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Genetic encoding of DNA nanostructures and their self-assembly in living bacteria
The field of DNA nanotechnology has harnessed the programmability of DNA base pairing to direct single-stranded DNAs (ssDNAs) to assemble into desired 3D structures. Here, we show the ability to express ssDNAs in Escherichia coli (32–205 nt), which can form structures in vivo or be purified for in v...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838831/ https://www.ncbi.nlm.nih.gov/pubmed/27091073 http://dx.doi.org/10.1038/ncomms11179 |
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author | Elbaz, Johann Yin, Peng Voigt, Christopher A. |
author_facet | Elbaz, Johann Yin, Peng Voigt, Christopher A. |
author_sort | Elbaz, Johann |
collection | PubMed |
description | The field of DNA nanotechnology has harnessed the programmability of DNA base pairing to direct single-stranded DNAs (ssDNAs) to assemble into desired 3D structures. Here, we show the ability to express ssDNAs in Escherichia coli (32–205 nt), which can form structures in vivo or be purified for in vitro assembly. Each ssDNA is encoded by a gene that is transcribed into non-coding RNA containing a 3′-hairpin (HTBS). HTBS recruits HIV reverse transcriptase, which nucleates DNA synthesis and is aided in elongation by murine leukemia reverse transcriptase. Purified ssDNA that is produced in vivo is used to assemble large 1D wires (300 nm) and 2D sheets (5.8 μm(2)) in vitro. Intracellular assembly is demonstrated using a four-ssDNA crossover nanostructure that recruits split YFP when properly assembled. Genetically encoding DNA nanostructures provides a route for their production as well as applications in living cells. |
format | Online Article Text |
id | pubmed-4838831 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48388312016-05-04 Genetic encoding of DNA nanostructures and their self-assembly in living bacteria Elbaz, Johann Yin, Peng Voigt, Christopher A. Nat Commun Article The field of DNA nanotechnology has harnessed the programmability of DNA base pairing to direct single-stranded DNAs (ssDNAs) to assemble into desired 3D structures. Here, we show the ability to express ssDNAs in Escherichia coli (32–205 nt), which can form structures in vivo or be purified for in vitro assembly. Each ssDNA is encoded by a gene that is transcribed into non-coding RNA containing a 3′-hairpin (HTBS). HTBS recruits HIV reverse transcriptase, which nucleates DNA synthesis and is aided in elongation by murine leukemia reverse transcriptase. Purified ssDNA that is produced in vivo is used to assemble large 1D wires (300 nm) and 2D sheets (5.8 μm(2)) in vitro. Intracellular assembly is demonstrated using a four-ssDNA crossover nanostructure that recruits split YFP when properly assembled. Genetically encoding DNA nanostructures provides a route for their production as well as applications in living cells. Nature Publishing Group 2016-04-19 /pmc/articles/PMC4838831/ /pubmed/27091073 http://dx.doi.org/10.1038/ncomms11179 Text en Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Elbaz, Johann Yin, Peng Voigt, Christopher A. Genetic encoding of DNA nanostructures and their self-assembly in living bacteria |
title | Genetic encoding of DNA nanostructures and their self-assembly in living bacteria |
title_full | Genetic encoding of DNA nanostructures and their self-assembly in living bacteria |
title_fullStr | Genetic encoding of DNA nanostructures and their self-assembly in living bacteria |
title_full_unstemmed | Genetic encoding of DNA nanostructures and their self-assembly in living bacteria |
title_short | Genetic encoding of DNA nanostructures and their self-assembly in living bacteria |
title_sort | genetic encoding of dna nanostructures and their self-assembly in living bacteria |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838831/ https://www.ncbi.nlm.nih.gov/pubmed/27091073 http://dx.doi.org/10.1038/ncomms11179 |
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