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Opportunities in the design and application of RNA for gene expression control

The past decade of synthetic biology research has witnessed numerous advances in the development of tools and frameworks for the design and characterization of biological systems. Researchers have focused on the use of RNA for gene expression control due to its versatility in sensing molecular ligan...

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Detalles Bibliográficos
Autores principales: McKeague, Maureen, Wong, Remus S., Smolke, Christina D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838379/
https://www.ncbi.nlm.nih.gov/pubmed/26969733
http://dx.doi.org/10.1093/nar/gkw151
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author McKeague, Maureen
Wong, Remus S.
Smolke, Christina D.
author_facet McKeague, Maureen
Wong, Remus S.
Smolke, Christina D.
author_sort McKeague, Maureen
collection PubMed
description The past decade of synthetic biology research has witnessed numerous advances in the development of tools and frameworks for the design and characterization of biological systems. Researchers have focused on the use of RNA for gene expression control due to its versatility in sensing molecular ligands and the relative ease by which RNA can be modeled and designed compared to proteins. We review the recent progress in the field with respect to RNA-based genetic devices that are controlled through small molecule and protein interactions. We discuss new approaches for generating and characterizing these devices and their underlying components. We also highlight immediate challenges, future directions and recent applications of synthetic RNA devices in engineered biological systems.
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spelling pubmed-48383792016-04-21 Opportunities in the design and application of RNA for gene expression control McKeague, Maureen Wong, Remus S. Smolke, Christina D. Nucleic Acids Res Survey and Summary The past decade of synthetic biology research has witnessed numerous advances in the development of tools and frameworks for the design and characterization of biological systems. Researchers have focused on the use of RNA for gene expression control due to its versatility in sensing molecular ligands and the relative ease by which RNA can be modeled and designed compared to proteins. We review the recent progress in the field with respect to RNA-based genetic devices that are controlled through small molecule and protein interactions. We discuss new approaches for generating and characterizing these devices and their underlying components. We also highlight immediate challenges, future directions and recent applications of synthetic RNA devices in engineered biological systems. Oxford University Press 2016-04-20 2016-03-11 /pmc/articles/PMC4838379/ /pubmed/26969733 http://dx.doi.org/10.1093/nar/gkw151 Text en © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Survey and Summary
McKeague, Maureen
Wong, Remus S.
Smolke, Christina D.
Opportunities in the design and application of RNA for gene expression control
title Opportunities in the design and application of RNA for gene expression control
title_full Opportunities in the design and application of RNA for gene expression control
title_fullStr Opportunities in the design and application of RNA for gene expression control
title_full_unstemmed Opportunities in the design and application of RNA for gene expression control
title_short Opportunities in the design and application of RNA for gene expression control
title_sort opportunities in the design and application of rna for gene expression control
topic Survey and Summary
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838379/
https://www.ncbi.nlm.nih.gov/pubmed/26969733
http://dx.doi.org/10.1093/nar/gkw151
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