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Pregnancy outcomes following the administration of high doses of dexamethasone in early pregnancy
OBJECTIVE: In the present study, we aimed to evaluate the effects of high doses of dexamethasone (DEX) in early pregnancy on pregnancy outcomes. METHODS: Pregnant BALB/c mice were treated with high-dose DEX in the experimental group or saline in the control group on gestational days (GDs) 0.5 to 4.5...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society for Reproductive Medicine
2016
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838577/ https://www.ncbi.nlm.nih.gov/pubmed/27104153 http://dx.doi.org/10.5653/cerm.2016.43.1.15 |
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author | Namdar Ahmadabad, Hasan Kayvan Jafari, Sabah Nezafat Firizi, Maryam Abbaspour, Ali Reza Ghafoori Gharib, Fahime Ghobadi, Yusef Gholizadeh, Samira |
author_facet | Namdar Ahmadabad, Hasan Kayvan Jafari, Sabah Nezafat Firizi, Maryam Abbaspour, Ali Reza Ghafoori Gharib, Fahime Ghobadi, Yusef Gholizadeh, Samira |
author_sort | Namdar Ahmadabad, Hasan |
collection | PubMed |
description | OBJECTIVE: In the present study, we aimed to evaluate the effects of high doses of dexamethasone (DEX) in early pregnancy on pregnancy outcomes. METHODS: Pregnant BALB/c mice were treated with high-dose DEX in the experimental group or saline in the control group on gestational days (GDs) 0.5 to 4.5. Pregnant mice were sacrificed on GDs 7.5, 13.5, or 18.5 and their peripheral blood, placentas, fetuses, and uterine tissue were collected. Decidual and placenta cell supernatants were examined to evaluate the effect of DEX on the proliferation of mononuclear cells, the quantity of uterine macrophages and uterine natural killer (uNK) cells, and levels of progesterone and 17β-estradiol, as determined by an 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay, immunohistochemistry, and enzyme-linked immunosorbent assay, respectively. We also were measured fetal and placental growth parameters on GD 18.5. RESULTS: We found that high doses of DEX were associated with an increased abortion rate, enhancement of the immunosuppressive effect of the decidua, alterations in placental growth parameters, decreased progesterone and 17β-estradiol levels, and a reduced frequency of macrophages and uNK cells. CONCLUSION: Our data suggest that the high-dose administration of DEX during early pregnancy negatively affected pregnancy outcomes. |
format | Online Article Text |
id | pubmed-4838577 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Korean Society for Reproductive Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-48385772016-04-21 Pregnancy outcomes following the administration of high doses of dexamethasone in early pregnancy Namdar Ahmadabad, Hasan Kayvan Jafari, Sabah Nezafat Firizi, Maryam Abbaspour, Ali Reza Ghafoori Gharib, Fahime Ghobadi, Yusef Gholizadeh, Samira Clin Exp Reprod Med Original Article OBJECTIVE: In the present study, we aimed to evaluate the effects of high doses of dexamethasone (DEX) in early pregnancy on pregnancy outcomes. METHODS: Pregnant BALB/c mice were treated with high-dose DEX in the experimental group or saline in the control group on gestational days (GDs) 0.5 to 4.5. Pregnant mice were sacrificed on GDs 7.5, 13.5, or 18.5 and their peripheral blood, placentas, fetuses, and uterine tissue were collected. Decidual and placenta cell supernatants were examined to evaluate the effect of DEX on the proliferation of mononuclear cells, the quantity of uterine macrophages and uterine natural killer (uNK) cells, and levels of progesterone and 17β-estradiol, as determined by an 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay, immunohistochemistry, and enzyme-linked immunosorbent assay, respectively. We also were measured fetal and placental growth parameters on GD 18.5. RESULTS: We found that high doses of DEX were associated with an increased abortion rate, enhancement of the immunosuppressive effect of the decidua, alterations in placental growth parameters, decreased progesterone and 17β-estradiol levels, and a reduced frequency of macrophages and uNK cells. CONCLUSION: Our data suggest that the high-dose administration of DEX during early pregnancy negatively affected pregnancy outcomes. The Korean Society for Reproductive Medicine 2016-03 2016-03-31 /pmc/articles/PMC4838577/ /pubmed/27104153 http://dx.doi.org/10.5653/cerm.2016.43.1.15 Text en Copyright © 2016. The Korean Society for Reproductive Medicine http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Namdar Ahmadabad, Hasan Kayvan Jafari, Sabah Nezafat Firizi, Maryam Abbaspour, Ali Reza Ghafoori Gharib, Fahime Ghobadi, Yusef Gholizadeh, Samira Pregnancy outcomes following the administration of high doses of dexamethasone in early pregnancy |
title | Pregnancy outcomes following the administration of high doses of dexamethasone in early pregnancy |
title_full | Pregnancy outcomes following the administration of high doses of dexamethasone in early pregnancy |
title_fullStr | Pregnancy outcomes following the administration of high doses of dexamethasone in early pregnancy |
title_full_unstemmed | Pregnancy outcomes following the administration of high doses of dexamethasone in early pregnancy |
title_short | Pregnancy outcomes following the administration of high doses of dexamethasone in early pregnancy |
title_sort | pregnancy outcomes following the administration of high doses of dexamethasone in early pregnancy |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838577/ https://www.ncbi.nlm.nih.gov/pubmed/27104153 http://dx.doi.org/10.5653/cerm.2016.43.1.15 |
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