One-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of tomato torrado virus

‘Torrado’ disease caused by tomato torrado virus (ToTV) is responsible for considerable losses in tomato production. Therefore, a one-step reverse transcription loop-mediated isothermal amplification protocol for early and fast detection of ToTV isolates has been developed. The RNA extracted from To...

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Autores principales: Budziszewska, Marta, Wieczorek, Przemysław, Obrępalska-Stęplowska, Aleksandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2016
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4839060/
https://www.ncbi.nlm.nih.gov/pubmed/26887971
http://dx.doi.org/10.1007/s00705-016-2774-2
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author Budziszewska, Marta
Wieczorek, Przemysław
Obrępalska-Stęplowska, Aleksandra
author_facet Budziszewska, Marta
Wieczorek, Przemysław
Obrępalska-Stęplowska, Aleksandra
author_sort Budziszewska, Marta
collection PubMed
description ‘Torrado’ disease caused by tomato torrado virus (ToTV) is responsible for considerable losses in tomato production. Therefore, a one-step reverse transcription loop-mediated isothermal amplification protocol for early and fast detection of ToTV isolates has been developed. The RNA extracted from ToTV-infected plants was tested using this protocol with a set of six primers specific for the Vp35 coat protein gene sequence. The amplified products were analyzed using amplification curves, electrophoresis, and direct staining of DNA. The sensitivity of the protocol was tenfold higher than that of conventional RT-PCR. This new protocol is inexpensive, rapid, simple, and very sensitive.
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spelling pubmed-48390602016-05-11 One-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of tomato torrado virus Budziszewska, Marta Wieczorek, Przemysław Obrępalska-Stęplowska, Aleksandra Arch Virol Brief Report ‘Torrado’ disease caused by tomato torrado virus (ToTV) is responsible for considerable losses in tomato production. Therefore, a one-step reverse transcription loop-mediated isothermal amplification protocol for early and fast detection of ToTV isolates has been developed. The RNA extracted from ToTV-infected plants was tested using this protocol with a set of six primers specific for the Vp35 coat protein gene sequence. The amplified products were analyzed using amplification curves, electrophoresis, and direct staining of DNA. The sensitivity of the protocol was tenfold higher than that of conventional RT-PCR. This new protocol is inexpensive, rapid, simple, and very sensitive. Springer Vienna 2016-02-18 2016 /pmc/articles/PMC4839060/ /pubmed/26887971 http://dx.doi.org/10.1007/s00705-016-2774-2 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Brief Report
Budziszewska, Marta
Wieczorek, Przemysław
Obrępalska-Stęplowska, Aleksandra
One-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of tomato torrado virus
title One-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of tomato torrado virus
title_full One-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of tomato torrado virus
title_fullStr One-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of tomato torrado virus
title_full_unstemmed One-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of tomato torrado virus
title_short One-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of tomato torrado virus
title_sort one-step reverse transcription loop-mediated isothermal amplification (rt-lamp) for detection of tomato torrado virus
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4839060/
https://www.ncbi.nlm.nih.gov/pubmed/26887971
http://dx.doi.org/10.1007/s00705-016-2774-2
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