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Guanine-Rich Sequences Are a Dominant Feature of Exosomal microRNAs across the Mammalian Species and Cell Types

Exosome is an extracellular vesicle released from multivesicular endosomes and contains micro (mi) RNAs and functional proteins derived from the donor cells. Exosomal miRNAs act as an effector during communication with appropriate recipient cells, this can aid in the utilization of the exosomes in a...

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Autores principales: Momose, Fumiyasu, Seo, Naohiro, Akahori, Yasushi, Sawada, Shin-ichi, Harada, Naozumi, Ogura, Toru, Akiyoshi, Kazunari, Shiku, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4839687/
https://www.ncbi.nlm.nih.gov/pubmed/27101102
http://dx.doi.org/10.1371/journal.pone.0154134
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author Momose, Fumiyasu
Seo, Naohiro
Akahori, Yasushi
Sawada, Shin-ichi
Harada, Naozumi
Ogura, Toru
Akiyoshi, Kazunari
Shiku, Hiroshi
author_facet Momose, Fumiyasu
Seo, Naohiro
Akahori, Yasushi
Sawada, Shin-ichi
Harada, Naozumi
Ogura, Toru
Akiyoshi, Kazunari
Shiku, Hiroshi
author_sort Momose, Fumiyasu
collection PubMed
description Exosome is an extracellular vesicle released from multivesicular endosomes and contains micro (mi) RNAs and functional proteins derived from the donor cells. Exosomal miRNAs act as an effector during communication with appropriate recipient cells, this can aid in the utilization of the exosomes in a drug delivery system for various disorders including malignancies. Differences in the miRNA distribution pattern between exosomes and donor cells indicate the active translocation of miRNAs into the exosome cargos in a miRNA sequence-dependent manner, although the molecular mechanism is little known. In this study, we statistically analyzed the miRNA microarray data and revealed that the guanine (G)-rich sequence is a dominant feature of exosome-dominant miRNAs, across the mammalian species-specificity and the cell types. Our results provide important information regarding the potential use of exosome cargos to develop miRNA-based drugs for the treatment of human diseases.
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spelling pubmed-48396872016-04-29 Guanine-Rich Sequences Are a Dominant Feature of Exosomal microRNAs across the Mammalian Species and Cell Types Momose, Fumiyasu Seo, Naohiro Akahori, Yasushi Sawada, Shin-ichi Harada, Naozumi Ogura, Toru Akiyoshi, Kazunari Shiku, Hiroshi PLoS One Research Article Exosome is an extracellular vesicle released from multivesicular endosomes and contains micro (mi) RNAs and functional proteins derived from the donor cells. Exosomal miRNAs act as an effector during communication with appropriate recipient cells, this can aid in the utilization of the exosomes in a drug delivery system for various disorders including malignancies. Differences in the miRNA distribution pattern between exosomes and donor cells indicate the active translocation of miRNAs into the exosome cargos in a miRNA sequence-dependent manner, although the molecular mechanism is little known. In this study, we statistically analyzed the miRNA microarray data and revealed that the guanine (G)-rich sequence is a dominant feature of exosome-dominant miRNAs, across the mammalian species-specificity and the cell types. Our results provide important information regarding the potential use of exosome cargos to develop miRNA-based drugs for the treatment of human diseases. Public Library of Science 2016-04-21 /pmc/articles/PMC4839687/ /pubmed/27101102 http://dx.doi.org/10.1371/journal.pone.0154134 Text en © 2016 Momose et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Momose, Fumiyasu
Seo, Naohiro
Akahori, Yasushi
Sawada, Shin-ichi
Harada, Naozumi
Ogura, Toru
Akiyoshi, Kazunari
Shiku, Hiroshi
Guanine-Rich Sequences Are a Dominant Feature of Exosomal microRNAs across the Mammalian Species and Cell Types
title Guanine-Rich Sequences Are a Dominant Feature of Exosomal microRNAs across the Mammalian Species and Cell Types
title_full Guanine-Rich Sequences Are a Dominant Feature of Exosomal microRNAs across the Mammalian Species and Cell Types
title_fullStr Guanine-Rich Sequences Are a Dominant Feature of Exosomal microRNAs across the Mammalian Species and Cell Types
title_full_unstemmed Guanine-Rich Sequences Are a Dominant Feature of Exosomal microRNAs across the Mammalian Species and Cell Types
title_short Guanine-Rich Sequences Are a Dominant Feature of Exosomal microRNAs across the Mammalian Species and Cell Types
title_sort guanine-rich sequences are a dominant feature of exosomal micrornas across the mammalian species and cell types
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4839687/
https://www.ncbi.nlm.nih.gov/pubmed/27101102
http://dx.doi.org/10.1371/journal.pone.0154134
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