Cargando…

Metabolic Biomarker Panels of Response to Fusarium Head Blight Infection in Different Wheat Varieties

Metabolic changes in spikelets of wheat varieties FL62R1, Stettler, Muchmore and Sumai3 following Fusarium graminearum infection were explored using NMR analysis. Extensive 1D and 2D (1)H NMR measurements provided information for detailed metabolite assignment and quantification leading to possible...

Descripción completa

Detalles Bibliográficos
Autores principales: Cuperlovic-Culf, Miroslava, Wang, Lipu, Forseille, Lily, Boyle, Kerry, Merkley, Nadine, Burton, Ian, Fobert, Pierre R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4839701/
https://www.ncbi.nlm.nih.gov/pubmed/27101152
http://dx.doi.org/10.1371/journal.pone.0153642
_version_ 1782428168120958976
author Cuperlovic-Culf, Miroslava
Wang, Lipu
Forseille, Lily
Boyle, Kerry
Merkley, Nadine
Burton, Ian
Fobert, Pierre R.
author_facet Cuperlovic-Culf, Miroslava
Wang, Lipu
Forseille, Lily
Boyle, Kerry
Merkley, Nadine
Burton, Ian
Fobert, Pierre R.
author_sort Cuperlovic-Culf, Miroslava
collection PubMed
description Metabolic changes in spikelets of wheat varieties FL62R1, Stettler, Muchmore and Sumai3 following Fusarium graminearum infection were explored using NMR analysis. Extensive 1D and 2D (1)H NMR measurements provided information for detailed metabolite assignment and quantification leading to possible metabolic markers discriminating resistance level in wheat subtypes. In addition, metabolic changes that are observed in all studied varieties as well as wheat variety specific changes have been determined and discussed. A new method for metabolite quantification from NMR data that automatically aligns spectra of standards and samples prior to quantification using multivariate linear regression optimization of spectra of assigned metabolites to samples’ 1D spectra is described and utilized. Fusarium infection-induced metabolic changes in different wheat varieties are discussed in the context of metabolic network and resistance.
format Online
Article
Text
id pubmed-4839701
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-48397012016-04-29 Metabolic Biomarker Panels of Response to Fusarium Head Blight Infection in Different Wheat Varieties Cuperlovic-Culf, Miroslava Wang, Lipu Forseille, Lily Boyle, Kerry Merkley, Nadine Burton, Ian Fobert, Pierre R. PLoS One Research Article Metabolic changes in spikelets of wheat varieties FL62R1, Stettler, Muchmore and Sumai3 following Fusarium graminearum infection were explored using NMR analysis. Extensive 1D and 2D (1)H NMR measurements provided information for detailed metabolite assignment and quantification leading to possible metabolic markers discriminating resistance level in wheat subtypes. In addition, metabolic changes that are observed in all studied varieties as well as wheat variety specific changes have been determined and discussed. A new method for metabolite quantification from NMR data that automatically aligns spectra of standards and samples prior to quantification using multivariate linear regression optimization of spectra of assigned metabolites to samples’ 1D spectra is described and utilized. Fusarium infection-induced metabolic changes in different wheat varieties are discussed in the context of metabolic network and resistance. Public Library of Science 2016-04-21 /pmc/articles/PMC4839701/ /pubmed/27101152 http://dx.doi.org/10.1371/journal.pone.0153642 Text en © 2016 Cuperlovic-Culf et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Cuperlovic-Culf, Miroslava
Wang, Lipu
Forseille, Lily
Boyle, Kerry
Merkley, Nadine
Burton, Ian
Fobert, Pierre R.
Metabolic Biomarker Panels of Response to Fusarium Head Blight Infection in Different Wheat Varieties
title Metabolic Biomarker Panels of Response to Fusarium Head Blight Infection in Different Wheat Varieties
title_full Metabolic Biomarker Panels of Response to Fusarium Head Blight Infection in Different Wheat Varieties
title_fullStr Metabolic Biomarker Panels of Response to Fusarium Head Blight Infection in Different Wheat Varieties
title_full_unstemmed Metabolic Biomarker Panels of Response to Fusarium Head Blight Infection in Different Wheat Varieties
title_short Metabolic Biomarker Panels of Response to Fusarium Head Blight Infection in Different Wheat Varieties
title_sort metabolic biomarker panels of response to fusarium head blight infection in different wheat varieties
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4839701/
https://www.ncbi.nlm.nih.gov/pubmed/27101152
http://dx.doi.org/10.1371/journal.pone.0153642
work_keys_str_mv AT cuperlovicculfmiroslava metabolicbiomarkerpanelsofresponsetofusariumheadblightinfectionindifferentwheatvarieties
AT wanglipu metabolicbiomarkerpanelsofresponsetofusariumheadblightinfectionindifferentwheatvarieties
AT forseillelily metabolicbiomarkerpanelsofresponsetofusariumheadblightinfectionindifferentwheatvarieties
AT boylekerry metabolicbiomarkerpanelsofresponsetofusariumheadblightinfectionindifferentwheatvarieties
AT merkleynadine metabolicbiomarkerpanelsofresponsetofusariumheadblightinfectionindifferentwheatvarieties
AT burtonian metabolicbiomarkerpanelsofresponsetofusariumheadblightinfectionindifferentwheatvarieties
AT fobertpierrer metabolicbiomarkerpanelsofresponsetofusariumheadblightinfectionindifferentwheatvarieties