Cargando…
Analysis of proteome dynamics inside the silk gland lumen of Bombyx mori
The silk gland is the only organ where silk proteins are synthesized and secreted in the silkworm, Bombyx mori. Silk proteins are stored in the lumen of the silk gland for around eight days during the fifth instar. Determining their dynamic changes is helpful for clarifying the secretion mechanism o...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840313/ https://www.ncbi.nlm.nih.gov/pubmed/27102218 http://dx.doi.org/10.1038/srep21158 |
_version_ | 1782428260966072320 |
---|---|
author | Dong, Zhaoming Zhao, Ping Zhang, Yan Song, Qianru Zhang, Xiaolu Guo, Pengchao Wang, Dandan Xia, Qingyou |
author_facet | Dong, Zhaoming Zhao, Ping Zhang, Yan Song, Qianru Zhang, Xiaolu Guo, Pengchao Wang, Dandan Xia, Qingyou |
author_sort | Dong, Zhaoming |
collection | PubMed |
description | The silk gland is the only organ where silk proteins are synthesized and secreted in the silkworm, Bombyx mori. Silk proteins are stored in the lumen of the silk gland for around eight days during the fifth instar. Determining their dynamic changes is helpful for clarifying the secretion mechanism of silk proteins. Here, we identified the proteome in the silk gland lumen using liquid chromatography–tandem mass spectrometry, and demonstrated its changes during two key stages. From day 5 of the fifth instar to day 1 of wandering, the abundances of fibroins, sericins, seroins, and proteins of unknown functions increased significantly in different compartments of the silk gland lumen. As a result, these accumulated proteins constituted the major cocoon components. In contrast, the abundances of enzymes and extracellular matrix proteins decreased in the silk gland lumen, suggesting that they were not the structural constituents of silk. Twenty-five enzymes may be involved in the regulation of hormone metabolism for proper silk gland function. In addition, the metabolism of other non-proteinous components such as chitin and pigment were also discussed in this study. |
format | Online Article Text |
id | pubmed-4840313 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48403132016-04-28 Analysis of proteome dynamics inside the silk gland lumen of Bombyx mori Dong, Zhaoming Zhao, Ping Zhang, Yan Song, Qianru Zhang, Xiaolu Guo, Pengchao Wang, Dandan Xia, Qingyou Sci Rep Article The silk gland is the only organ where silk proteins are synthesized and secreted in the silkworm, Bombyx mori. Silk proteins are stored in the lumen of the silk gland for around eight days during the fifth instar. Determining their dynamic changes is helpful for clarifying the secretion mechanism of silk proteins. Here, we identified the proteome in the silk gland lumen using liquid chromatography–tandem mass spectrometry, and demonstrated its changes during two key stages. From day 5 of the fifth instar to day 1 of wandering, the abundances of fibroins, sericins, seroins, and proteins of unknown functions increased significantly in different compartments of the silk gland lumen. As a result, these accumulated proteins constituted the major cocoon components. In contrast, the abundances of enzymes and extracellular matrix proteins decreased in the silk gland lumen, suggesting that they were not the structural constituents of silk. Twenty-five enzymes may be involved in the regulation of hormone metabolism for proper silk gland function. In addition, the metabolism of other non-proteinous components such as chitin and pigment were also discussed in this study. Nature Publishing Group 2016-04-22 /pmc/articles/PMC4840313/ /pubmed/27102218 http://dx.doi.org/10.1038/srep21158 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Dong, Zhaoming Zhao, Ping Zhang, Yan Song, Qianru Zhang, Xiaolu Guo, Pengchao Wang, Dandan Xia, Qingyou Analysis of proteome dynamics inside the silk gland lumen of Bombyx mori |
title | Analysis of proteome dynamics inside the silk gland lumen of Bombyx mori |
title_full | Analysis of proteome dynamics inside the silk gland lumen of Bombyx mori |
title_fullStr | Analysis of proteome dynamics inside the silk gland lumen of Bombyx mori |
title_full_unstemmed | Analysis of proteome dynamics inside the silk gland lumen of Bombyx mori |
title_short | Analysis of proteome dynamics inside the silk gland lumen of Bombyx mori |
title_sort | analysis of proteome dynamics inside the silk gland lumen of bombyx mori |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840313/ https://www.ncbi.nlm.nih.gov/pubmed/27102218 http://dx.doi.org/10.1038/srep21158 |
work_keys_str_mv | AT dongzhaoming analysisofproteomedynamicsinsidethesilkglandlumenofbombyxmori AT zhaoping analysisofproteomedynamicsinsidethesilkglandlumenofbombyxmori AT zhangyan analysisofproteomedynamicsinsidethesilkglandlumenofbombyxmori AT songqianru analysisofproteomedynamicsinsidethesilkglandlumenofbombyxmori AT zhangxiaolu analysisofproteomedynamicsinsidethesilkglandlumenofbombyxmori AT guopengchao analysisofproteomedynamicsinsidethesilkglandlumenofbombyxmori AT wangdandan analysisofproteomedynamicsinsidethesilkglandlumenofbombyxmori AT xiaqingyou analysisofproteomedynamicsinsidethesilkglandlumenofbombyxmori |