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Generation of a monoclonal antibody recognizing the CEACAM glycan structure and inhibiting adhesion using cancer tissue-originated spheroid as an antigen
Spheroids cultured directly from tumours can better reflect in vivo tumour characteristics than two-dimensional monolayer culture or three-dimensional culture of established cell lines. In this study, we generated antibodies by directly immunizing mice with primary-cultured living spheroids from hum...
Autores principales: | Sato, Yumi, Tateno, Hiroaki, Adachi, Jun, Okuyama, Hiroaki, Endo, Hiroko, Tomonaga, Takeshi, Inoue, Masahiro |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4838943/ https://www.ncbi.nlm.nih.gov/pubmed/27098764 http://dx.doi.org/10.1038/srep24823 |
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