Cargando…

Structure, organization and evolution of ADP-ribosylation factors in rice and foxtail millet, and their expression in rice

ADP-ribosylation factors (ARFs) have been reported to function in diverse physiological and molecular activities. Recent evidences also demonstrate the involvement of ARFs in conferring tolerance to biotic and abiotic stresses in plant species. In the present study, 23 and 25 ARF proteins were ident...

Descripción completa

Detalles Bibliográficos
Autores principales: Muthamilarasan, Mehanathan, Mangu, Venkata R., Zandkarimi, Hana, Prasad, Manoj, Baisakh, Niranjan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838888/
https://www.ncbi.nlm.nih.gov/pubmed/27097755
http://dx.doi.org/10.1038/srep24008
_version_ 1782428053994995712
author Muthamilarasan, Mehanathan
Mangu, Venkata R.
Zandkarimi, Hana
Prasad, Manoj
Baisakh, Niranjan
author_facet Muthamilarasan, Mehanathan
Mangu, Venkata R.
Zandkarimi, Hana
Prasad, Manoj
Baisakh, Niranjan
author_sort Muthamilarasan, Mehanathan
collection PubMed
description ADP-ribosylation factors (ARFs) have been reported to function in diverse physiological and molecular activities. Recent evidences also demonstrate the involvement of ARFs in conferring tolerance to biotic and abiotic stresses in plant species. In the present study, 23 and 25 ARF proteins were identified in C(3) model- rice and C(4) model- foxtail millet, respectively. These proteins are classified into four classes (I–IV) based on phylogenetic analysis, with ARFs in classes I–III and ARF-like proteins (ARLs) in class IV. Sequence alignment and domain analysis revealed the presence of conserved and additional motifs, which may contribute to neo- and sub-functionalization of these proteins. Promoter analysis showed the presence of several cis-regulatory elements related to stress and hormone response, indicating their role in stress regulatory network. Expression analysis of rice ARFs and ARLs in different tissues, stresses and abscisic acid treatment highlighted temporal and spatial diversification of gene expression. Five rice cultivars screened for allelic variations in OsARF genes showed the presence of allelic polymorphisms in few gene loci. Altogether, the study provides insights on characteristics of ARF/ARL genes in rice and foxtail millet, which could be deployed for further functional analysis to extrapolate their precise roles in abiotic stress responses.
format Online
Article
Text
id pubmed-4838888
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-48388882016-04-27 Structure, organization and evolution of ADP-ribosylation factors in rice and foxtail millet, and their expression in rice Muthamilarasan, Mehanathan Mangu, Venkata R. Zandkarimi, Hana Prasad, Manoj Baisakh, Niranjan Sci Rep Article ADP-ribosylation factors (ARFs) have been reported to function in diverse physiological and molecular activities. Recent evidences also demonstrate the involvement of ARFs in conferring tolerance to biotic and abiotic stresses in plant species. In the present study, 23 and 25 ARF proteins were identified in C(3) model- rice and C(4) model- foxtail millet, respectively. These proteins are classified into four classes (I–IV) based on phylogenetic analysis, with ARFs in classes I–III and ARF-like proteins (ARLs) in class IV. Sequence alignment and domain analysis revealed the presence of conserved and additional motifs, which may contribute to neo- and sub-functionalization of these proteins. Promoter analysis showed the presence of several cis-regulatory elements related to stress and hormone response, indicating their role in stress regulatory network. Expression analysis of rice ARFs and ARLs in different tissues, stresses and abscisic acid treatment highlighted temporal and spatial diversification of gene expression. Five rice cultivars screened for allelic variations in OsARF genes showed the presence of allelic polymorphisms in few gene loci. Altogether, the study provides insights on characteristics of ARF/ARL genes in rice and foxtail millet, which could be deployed for further functional analysis to extrapolate their precise roles in abiotic stress responses. Nature Publishing Group 2016-04-21 /pmc/articles/PMC4838888/ /pubmed/27097755 http://dx.doi.org/10.1038/srep24008 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Muthamilarasan, Mehanathan
Mangu, Venkata R.
Zandkarimi, Hana
Prasad, Manoj
Baisakh, Niranjan
Structure, organization and evolution of ADP-ribosylation factors in rice and foxtail millet, and their expression in rice
title Structure, organization and evolution of ADP-ribosylation factors in rice and foxtail millet, and their expression in rice
title_full Structure, organization and evolution of ADP-ribosylation factors in rice and foxtail millet, and their expression in rice
title_fullStr Structure, organization and evolution of ADP-ribosylation factors in rice and foxtail millet, and their expression in rice
title_full_unstemmed Structure, organization and evolution of ADP-ribosylation factors in rice and foxtail millet, and their expression in rice
title_short Structure, organization and evolution of ADP-ribosylation factors in rice and foxtail millet, and their expression in rice
title_sort structure, organization and evolution of adp-ribosylation factors in rice and foxtail millet, and their expression in rice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838888/
https://www.ncbi.nlm.nih.gov/pubmed/27097755
http://dx.doi.org/10.1038/srep24008
work_keys_str_mv AT muthamilarasanmehanathan structureorganizationandevolutionofadpribosylationfactorsinriceandfoxtailmilletandtheirexpressioninrice
AT manguvenkatar structureorganizationandevolutionofadpribosylationfactorsinriceandfoxtailmilletandtheirexpressioninrice
AT zandkarimihana structureorganizationandevolutionofadpribosylationfactorsinriceandfoxtailmilletandtheirexpressioninrice
AT prasadmanoj structureorganizationandevolutionofadpribosylationfactorsinriceandfoxtailmilletandtheirexpressioninrice
AT baisakhniranjan structureorganizationandevolutionofadpribosylationfactorsinriceandfoxtailmilletandtheirexpressioninrice