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Effect of a hypercholesterolemia as a starting factor on spinal degeneration in rabbits and role of Vitamin E (α-tocopherol)

BACKGROUND: To identify the role of the hypercholesterolemia as a starting factor in discovertebral degeneration that ultimately causes lower back pain, and investigate the role of Vitamin E in this process. METHODS: The rabbits (n = 32) were divided into two broad experimental groups: A control gro...

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Autores principales: Sasani, Mehdi, Aydın, Ahmet Levent, Aytan, Nurgul, Yapicier, Ozlem, Oktenoglu, Tunc, Ozer, Nesrin Kartal, Ozer, Ali Fahir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838922/
https://www.ncbi.nlm.nih.gov/pubmed/27127701
http://dx.doi.org/10.4103/2152-7806.180092
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author Sasani, Mehdi
Aydın, Ahmet Levent
Aytan, Nurgul
Yapicier, Ozlem
Oktenoglu, Tunc
Ozer, Nesrin Kartal
Ozer, Ali Fahir
author_facet Sasani, Mehdi
Aydın, Ahmet Levent
Aytan, Nurgul
Yapicier, Ozlem
Oktenoglu, Tunc
Ozer, Nesrin Kartal
Ozer, Ali Fahir
author_sort Sasani, Mehdi
collection PubMed
description BACKGROUND: To identify the role of the hypercholesterolemia as a starting factor in discovertebral degeneration that ultimately causes lower back pain, and investigate the role of Vitamin E in this process. METHODS: The rabbits (n = 32) were divided into two broad experimental groups: A control group, and a hypercholesterolemia group, namely cholesterol, and cholesterol plus Vitamin E groups and they were fed sequentially for 4 or 8 weeks. Serum cholesterol and Vitamin E (α-tocopherol) levels were determined; vascular tissue was prepared for histopathological analyses and vertebra was decalcified for the study. RESULTS: Cholesterol diet group resulted approximately 44-fold of increase plasma cholesterol levels over the 4-week control values. Additional supplementation with Vitamin E group induced a plasma cholesterol level increase of only 37-fold as compared to the control group. In the cholesterol groups, light microscope examination revealed atherosclerotic plaque in major arteries. However, in the cholesterol plus Vitamin E treatment groups, no lipid accumulation or foam cell formation was visible in the abdominal aorta and vertebral segmental artery. In histopathological examination, we found degenerative changes in the discovertebral unit in cholesterol treated groups. CONCLUSION: Hypercholesterolemia causes fat accumulation in the disc endplate and vertebral body that causes blood supply disturbances which might be a starting factor of discovertebral degeneration. This event was not reversed by the elimination of cholesterol from the diet. Vitamin E supplementation was not effective in reducing fat accumulation in vertebral bone marrow. As a result, we conclude that degeneration of the discovertebral unit is not related to atherosclerotic changes in the major blood vessels.
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spelling pubmed-48389222016-04-28 Effect of a hypercholesterolemia as a starting factor on spinal degeneration in rabbits and role of Vitamin E (α-tocopherol) Sasani, Mehdi Aydın, Ahmet Levent Aytan, Nurgul Yapicier, Ozlem Oktenoglu, Tunc Ozer, Nesrin Kartal Ozer, Ali Fahir Surg Neurol Int Original Article BACKGROUND: To identify the role of the hypercholesterolemia as a starting factor in discovertebral degeneration that ultimately causes lower back pain, and investigate the role of Vitamin E in this process. METHODS: The rabbits (n = 32) were divided into two broad experimental groups: A control group, and a hypercholesterolemia group, namely cholesterol, and cholesterol plus Vitamin E groups and they were fed sequentially for 4 or 8 weeks. Serum cholesterol and Vitamin E (α-tocopherol) levels were determined; vascular tissue was prepared for histopathological analyses and vertebra was decalcified for the study. RESULTS: Cholesterol diet group resulted approximately 44-fold of increase plasma cholesterol levels over the 4-week control values. Additional supplementation with Vitamin E group induced a plasma cholesterol level increase of only 37-fold as compared to the control group. In the cholesterol groups, light microscope examination revealed atherosclerotic plaque in major arteries. However, in the cholesterol plus Vitamin E treatment groups, no lipid accumulation or foam cell formation was visible in the abdominal aorta and vertebral segmental artery. In histopathological examination, we found degenerative changes in the discovertebral unit in cholesterol treated groups. CONCLUSION: Hypercholesterolemia causes fat accumulation in the disc endplate and vertebral body that causes blood supply disturbances which might be a starting factor of discovertebral degeneration. This event was not reversed by the elimination of cholesterol from the diet. Vitamin E supplementation was not effective in reducing fat accumulation in vertebral bone marrow. As a result, we conclude that degeneration of the discovertebral unit is not related to atherosclerotic changes in the major blood vessels. Medknow Publications & Media Pvt Ltd 2016-04-11 /pmc/articles/PMC4838922/ /pubmed/27127701 http://dx.doi.org/10.4103/2152-7806.180092 Text en Copyright: © Surgical Neurology International http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Sasani, Mehdi
Aydın, Ahmet Levent
Aytan, Nurgul
Yapicier, Ozlem
Oktenoglu, Tunc
Ozer, Nesrin Kartal
Ozer, Ali Fahir
Effect of a hypercholesterolemia as a starting factor on spinal degeneration in rabbits and role of Vitamin E (α-tocopherol)
title Effect of a hypercholesterolemia as a starting factor on spinal degeneration in rabbits and role of Vitamin E (α-tocopherol)
title_full Effect of a hypercholesterolemia as a starting factor on spinal degeneration in rabbits and role of Vitamin E (α-tocopherol)
title_fullStr Effect of a hypercholesterolemia as a starting factor on spinal degeneration in rabbits and role of Vitamin E (α-tocopherol)
title_full_unstemmed Effect of a hypercholesterolemia as a starting factor on spinal degeneration in rabbits and role of Vitamin E (α-tocopherol)
title_short Effect of a hypercholesterolemia as a starting factor on spinal degeneration in rabbits and role of Vitamin E (α-tocopherol)
title_sort effect of a hypercholesterolemia as a starting factor on spinal degeneration in rabbits and role of vitamin e (α-tocopherol)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838922/
https://www.ncbi.nlm.nih.gov/pubmed/27127701
http://dx.doi.org/10.4103/2152-7806.180092
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