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Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections
Berberine is an isoquinoline alkaloid widely used in the treatment of microbial infections. Recent studies have shown that berberine can enhance the inhibitory efficacy of antibiotics against clinical multi-drug resistant isolates of methicillin-resistant Staphylococcus aureus (MRSA). However, the u...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840435/ https://www.ncbi.nlm.nih.gov/pubmed/27103062 http://dx.doi.org/10.1038/srep24748 |
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author | Chu, Ming Zhang, Ming-bo Liu, Yan-chen Kang, Jia-rui Chu, Zheng-yun Yin, Kai-lin Ding, Ling-yu Ding, Ran Xiao, Rong-xin Yin, Yi-nan Liu, Xiao-yan Wang, Yue-dan |
author_facet | Chu, Ming Zhang, Ming-bo Liu, Yan-chen Kang, Jia-rui Chu, Zheng-yun Yin, Kai-lin Ding, Ling-yu Ding, Ran Xiao, Rong-xin Yin, Yi-nan Liu, Xiao-yan Wang, Yue-dan |
author_sort | Chu, Ming |
collection | PubMed |
description | Berberine is an isoquinoline alkaloid widely used in the treatment of microbial infections. Recent studies have shown that berberine can enhance the inhibitory efficacy of antibiotics against clinical multi-drug resistant isolates of methicillin-resistant Staphylococcus aureus (MRSA). However, the underlying mechanisms are poorly understood. Here, we demonstrated that sub-minimum inhibitory concentrations (MICs) of berberine exhibited no bactericidal activity against MRSA, but affected MRSA biofilm development in a dose dependent manner within the concentration ranging from 1 to 64 μg/mL. Further study indicated that berberine inhibited MRSA amyloid fibrils formation, which consist of phenol-soluble modulins (PSMs). Molecular dynamics simulation revealed that berberine could bind with the phenyl ring of Phe19 in PSMα2 through hydrophobic interaction. Collectively, berberine can inhibit MRSA biofilm formation via affecting PSMs’ aggregation into amyloid fibrils, and thereby enhance bactericidal activity of antibiotics. These findings will provide new insights into the multiple pharmacological properties of berberine in the treatment of microbial-generated amyloid involved diseases. |
format | Online Article Text |
id | pubmed-4840435 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48404352016-04-28 Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections Chu, Ming Zhang, Ming-bo Liu, Yan-chen Kang, Jia-rui Chu, Zheng-yun Yin, Kai-lin Ding, Ling-yu Ding, Ran Xiao, Rong-xin Yin, Yi-nan Liu, Xiao-yan Wang, Yue-dan Sci Rep Article Berberine is an isoquinoline alkaloid widely used in the treatment of microbial infections. Recent studies have shown that berberine can enhance the inhibitory efficacy of antibiotics against clinical multi-drug resistant isolates of methicillin-resistant Staphylococcus aureus (MRSA). However, the underlying mechanisms are poorly understood. Here, we demonstrated that sub-minimum inhibitory concentrations (MICs) of berberine exhibited no bactericidal activity against MRSA, but affected MRSA biofilm development in a dose dependent manner within the concentration ranging from 1 to 64 μg/mL. Further study indicated that berberine inhibited MRSA amyloid fibrils formation, which consist of phenol-soluble modulins (PSMs). Molecular dynamics simulation revealed that berberine could bind with the phenyl ring of Phe19 in PSMα2 through hydrophobic interaction. Collectively, berberine can inhibit MRSA biofilm formation via affecting PSMs’ aggregation into amyloid fibrils, and thereby enhance bactericidal activity of antibiotics. These findings will provide new insights into the multiple pharmacological properties of berberine in the treatment of microbial-generated amyloid involved diseases. Nature Publishing Group 2016-04-22 /pmc/articles/PMC4840435/ /pubmed/27103062 http://dx.doi.org/10.1038/srep24748 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Chu, Ming Zhang, Ming-bo Liu, Yan-chen Kang, Jia-rui Chu, Zheng-yun Yin, Kai-lin Ding, Ling-yu Ding, Ran Xiao, Rong-xin Yin, Yi-nan Liu, Xiao-yan Wang, Yue-dan Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections |
title | Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections |
title_full | Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections |
title_fullStr | Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections |
title_full_unstemmed | Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections |
title_short | Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections |
title_sort | role of berberine in the treatment of methicillin-resistant staphylococcus aureus infections |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840435/ https://www.ncbi.nlm.nih.gov/pubmed/27103062 http://dx.doi.org/10.1038/srep24748 |
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