Usefulness of a 16S rDNA real-time PCR to monitor neonatal sepsis and to assist in medical decision to discontinue antibiotics

Objective: To monitor the bacterial load in newborns with proven infections on the day of admission, 48 h and 7 days after treatment. Methods: Real-time PCR (qPCR) targeting the 16S rDNA. Results: The study recruited 17 newborns and the bacterial load was in general low (<50 CFU/mL). In three of...

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Detalles Bibliográficos
Autores principales: Stranieri, Inês, Kanunfre, Kelly Aparecida, Rodrigues, Jonatas Cristian, Yamamoto, Lidia, Nadaf, Maria Isabel Valdomir, Palmeira, Patricia, Okay, Thelma Suely
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2016
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841028/
https://www.ncbi.nlm.nih.gov/pubmed/26334172
http://dx.doi.org/10.3109/14767058.2015.1077223
Descripción
Sumario:Objective: To monitor the bacterial load in newborns with proven infections on the day of admission, 48 h and 7 days after treatment. Methods: Real-time PCR (qPCR) targeting the 16S rDNA. Results: The study recruited 17 newborns and the bacterial load was in general low (<50 CFU/mL). In three of four deaths, the bacterial load values increased, and in 11 of the 13 survivors the values decreased until the third evaluation. Conclusion: Considering the extreme sensitivity and high negative predictive value of qPCR, this test could help to monitor the treatment of neonatal sepsis and to assist in medical decision to discontinue antibiotics.

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