One-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of tomato torrado virus

‘Torrado’ disease caused by tomato torrado virus (ToTV) is responsible for considerable losses in tomato production. Therefore, a one-step reverse transcription loop-mediated isothermal amplification protocol for early and fast detection of ToTV isolates has been developed. The RNA extracted from To...

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Detalles Bibliográficos
Autores principales: Budziszewska, Marta, Wieczorek, Przemysław, Obrępalska-Stęplowska, Aleksandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2016
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4839060/
https://www.ncbi.nlm.nih.gov/pubmed/26887971
http://dx.doi.org/10.1007/s00705-016-2774-2
Descripción
Sumario:‘Torrado’ disease caused by tomato torrado virus (ToTV) is responsible for considerable losses in tomato production. Therefore, a one-step reverse transcription loop-mediated isothermal amplification protocol for early and fast detection of ToTV isolates has been developed. The RNA extracted from ToTV-infected plants was tested using this protocol with a set of six primers specific for the Vp35 coat protein gene sequence. The amplified products were analyzed using amplification curves, electrophoresis, and direct staining of DNA. The sensitivity of the protocol was tenfold higher than that of conventional RT-PCR. This new protocol is inexpensive, rapid, simple, and very sensitive.

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