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Analysis of proteome dynamics inside the silk gland lumen of Bombyx mori

The silk gland is the only organ where silk proteins are synthesized and secreted in the silkworm, Bombyx mori. Silk proteins are stored in the lumen of the silk gland for around eight days during the fifth instar. Determining their dynamic changes is helpful for clarifying the secretion mechanism o...

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Autores principales: Dong, Zhaoming, Zhao, Ping, Zhang, Yan, Song, Qianru, Zhang, Xiaolu, Guo, Pengchao, Wang, Dandan, Xia, Qingyou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840313/
https://www.ncbi.nlm.nih.gov/pubmed/27102218
http://dx.doi.org/10.1038/srep21158
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author Dong, Zhaoming
Zhao, Ping
Zhang, Yan
Song, Qianru
Zhang, Xiaolu
Guo, Pengchao
Wang, Dandan
Xia, Qingyou
author_facet Dong, Zhaoming
Zhao, Ping
Zhang, Yan
Song, Qianru
Zhang, Xiaolu
Guo, Pengchao
Wang, Dandan
Xia, Qingyou
author_sort Dong, Zhaoming
collection PubMed
description The silk gland is the only organ where silk proteins are synthesized and secreted in the silkworm, Bombyx mori. Silk proteins are stored in the lumen of the silk gland for around eight days during the fifth instar. Determining their dynamic changes is helpful for clarifying the secretion mechanism of silk proteins. Here, we identified the proteome in the silk gland lumen using liquid chromatography–tandem mass spectrometry, and demonstrated its changes during two key stages. From day 5 of the fifth instar to day 1 of wandering, the abundances of fibroins, sericins, seroins, and proteins of unknown functions increased significantly in different compartments of the silk gland lumen. As a result, these accumulated proteins constituted the major cocoon components. In contrast, the abundances of enzymes and extracellular matrix proteins decreased in the silk gland lumen, suggesting that they were not the structural constituents of silk. Twenty-five enzymes may be involved in the regulation of hormone metabolism for proper silk gland function. In addition, the metabolism of other non-proteinous components such as chitin and pigment were also discussed in this study.
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spelling pubmed-48403132016-04-28 Analysis of proteome dynamics inside the silk gland lumen of Bombyx mori Dong, Zhaoming Zhao, Ping Zhang, Yan Song, Qianru Zhang, Xiaolu Guo, Pengchao Wang, Dandan Xia, Qingyou Sci Rep Article The silk gland is the only organ where silk proteins are synthesized and secreted in the silkworm, Bombyx mori. Silk proteins are stored in the lumen of the silk gland for around eight days during the fifth instar. Determining their dynamic changes is helpful for clarifying the secretion mechanism of silk proteins. Here, we identified the proteome in the silk gland lumen using liquid chromatography–tandem mass spectrometry, and demonstrated its changes during two key stages. From day 5 of the fifth instar to day 1 of wandering, the abundances of fibroins, sericins, seroins, and proteins of unknown functions increased significantly in different compartments of the silk gland lumen. As a result, these accumulated proteins constituted the major cocoon components. In contrast, the abundances of enzymes and extracellular matrix proteins decreased in the silk gland lumen, suggesting that they were not the structural constituents of silk. Twenty-five enzymes may be involved in the regulation of hormone metabolism for proper silk gland function. In addition, the metabolism of other non-proteinous components such as chitin and pigment were also discussed in this study. Nature Publishing Group 2016-04-22 /pmc/articles/PMC4840313/ /pubmed/27102218 http://dx.doi.org/10.1038/srep21158 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Dong, Zhaoming
Zhao, Ping
Zhang, Yan
Song, Qianru
Zhang, Xiaolu
Guo, Pengchao
Wang, Dandan
Xia, Qingyou
Analysis of proteome dynamics inside the silk gland lumen of Bombyx mori
title Analysis of proteome dynamics inside the silk gland lumen of Bombyx mori
title_full Analysis of proteome dynamics inside the silk gland lumen of Bombyx mori
title_fullStr Analysis of proteome dynamics inside the silk gland lumen of Bombyx mori
title_full_unstemmed Analysis of proteome dynamics inside the silk gland lumen of Bombyx mori
title_short Analysis of proteome dynamics inside the silk gland lumen of Bombyx mori
title_sort analysis of proteome dynamics inside the silk gland lumen of bombyx mori
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840313/
https://www.ncbi.nlm.nih.gov/pubmed/27102218
http://dx.doi.org/10.1038/srep21158
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