Usefulness of a 16S rDNA real-time PCR to monitor neonatal sepsis and to assist in medical decision to discontinue antibiotics

Objective: To monitor the bacterial load in newborns with proven infections on the day of admission, 48 h and 7 days after treatment. Methods: Real-time PCR (qPCR) targeting the 16S rDNA. Results: The study recruited 17 newborns and the bacterial load was in general low (<50 CFU/mL). In three of...

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Autores principales: Stranieri, Inês, Kanunfre, Kelly Aparecida, Rodrigues, Jonatas Cristian, Yamamoto, Lidia, Nadaf, Maria Isabel Valdomir, Palmeira, Patricia, Okay, Thelma Suely
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2016
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841028/
https://www.ncbi.nlm.nih.gov/pubmed/26334172
http://dx.doi.org/10.3109/14767058.2015.1077223
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author Stranieri, Inês
Kanunfre, Kelly Aparecida
Rodrigues, Jonatas Cristian
Yamamoto, Lidia
Nadaf, Maria Isabel Valdomir
Palmeira, Patricia
Okay, Thelma Suely
author_facet Stranieri, Inês
Kanunfre, Kelly Aparecida
Rodrigues, Jonatas Cristian
Yamamoto, Lidia
Nadaf, Maria Isabel Valdomir
Palmeira, Patricia
Okay, Thelma Suely
author_sort Stranieri, Inês
collection PubMed
description Objective: To monitor the bacterial load in newborns with proven infections on the day of admission, 48 h and 7 days after treatment. Methods: Real-time PCR (qPCR) targeting the 16S rDNA. Results: The study recruited 17 newborns and the bacterial load was in general low (<50 CFU/mL). In three of four deaths, the bacterial load values increased, and in 11 of the 13 survivors the values decreased until the third evaluation. Conclusion: Considering the extreme sensitivity and high negative predictive value of qPCR, this test could help to monitor the treatment of neonatal sepsis and to assist in medical decision to discontinue antibiotics.
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spelling pubmed-48410282016-04-28 Usefulness of a 16S rDNA real-time PCR to monitor neonatal sepsis and to assist in medical decision to discontinue antibiotics Stranieri, Inês Kanunfre, Kelly Aparecida Rodrigues, Jonatas Cristian Yamamoto, Lidia Nadaf, Maria Isabel Valdomir Palmeira, Patricia Okay, Thelma Suely J Matern Fetal Neonatal Med Short Report Objective: To monitor the bacterial load in newborns with proven infections on the day of admission, 48 h and 7 days after treatment. Methods: Real-time PCR (qPCR) targeting the 16S rDNA. Results: The study recruited 17 newborns and the bacterial load was in general low (<50 CFU/mL). In three of four deaths, the bacterial load values increased, and in 11 of the 13 survivors the values decreased until the third evaluation. Conclusion: Considering the extreme sensitivity and high negative predictive value of qPCR, this test could help to monitor the treatment of neonatal sepsis and to assist in medical decision to discontinue antibiotics. Taylor & Francis 2016-07-02 2015-08-28 /pmc/articles/PMC4841028/ /pubmed/26334172 http://dx.doi.org/10.3109/14767058.2015.1077223 Text en © 2015 The Author(s) Published by Taylor & Francis. http://creativecommons.org/Licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/Licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
spellingShingle Short Report
Stranieri, Inês
Kanunfre, Kelly Aparecida
Rodrigues, Jonatas Cristian
Yamamoto, Lidia
Nadaf, Maria Isabel Valdomir
Palmeira, Patricia
Okay, Thelma Suely
Usefulness of a 16S rDNA real-time PCR to monitor neonatal sepsis and to assist in medical decision to discontinue antibiotics
title Usefulness of a 16S rDNA real-time PCR to monitor neonatal sepsis and to assist in medical decision to discontinue antibiotics
title_full Usefulness of a 16S rDNA real-time PCR to monitor neonatal sepsis and to assist in medical decision to discontinue antibiotics
title_fullStr Usefulness of a 16S rDNA real-time PCR to monitor neonatal sepsis and to assist in medical decision to discontinue antibiotics
title_full_unstemmed Usefulness of a 16S rDNA real-time PCR to monitor neonatal sepsis and to assist in medical decision to discontinue antibiotics
title_short Usefulness of a 16S rDNA real-time PCR to monitor neonatal sepsis and to assist in medical decision to discontinue antibiotics
title_sort usefulness of a 16s rdna real-time pcr to monitor neonatal sepsis and to assist in medical decision to discontinue antibiotics
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841028/
https://www.ncbi.nlm.nih.gov/pubmed/26334172
http://dx.doi.org/10.3109/14767058.2015.1077223
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