Kinetic isotope effects for fast deuterium and proton exchange rates

By monitoring the effect of deuterium decoupling on the decay of transverse (15)N magnetization in D–(15)N spin pairs during multiple-refocusing echo sequences, we have determined fast D–D exchange rates k (D) and compared them with fast H–H exchange rates k (H) in tryptophan to determine the kineti...

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Autores principales: Canet, Estel, Mammoli, Daniele, Kadeřávek, Pavel, Pelupessy, Philippe, Bodenhausen, Geoffrey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2016
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840481/
https://www.ncbi.nlm.nih.gov/pubmed/27009684
http://dx.doi.org/10.1039/c5cp07459b
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author Canet, Estel
Mammoli, Daniele
Kadeřávek, Pavel
Pelupessy, Philippe
Bodenhausen, Geoffrey
author_facet Canet, Estel
Mammoli, Daniele
Kadeřávek, Pavel
Pelupessy, Philippe
Bodenhausen, Geoffrey
author_sort Canet, Estel
collection PubMed
description By monitoring the effect of deuterium decoupling on the decay of transverse (15)N magnetization in D–(15)N spin pairs during multiple-refocusing echo sequences, we have determined fast D–D exchange rates k (D) and compared them with fast H–H exchange rates k (H) in tryptophan to determine the kinetic isotope effect as a function of pH and temperature.
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spelling pubmed-48404812016-05-02 Kinetic isotope effects for fast deuterium and proton exchange rates Canet, Estel Mammoli, Daniele Kadeřávek, Pavel Pelupessy, Philippe Bodenhausen, Geoffrey Phys Chem Chem Phys Chemistry By monitoring the effect of deuterium decoupling on the decay of transverse (15)N magnetization in D–(15)N spin pairs during multiple-refocusing echo sequences, we have determined fast D–D exchange rates k (D) and compared them with fast H–H exchange rates k (H) in tryptophan to determine the kinetic isotope effect as a function of pH and temperature. Royal Society of Chemistry 2016-04-21 2016-03-24 /pmc/articles/PMC4840481/ /pubmed/27009684 http://dx.doi.org/10.1039/c5cp07459b Text en This journal is © The Royal Society of Chemistry 2016 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Canet, Estel
Mammoli, Daniele
Kadeřávek, Pavel
Pelupessy, Philippe
Bodenhausen, Geoffrey
Kinetic isotope effects for fast deuterium and proton exchange rates
title Kinetic isotope effects for fast deuterium and proton exchange rates
title_full Kinetic isotope effects for fast deuterium and proton exchange rates
title_fullStr Kinetic isotope effects for fast deuterium and proton exchange rates
title_full_unstemmed Kinetic isotope effects for fast deuterium and proton exchange rates
title_short Kinetic isotope effects for fast deuterium and proton exchange rates
title_sort kinetic isotope effects for fast deuterium and proton exchange rates
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840481/
https://www.ncbi.nlm.nih.gov/pubmed/27009684
http://dx.doi.org/10.1039/c5cp07459b
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