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Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections

Berberine is an isoquinoline alkaloid widely used in the treatment of microbial infections. Recent studies have shown that berberine can enhance the inhibitory efficacy of antibiotics against clinical multi-drug resistant isolates of methicillin-resistant Staphylococcus aureus (MRSA). However, the u...

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Autores principales: Chu, Ming, Zhang, Ming-bo, Liu, Yan-chen, Kang, Jia-rui, Chu, Zheng-yun, Yin, Kai-lin, Ding, Ling-yu, Ding, Ran, Xiao, Rong-xin, Yin, Yi-nan, Liu, Xiao-yan, Wang, Yue-dan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840435/
https://www.ncbi.nlm.nih.gov/pubmed/27103062
http://dx.doi.org/10.1038/srep24748
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author Chu, Ming
Zhang, Ming-bo
Liu, Yan-chen
Kang, Jia-rui
Chu, Zheng-yun
Yin, Kai-lin
Ding, Ling-yu
Ding, Ran
Xiao, Rong-xin
Yin, Yi-nan
Liu, Xiao-yan
Wang, Yue-dan
author_facet Chu, Ming
Zhang, Ming-bo
Liu, Yan-chen
Kang, Jia-rui
Chu, Zheng-yun
Yin, Kai-lin
Ding, Ling-yu
Ding, Ran
Xiao, Rong-xin
Yin, Yi-nan
Liu, Xiao-yan
Wang, Yue-dan
author_sort Chu, Ming
collection PubMed
description Berberine is an isoquinoline alkaloid widely used in the treatment of microbial infections. Recent studies have shown that berberine can enhance the inhibitory efficacy of antibiotics against clinical multi-drug resistant isolates of methicillin-resistant Staphylococcus aureus (MRSA). However, the underlying mechanisms are poorly understood. Here, we demonstrated that sub-minimum inhibitory concentrations (MICs) of berberine exhibited no bactericidal activity against MRSA, but affected MRSA biofilm development in a dose dependent manner within the concentration ranging from 1 to 64 μg/mL. Further study indicated that berberine inhibited MRSA amyloid fibrils formation, which consist of phenol-soluble modulins (PSMs). Molecular dynamics simulation revealed that berberine could bind with the phenyl ring of Phe19 in PSMα2 through hydrophobic interaction. Collectively, berberine can inhibit MRSA biofilm formation via affecting PSMs’ aggregation into amyloid fibrils, and thereby enhance bactericidal activity of antibiotics. These findings will provide new insights into the multiple pharmacological properties of berberine in the treatment of microbial-generated amyloid involved diseases.
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spelling pubmed-48404352016-04-28 Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections Chu, Ming Zhang, Ming-bo Liu, Yan-chen Kang, Jia-rui Chu, Zheng-yun Yin, Kai-lin Ding, Ling-yu Ding, Ran Xiao, Rong-xin Yin, Yi-nan Liu, Xiao-yan Wang, Yue-dan Sci Rep Article Berberine is an isoquinoline alkaloid widely used in the treatment of microbial infections. Recent studies have shown that berberine can enhance the inhibitory efficacy of antibiotics against clinical multi-drug resistant isolates of methicillin-resistant Staphylococcus aureus (MRSA). However, the underlying mechanisms are poorly understood. Here, we demonstrated that sub-minimum inhibitory concentrations (MICs) of berberine exhibited no bactericidal activity against MRSA, but affected MRSA biofilm development in a dose dependent manner within the concentration ranging from 1 to 64 μg/mL. Further study indicated that berberine inhibited MRSA amyloid fibrils formation, which consist of phenol-soluble modulins (PSMs). Molecular dynamics simulation revealed that berberine could bind with the phenyl ring of Phe19 in PSMα2 through hydrophobic interaction. Collectively, berberine can inhibit MRSA biofilm formation via affecting PSMs’ aggregation into amyloid fibrils, and thereby enhance bactericidal activity of antibiotics. These findings will provide new insights into the multiple pharmacological properties of berberine in the treatment of microbial-generated amyloid involved diseases. Nature Publishing Group 2016-04-22 /pmc/articles/PMC4840435/ /pubmed/27103062 http://dx.doi.org/10.1038/srep24748 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Chu, Ming
Zhang, Ming-bo
Liu, Yan-chen
Kang, Jia-rui
Chu, Zheng-yun
Yin, Kai-lin
Ding, Ling-yu
Ding, Ran
Xiao, Rong-xin
Yin, Yi-nan
Liu, Xiao-yan
Wang, Yue-dan
Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections
title Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections
title_full Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections
title_fullStr Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections
title_full_unstemmed Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections
title_short Role of Berberine in the Treatment of Methicillin-Resistant Staphylococcus aureus Infections
title_sort role of berberine in the treatment of methicillin-resistant staphylococcus aureus infections
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840435/
https://www.ncbi.nlm.nih.gov/pubmed/27103062
http://dx.doi.org/10.1038/srep24748
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