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Oxidored-nitro domain-containing protein 1 expression is associated with the progression of hepatocellular carcinoma

Hepatocarcinogenesis is a stepwise process during which multiple genes are altered. Understanding the molecular mechanisms that induce hepatocarcinogenesis may improve the screening, prevention and treatment of patients with hepatocellular carcinoma (HCC). In recent years, the oxidored-nitro domain-...

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Autores principales: LI, DENG-QING, QIU, MING, NIE, XIN-MIN, GUI, RONG, HUANG, MIN-ZHU
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840759/
https://www.ncbi.nlm.nih.gov/pubmed/27123053
http://dx.doi.org/10.3892/ol.2016.4362
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author LI, DENG-QING
QIU, MING
NIE, XIN-MIN
GUI, RONG
HUANG, MIN-ZHU
author_facet LI, DENG-QING
QIU, MING
NIE, XIN-MIN
GUI, RONG
HUANG, MIN-ZHU
author_sort LI, DENG-QING
collection PubMed
description Hepatocarcinogenesis is a stepwise process during which multiple genes are altered. Understanding the molecular mechanisms that induce hepatocarcinogenesis may improve the screening, prevention and treatment of patients with hepatocellular carcinoma (HCC). In recent years, the oxidored-nitro domain-containing protein 1 (NOR1) gene has been identified to have an important role in the development of HCC in vitro experiments. The current study aimed to examine the expression of NOR1 mRNA and protein expression in specimens of normal liver, hepatitis, cirrhosis and HCC, together representing the process of HCC development. Furthermore, the association between NOR1 expression and clinicopathological parameters of HCC patients was analyzed. Tissue microarrays containing the specimens of human normal liver, hepatitis, cirrhosis and HCC were purchased, and in situ hybridization and immunohistochemistry were used to detect the expression of NOR1 mRNA and protein expression, respectively. It was revealed that the positive rate of NOR1 protein and mRNA expression in the specimens of hepatitis and cirrhosis were not significantly different from that in the normal liver samples. However, the specimens of HCC exhibited an increased positive rate of NOR1 protein and mRNA expression in comparison with the normal liver samples. In addition, a higher positive rate of NOR1 protein expression was observed in HCC patients with a poor pathological differentiation grade and high tumor node metastasis (TNM) stage. In conclusion, the present study provides evidence, for the first time, of the increased expression of NOR1 in human HCC tissues, and its correlation with the pathological stage and TNM status. These findings indicate that NOR1 may be involved in the progression of HCC and it could be employed as a predictive biomarker in HCC development.
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spelling pubmed-48407592016-04-27 Oxidored-nitro domain-containing protein 1 expression is associated with the progression of hepatocellular carcinoma LI, DENG-QING QIU, MING NIE, XIN-MIN GUI, RONG HUANG, MIN-ZHU Oncol Lett Articles Hepatocarcinogenesis is a stepwise process during which multiple genes are altered. Understanding the molecular mechanisms that induce hepatocarcinogenesis may improve the screening, prevention and treatment of patients with hepatocellular carcinoma (HCC). In recent years, the oxidored-nitro domain-containing protein 1 (NOR1) gene has been identified to have an important role in the development of HCC in vitro experiments. The current study aimed to examine the expression of NOR1 mRNA and protein expression in specimens of normal liver, hepatitis, cirrhosis and HCC, together representing the process of HCC development. Furthermore, the association between NOR1 expression and clinicopathological parameters of HCC patients was analyzed. Tissue microarrays containing the specimens of human normal liver, hepatitis, cirrhosis and HCC were purchased, and in situ hybridization and immunohistochemistry were used to detect the expression of NOR1 mRNA and protein expression, respectively. It was revealed that the positive rate of NOR1 protein and mRNA expression in the specimens of hepatitis and cirrhosis were not significantly different from that in the normal liver samples. However, the specimens of HCC exhibited an increased positive rate of NOR1 protein and mRNA expression in comparison with the normal liver samples. In addition, a higher positive rate of NOR1 protein expression was observed in HCC patients with a poor pathological differentiation grade and high tumor node metastasis (TNM) stage. In conclusion, the present study provides evidence, for the first time, of the increased expression of NOR1 in human HCC tissues, and its correlation with the pathological stage and TNM status. These findings indicate that NOR1 may be involved in the progression of HCC and it could be employed as a predictive biomarker in HCC development. D.A. Spandidos 2016-05 2016-03-22 /pmc/articles/PMC4840759/ /pubmed/27123053 http://dx.doi.org/10.3892/ol.2016.4362 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
LI, DENG-QING
QIU, MING
NIE, XIN-MIN
GUI, RONG
HUANG, MIN-ZHU
Oxidored-nitro domain-containing protein 1 expression is associated with the progression of hepatocellular carcinoma
title Oxidored-nitro domain-containing protein 1 expression is associated with the progression of hepatocellular carcinoma
title_full Oxidored-nitro domain-containing protein 1 expression is associated with the progression of hepatocellular carcinoma
title_fullStr Oxidored-nitro domain-containing protein 1 expression is associated with the progression of hepatocellular carcinoma
title_full_unstemmed Oxidored-nitro domain-containing protein 1 expression is associated with the progression of hepatocellular carcinoma
title_short Oxidored-nitro domain-containing protein 1 expression is associated with the progression of hepatocellular carcinoma
title_sort oxidored-nitro domain-containing protein 1 expression is associated with the progression of hepatocellular carcinoma
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4840759/
https://www.ncbi.nlm.nih.gov/pubmed/27123053
http://dx.doi.org/10.3892/ol.2016.4362
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