: Forty rabbits were included in the experiment. The left mandible wa...

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Autores principales: Chen, Ya-Wen, Wang, Hai-Cheng, Gao, Long-Hua, Liu, Chang, Jiang, Yu-Xi, Qu, Hong, Li, Cui-Ying, Jiang, Jiu-Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838268/
https://www.ncbi.nlm.nih.gov/pubmed/27096621
http://dx.doi.org/10.1371/journal.pone.0153937
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author Chen, Ya-Wen
Wang, Hai-Cheng
Gao, Long-Hua
Liu, Chang
Jiang, Yu-Xi
Qu, Hong
Li, Cui-Ying
Jiang, Jiu-Hui
author_facet Chen, Ya-Wen
Wang, Hai-Cheng
Gao, Long-Hua
Liu, Chang
Jiang, Yu-Xi
Qu, Hong
Li, Cui-Ying
Jiang, Jiu-Hui
author_sort Chen, Ya-Wen
collection PubMed
description OBJECTIVE: In the current study, we aimed to investigate the effects of alveolar decortication on local bone remodeling, and to explore the possible mechanism by which decortication facilitates tooth movement. MATERIALS AND METHODS: Forty rabbits were included in the experiment. The left mandible was subjected to decortication-facilitated orthodontics, and the right mandible underwent traditional orthodontics as a control. The animals were sacrificed on the days 1, 3, 5, 7 and 14, after undergoing orthodontic procedures. Tooth movement was measured by Micro-CT, and the local periodontal tissues were investigated using H&E, Masson's trichrome and tartrate-resistant acid phosphatase (TRAP) staining. The mRNA levels of genes related to bone remodeling in the alveolar bone were analyzed using real-time PCR. RESULT: On days 3, 5, 7 and 14, tooth movement was statistically accelerated by decortication (P < 0.05) and was accompanied by increased hyperemia. Despite the lack of new bone formation in both groups, more osteoclasts were noted in the decorticated group, with two peak counts (P < 0.05). The first peak count was consistent with the maximum values of ctsk and TRAP expression, and the second peak counts accompanied the maximum nfatc1 and jdp2 expression. The increased fra2 expression and the ratio of rankl/opg also accompanied the second peak counts. CONCLUSIONS: Following alveolar decortication, osteoclastogenesis was initially induced to a greater degree than the new bone formation which was thought to have caused a regional acceleratory phenomenon (RAP). The amount of steoclastogenesis in the decorticated alveolar bone was found to have two peaks, perhaps due to attenuated local resistance. The first peak count in osteoclasts may have been due to previously existing osteoclast precursors, whereas the second may represent the differentiation of peripheral blood mononuclear cells which came from circulation as the result of hyperemia.
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spelling pubmed-48382682016-04-29 Osteoclastogenesis in Local Alveolar Bone in Early Decortication-Facilitated Orthodontic Tooth Movement Chen, Ya-Wen Wang, Hai-Cheng Gao, Long-Hua Liu, Chang Jiang, Yu-Xi Qu, Hong Li, Cui-Ying Jiang, Jiu-Hui PLoS One Research Article OBJECTIVE: In the current study, we aimed to investigate the effects of alveolar decortication on local bone remodeling, and to explore the possible mechanism by which decortication facilitates tooth movement. MATERIALS AND METHODS: Forty rabbits were included in the experiment. The left mandible was subjected to decortication-facilitated orthodontics, and the right mandible underwent traditional orthodontics as a control. The animals were sacrificed on the days 1, 3, 5, 7 and 14, after undergoing orthodontic procedures. Tooth movement was measured by Micro-CT, and the local periodontal tissues were investigated using H&E, Masson's trichrome and tartrate-resistant acid phosphatase (TRAP) staining. The mRNA levels of genes related to bone remodeling in the alveolar bone were analyzed using real-time PCR. RESULT: On days 3, 5, 7 and 14, tooth movement was statistically accelerated by decortication (P < 0.05) and was accompanied by increased hyperemia. Despite the lack of new bone formation in both groups, more osteoclasts were noted in the decorticated group, with two peak counts (P < 0.05). The first peak count was consistent with the maximum values of ctsk and TRAP expression, and the second peak counts accompanied the maximum nfatc1 and jdp2 expression. The increased fra2 expression and the ratio of rankl/opg also accompanied the second peak counts. CONCLUSIONS: Following alveolar decortication, osteoclastogenesis was initially induced to a greater degree than the new bone formation which was thought to have caused a regional acceleratory phenomenon (RAP). The amount of steoclastogenesis in the decorticated alveolar bone was found to have two peaks, perhaps due to attenuated local resistance. The first peak count in osteoclasts may have been due to previously existing osteoclast precursors, whereas the second may represent the differentiation of peripheral blood mononuclear cells which came from circulation as the result of hyperemia. Public Library of Science 2016-04-20 /pmc/articles/PMC4838268/ /pubmed/27096621 http://dx.doi.org/10.1371/journal.pone.0153937 Text en © 2016 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Chen, Ya-Wen
Wang, Hai-Cheng
Gao, Long-Hua
Liu, Chang
Jiang, Yu-Xi
Qu, Hong
Li, Cui-Ying
Jiang, Jiu-Hui
Osteoclastogenesis in Local Alveolar Bone in Early Decortication-Facilitated Orthodontic Tooth Movement
title Osteoclastogenesis in Local Alveolar Bone in Early Decortication-Facilitated Orthodontic Tooth Movement
title_full Osteoclastogenesis in Local Alveolar Bone in Early Decortication-Facilitated Orthodontic Tooth Movement
title_fullStr Osteoclastogenesis in Local Alveolar Bone in Early Decortication-Facilitated Orthodontic Tooth Movement
title_full_unstemmed Osteoclastogenesis in Local Alveolar Bone in Early Decortication-Facilitated Orthodontic Tooth Movement
title_short Osteoclastogenesis in Local Alveolar Bone in Early Decortication-Facilitated Orthodontic Tooth Movement
title_sort osteoclastogenesis in local alveolar bone in early decortication-facilitated orthodontic tooth movement
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838268/
https://www.ncbi.nlm.nih.gov/pubmed/27096621
http://dx.doi.org/10.1371/journal.pone.0153937
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