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Improved Productivity of Neutral Lipids in Chlorella sp. A2 by Minimal Nitrogen Supply
Nitrogen starvation is an efficient environmental pressure for increasing lipid accumulation in microalgae, but it could also significantly lower the biomass productivity, resulting in lower lipid productivity. In this study, green alga Chlorella sp. A2 was cultivated by using a minimal nitrogen sup...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838625/ https://www.ncbi.nlm.nih.gov/pubmed/27148237 http://dx.doi.org/10.3389/fmicb.2016.00557 |
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author | Zhu, Junying Chen, Weixian Chen, Hui Zhang, Xin He, Chenliu Rong, Junfeng Wang, Qiang |
author_facet | Zhu, Junying Chen, Weixian Chen, Hui Zhang, Xin He, Chenliu Rong, Junfeng Wang, Qiang |
author_sort | Zhu, Junying |
collection | PubMed |
description | Nitrogen starvation is an efficient environmental pressure for increasing lipid accumulation in microalgae, but it could also significantly lower the biomass productivity, resulting in lower lipid productivity. In this study, green alga Chlorella sp. A2 was cultivated by using a minimal nitrogen supply strategy under both laboratory and outdoor cultivation conditions to evaluate biomass accumulation and lipid production. Results showed that minimal nitrogen supply could promote neutral lipid accumulation of Chlorella sp. A2 without a significant negative effect on cell growth. In laboratory cultivation mode, alga cells cultured with 18 mg L(−1) d(−1) urea addition could generate 74 and 416% (w/w) more neutral lipid productivity than cells cultured with regular BG11 and nitrogen starvation media, respectively. In outdoor cultivation mode, lipid productivity of cells cultured with 18 mg L(−1) d(−1) urea addition is approximately 10 and 88% higher than the one with regular BG11 and nitrogen starvation media, respectively. Notably, the results of photosynthetic analysis clarified that minimal nitrogen supply reduced the loss of photosynthetic capacity to keep CO(2) fixation during photosynthesis for biomass production. The minimal nitrogen supply strategy for microalgae cultivation could promote neutral lipid accumulation without a significant negative effect on cell growth, resulting in a significant improvement in the lipid productivity. |
format | Online Article Text |
id | pubmed-4838625 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-48386252016-05-04 Improved Productivity of Neutral Lipids in Chlorella sp. A2 by Minimal Nitrogen Supply Zhu, Junying Chen, Weixian Chen, Hui Zhang, Xin He, Chenliu Rong, Junfeng Wang, Qiang Front Microbiol Microbiology Nitrogen starvation is an efficient environmental pressure for increasing lipid accumulation in microalgae, but it could also significantly lower the biomass productivity, resulting in lower lipid productivity. In this study, green alga Chlorella sp. A2 was cultivated by using a minimal nitrogen supply strategy under both laboratory and outdoor cultivation conditions to evaluate biomass accumulation and lipid production. Results showed that minimal nitrogen supply could promote neutral lipid accumulation of Chlorella sp. A2 without a significant negative effect on cell growth. In laboratory cultivation mode, alga cells cultured with 18 mg L(−1) d(−1) urea addition could generate 74 and 416% (w/w) more neutral lipid productivity than cells cultured with regular BG11 and nitrogen starvation media, respectively. In outdoor cultivation mode, lipid productivity of cells cultured with 18 mg L(−1) d(−1) urea addition is approximately 10 and 88% higher than the one with regular BG11 and nitrogen starvation media, respectively. Notably, the results of photosynthetic analysis clarified that minimal nitrogen supply reduced the loss of photosynthetic capacity to keep CO(2) fixation during photosynthesis for biomass production. The minimal nitrogen supply strategy for microalgae cultivation could promote neutral lipid accumulation without a significant negative effect on cell growth, resulting in a significant improvement in the lipid productivity. Frontiers Media S.A. 2016-04-21 /pmc/articles/PMC4838625/ /pubmed/27148237 http://dx.doi.org/10.3389/fmicb.2016.00557 Text en Copyright © 2016 Zhu, Chen, Chen, Zhang, He, Rong and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Zhu, Junying Chen, Weixian Chen, Hui Zhang, Xin He, Chenliu Rong, Junfeng Wang, Qiang Improved Productivity of Neutral Lipids in Chlorella sp. A2 by Minimal Nitrogen Supply |
title | Improved Productivity of Neutral Lipids in Chlorella sp. A2 by Minimal Nitrogen Supply |
title_full | Improved Productivity of Neutral Lipids in Chlorella sp. A2 by Minimal Nitrogen Supply |
title_fullStr | Improved Productivity of Neutral Lipids in Chlorella sp. A2 by Minimal Nitrogen Supply |
title_full_unstemmed | Improved Productivity of Neutral Lipids in Chlorella sp. A2 by Minimal Nitrogen Supply |
title_short | Improved Productivity of Neutral Lipids in Chlorella sp. A2 by Minimal Nitrogen Supply |
title_sort | improved productivity of neutral lipids in chlorella sp. a2 by minimal nitrogen supply |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838625/ https://www.ncbi.nlm.nih.gov/pubmed/27148237 http://dx.doi.org/10.3389/fmicb.2016.00557 |
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