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Histopathologic evaluation of postmortem autolytic changes in bluegill (Lepomis macrohirus) and crappie (Pomoxis anularis) at varied time intervals and storage temperatures

Information is lacking on preserving fish carcasses to minimize postmortem autolysis artifacts when a necropsy cannot be performed immediately. The purpose of this study was to qualitatively identify and score histologic postmortem changes in two species of freshwater fish (bluegill—Lepomis macrochi...

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Autores principales: George, Jami, Van Wettere, Arnaud J., Michaels, Blayk B., Crain, Debbi, Lewbart, Gregory A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841231/
https://www.ncbi.nlm.nih.gov/pubmed/27114885
http://dx.doi.org/10.7717/peerj.1943
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author George, Jami
Van Wettere, Arnaud J.
Michaels, Blayk B.
Crain, Debbi
Lewbart, Gregory A.
author_facet George, Jami
Van Wettere, Arnaud J.
Michaels, Blayk B.
Crain, Debbi
Lewbart, Gregory A.
author_sort George, Jami
collection PubMed
description Information is lacking on preserving fish carcasses to minimize postmortem autolysis artifacts when a necropsy cannot be performed immediately. The purpose of this study was to qualitatively identify and score histologic postmortem changes in two species of freshwater fish (bluegill—Lepomis macrochirus; crappie—Pomoxis annularis), at varied time intervals and storage temperatures, to assess the histologic quality of collected samples. A pooled sample of 36 mix sex individuals of healthy bluegill and crappie were euthanized, stored either at room temperature, refrigerated at 4 °C, or frozen at −20 °C, and then necropsied at 0, 4, 24, and 48 h intervals. Histologic specimens were evaluated by light microscopy. Data showed that immediate harvesting of fresh samples provides the best quality and refrigeration would be the preferred method of storage if sample collection had to be delayed for up to 24 h. When sample collection must be delayed more than 24 h, the preferred method of storage to minimize autolysis artifacts is freezing if evaluation of the gastrointestinal tract is most important, or refrigeration if gill histology is most important. The gill arch, intestinal tract, followed by the liver and kidney were the most sensitive organs to autolysis.
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spelling pubmed-48412312016-04-25 Histopathologic evaluation of postmortem autolytic changes in bluegill (Lepomis macrohirus) and crappie (Pomoxis anularis) at varied time intervals and storage temperatures George, Jami Van Wettere, Arnaud J. Michaels, Blayk B. Crain, Debbi Lewbart, Gregory A. PeerJ Aquaculture, Fisheries and Fish Science Information is lacking on preserving fish carcasses to minimize postmortem autolysis artifacts when a necropsy cannot be performed immediately. The purpose of this study was to qualitatively identify and score histologic postmortem changes in two species of freshwater fish (bluegill—Lepomis macrochirus; crappie—Pomoxis annularis), at varied time intervals and storage temperatures, to assess the histologic quality of collected samples. A pooled sample of 36 mix sex individuals of healthy bluegill and crappie were euthanized, stored either at room temperature, refrigerated at 4 °C, or frozen at −20 °C, and then necropsied at 0, 4, 24, and 48 h intervals. Histologic specimens were evaluated by light microscopy. Data showed that immediate harvesting of fresh samples provides the best quality and refrigeration would be the preferred method of storage if sample collection had to be delayed for up to 24 h. When sample collection must be delayed more than 24 h, the preferred method of storage to minimize autolysis artifacts is freezing if evaluation of the gastrointestinal tract is most important, or refrigeration if gill histology is most important. The gill arch, intestinal tract, followed by the liver and kidney were the most sensitive organs to autolysis. PeerJ Inc. 2016-04-19 /pmc/articles/PMC4841231/ /pubmed/27114885 http://dx.doi.org/10.7717/peerj.1943 Text en ©2016 George et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Aquaculture, Fisheries and Fish Science
George, Jami
Van Wettere, Arnaud J.
Michaels, Blayk B.
Crain, Debbi
Lewbart, Gregory A.
Histopathologic evaluation of postmortem autolytic changes in bluegill (Lepomis macrohirus) and crappie (Pomoxis anularis) at varied time intervals and storage temperatures
title Histopathologic evaluation of postmortem autolytic changes in bluegill (Lepomis macrohirus) and crappie (Pomoxis anularis) at varied time intervals and storage temperatures
title_full Histopathologic evaluation of postmortem autolytic changes in bluegill (Lepomis macrohirus) and crappie (Pomoxis anularis) at varied time intervals and storage temperatures
title_fullStr Histopathologic evaluation of postmortem autolytic changes in bluegill (Lepomis macrohirus) and crappie (Pomoxis anularis) at varied time intervals and storage temperatures
title_full_unstemmed Histopathologic evaluation of postmortem autolytic changes in bluegill (Lepomis macrohirus) and crappie (Pomoxis anularis) at varied time intervals and storage temperatures
title_short Histopathologic evaluation of postmortem autolytic changes in bluegill (Lepomis macrohirus) and crappie (Pomoxis anularis) at varied time intervals and storage temperatures
title_sort histopathologic evaluation of postmortem autolytic changes in bluegill (lepomis macrohirus) and crappie (pomoxis anularis) at varied time intervals and storage temperatures
topic Aquaculture, Fisheries and Fish Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841231/
https://www.ncbi.nlm.nih.gov/pubmed/27114885
http://dx.doi.org/10.7717/peerj.1943
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