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Enzyme-responsive polyion complex (PIC) nanoparticles for the targeted delivery of antimicrobial polymers
Here we present new enzyme-responsive polyion complex (PIC) nanoparticles prepared from antimicrobial poly(ethylene imine) and an anionic enzyme-responsive peptide targeting Pseudomonas aeruginosa's elastase. The synthetic conditions used to prepare these nanomaterials allowed us to optimise pa...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841106/ https://www.ncbi.nlm.nih.gov/pubmed/27148427 http://dx.doi.org/10.1039/c6py00146g |
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author | Insua, Ignacio Liamas, Evangelos Zhang, Zhenyu Peacock, Anna F. A. Krachler, Anne Marie Fernandez-Trillo, Francisco |
author_facet | Insua, Ignacio Liamas, Evangelos Zhang, Zhenyu Peacock, Anna F. A. Krachler, Anne Marie Fernandez-Trillo, Francisco |
author_sort | Insua, Ignacio |
collection | PubMed |
description | Here we present new enzyme-responsive polyion complex (PIC) nanoparticles prepared from antimicrobial poly(ethylene imine) and an anionic enzyme-responsive peptide targeting Pseudomonas aeruginosa's elastase. The synthetic conditions used to prepare these nanomaterials allowed us to optimise particle size and charge, and their stability under physiological conditions. We demonstrate that these enzyme responsive PIC nanoparticles are selectively degraded in the presence of P. aeruginosa elastase without being affected by other endogenous elastases. This enzyme-responsive PIC particle can exert an elastase-specific antimicrobial effect against P. aeruginosa without affecting non-pathogenic strains of these bacteria. These targeted enzyme-responsive PIC nanoparticles constitute a novel platform for the delivery of antimicrobial peptides and polymers, and can be a powerful tool in the current race against antimicrobial resistance. |
format | Online Article Text |
id | pubmed-4841106 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-48411062016-05-02 Enzyme-responsive polyion complex (PIC) nanoparticles for the targeted delivery of antimicrobial polymers Insua, Ignacio Liamas, Evangelos Zhang, Zhenyu Peacock, Anna F. A. Krachler, Anne Marie Fernandez-Trillo, Francisco Polym Chem Chemistry Here we present new enzyme-responsive polyion complex (PIC) nanoparticles prepared from antimicrobial poly(ethylene imine) and an anionic enzyme-responsive peptide targeting Pseudomonas aeruginosa's elastase. The synthetic conditions used to prepare these nanomaterials allowed us to optimise particle size and charge, and their stability under physiological conditions. We demonstrate that these enzyme responsive PIC nanoparticles are selectively degraded in the presence of P. aeruginosa elastase without being affected by other endogenous elastases. This enzyme-responsive PIC particle can exert an elastase-specific antimicrobial effect against P. aeruginosa without affecting non-pathogenic strains of these bacteria. These targeted enzyme-responsive PIC nanoparticles constitute a novel platform for the delivery of antimicrobial peptides and polymers, and can be a powerful tool in the current race against antimicrobial resistance. Royal Society of Chemistry 2016-04-21 2016-03-24 /pmc/articles/PMC4841106/ /pubmed/27148427 http://dx.doi.org/10.1039/c6py00146g Text en This journal is © The Royal Society of Chemistry 2016 http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Chemistry Insua, Ignacio Liamas, Evangelos Zhang, Zhenyu Peacock, Anna F. A. Krachler, Anne Marie Fernandez-Trillo, Francisco Enzyme-responsive polyion complex (PIC) nanoparticles for the targeted delivery of antimicrobial polymers |
title | Enzyme-responsive polyion complex (PIC) nanoparticles for the targeted delivery of antimicrobial polymers
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title_full | Enzyme-responsive polyion complex (PIC) nanoparticles for the targeted delivery of antimicrobial polymers
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title_fullStr | Enzyme-responsive polyion complex (PIC) nanoparticles for the targeted delivery of antimicrobial polymers
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title_full_unstemmed | Enzyme-responsive polyion complex (PIC) nanoparticles for the targeted delivery of antimicrobial polymers
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title_short | Enzyme-responsive polyion complex (PIC) nanoparticles for the targeted delivery of antimicrobial polymers
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title_sort | enzyme-responsive polyion complex (pic) nanoparticles for the targeted delivery of antimicrobial polymers |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841106/ https://www.ncbi.nlm.nih.gov/pubmed/27148427 http://dx.doi.org/10.1039/c6py00146g |
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