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Bacterial Epimerization as a Route for Deoxynivalenol Detoxification: the Influence of Growth and Environmental Conditions

Deoxynivalenol (DON) is a toxic secondary metabolite produced by several Fusarium species that infest wheat and corn. Food and feed contaminated with DON pose a health risk to both humans and livestock and form a major barrier for international trade. Microbial detoxification represents an alternati...

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Autores principales: He, Jian Wei, Hassan, Yousef I., Perilla, Norma, Li, Xiu-Zhen, Boland, Greg J., Zhou, Ting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838601/
https://www.ncbi.nlm.nih.gov/pubmed/27148248
http://dx.doi.org/10.3389/fmicb.2016.00572
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author He, Jian Wei
Hassan, Yousef I.
Perilla, Norma
Li, Xiu-Zhen
Boland, Greg J.
Zhou, Ting
author_facet He, Jian Wei
Hassan, Yousef I.
Perilla, Norma
Li, Xiu-Zhen
Boland, Greg J.
Zhou, Ting
author_sort He, Jian Wei
collection PubMed
description Deoxynivalenol (DON) is a toxic secondary metabolite produced by several Fusarium species that infest wheat and corn. Food and feed contaminated with DON pose a health risk to both humans and livestock and form a major barrier for international trade. Microbial detoxification represents an alternative approach to the physical and chemical detoxification methods of DON-contaminated grains. The present study details the characterization of a novel bacterium, Devosia mutans 17-2-E-8, that is capable of transforming DON to a non-toxic stereoisomer, 3-epi-deoxynivalenol under aerobic conditions, mild temperature (25–30°C), and neutral pH. The biotransformation takes place in the presence of rich sources of organic nitrogen and carbon without the need of DON to be the sole carbon source. The process is enzymatic in nature and endures a high detoxification capacity (3 μg DON/h/10(8) cells). The above conditions collectively suggest the possibility of utilizing the isolated bacterium as a feed treatment to address DON contamination under empirical field conditions.
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spelling pubmed-48386012016-05-04 Bacterial Epimerization as a Route for Deoxynivalenol Detoxification: the Influence of Growth and Environmental Conditions He, Jian Wei Hassan, Yousef I. Perilla, Norma Li, Xiu-Zhen Boland, Greg J. Zhou, Ting Front Microbiol Microbiology Deoxynivalenol (DON) is a toxic secondary metabolite produced by several Fusarium species that infest wheat and corn. Food and feed contaminated with DON pose a health risk to both humans and livestock and form a major barrier for international trade. Microbial detoxification represents an alternative approach to the physical and chemical detoxification methods of DON-contaminated grains. The present study details the characterization of a novel bacterium, Devosia mutans 17-2-E-8, that is capable of transforming DON to a non-toxic stereoisomer, 3-epi-deoxynivalenol under aerobic conditions, mild temperature (25–30°C), and neutral pH. The biotransformation takes place in the presence of rich sources of organic nitrogen and carbon without the need of DON to be the sole carbon source. The process is enzymatic in nature and endures a high detoxification capacity (3 μg DON/h/10(8) cells). The above conditions collectively suggest the possibility of utilizing the isolated bacterium as a feed treatment to address DON contamination under empirical field conditions. Frontiers Media S.A. 2016-04-21 /pmc/articles/PMC4838601/ /pubmed/27148248 http://dx.doi.org/10.3389/fmicb.2016.00572 Text en Copyright © 2016 He, Hassan, Perilla, Li, Boland and Zhou. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
He, Jian Wei
Hassan, Yousef I.
Perilla, Norma
Li, Xiu-Zhen
Boland, Greg J.
Zhou, Ting
Bacterial Epimerization as a Route for Deoxynivalenol Detoxification: the Influence of Growth and Environmental Conditions
title Bacterial Epimerization as a Route for Deoxynivalenol Detoxification: the Influence of Growth and Environmental Conditions
title_full Bacterial Epimerization as a Route for Deoxynivalenol Detoxification: the Influence of Growth and Environmental Conditions
title_fullStr Bacterial Epimerization as a Route for Deoxynivalenol Detoxification: the Influence of Growth and Environmental Conditions
title_full_unstemmed Bacterial Epimerization as a Route for Deoxynivalenol Detoxification: the Influence of Growth and Environmental Conditions
title_short Bacterial Epimerization as a Route for Deoxynivalenol Detoxification: the Influence of Growth and Environmental Conditions
title_sort bacterial epimerization as a route for deoxynivalenol detoxification: the influence of growth and environmental conditions
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838601/
https://www.ncbi.nlm.nih.gov/pubmed/27148248
http://dx.doi.org/10.3389/fmicb.2016.00572
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