Directed evolution of human scFvs in DT40 cells

Cells that constitutively diversify their immunoglobulin genes can be used for selection of novel antibodies and for refining existing affinities and specificities. Here, we report an adaptation of the chicken DT40 system wherein its capacity for somatic hypermutation is harnessed to evolve human an...

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Detalles Bibliográficos
Autores principales: Lim, Alfred W.Y., Williams, Gareth T., Rada, Cristina, Sale, Julian E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840456/
https://www.ncbi.nlm.nih.gov/pubmed/26519451
http://dx.doi.org/10.1093/protein/gzv058

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840456/
https://www.ncbi.nlm.nih.gov/pubmed/26519451
http://dx.doi.org/10.1093/protein/gzv058

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