Proteomic Profile of Brucella abortus-Infected Bovine Chorioallantoic Membrane Explants

Brucella abortus is the etiological agent of bovine brucellosis, a zoonotic disease that causes significant economic losses worldwide. The differential proteomic profile of bovine chorioallantoic membrane (CAM) explants at early stages of infection with B. abortus (0.5, 2, 4, and 8 h) was determined...

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Autores principales: Mol, Juliana P. S., Pires, Simone F., Chapeaurouge, Alexander D., Perales, Jonas, Santos, Renato L., Andrade, Hélida M., Lage, Andrey P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841507/
https://www.ncbi.nlm.nih.gov/pubmed/27104343
http://dx.doi.org/10.1371/journal.pone.0154209
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author Mol, Juliana P. S.
Pires, Simone F.
Chapeaurouge, Alexander D.
Perales, Jonas
Santos, Renato L.
Andrade, Hélida M.
Lage, Andrey P.
author_facet Mol, Juliana P. S.
Pires, Simone F.
Chapeaurouge, Alexander D.
Perales, Jonas
Santos, Renato L.
Andrade, Hélida M.
Lage, Andrey P.
author_sort Mol, Juliana P. S.
collection PubMed
description Brucella abortus is the etiological agent of bovine brucellosis, a zoonotic disease that causes significant economic losses worldwide. The differential proteomic profile of bovine chorioallantoic membrane (CAM) explants at early stages of infection with B. abortus (0.5, 2, 4, and 8 h) was determined. Analysis of CAM explants at 0.5 and 4 h showed the highest differences between uninfected and infected CAM explants, and therefore were used for the Differential Gel Electrophoresis (DIGE). A total of 103 spots were present in only one experimental group and were selected for identification by mass spectrometry (MALDI/ToF-ToF). Proteins only identified in extracts of CAM explants infected with B. abortus were related to recognition of PAMPs by TLR, production of reactive oxygen species, intracellular trafficking, and inflammation.
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spelling pubmed-48415072016-04-29 Proteomic Profile of Brucella abortus-Infected Bovine Chorioallantoic Membrane Explants Mol, Juliana P. S. Pires, Simone F. Chapeaurouge, Alexander D. Perales, Jonas Santos, Renato L. Andrade, Hélida M. Lage, Andrey P. PLoS One Research Article Brucella abortus is the etiological agent of bovine brucellosis, a zoonotic disease that causes significant economic losses worldwide. The differential proteomic profile of bovine chorioallantoic membrane (CAM) explants at early stages of infection with B. abortus (0.5, 2, 4, and 8 h) was determined. Analysis of CAM explants at 0.5 and 4 h showed the highest differences between uninfected and infected CAM explants, and therefore were used for the Differential Gel Electrophoresis (DIGE). A total of 103 spots were present in only one experimental group and were selected for identification by mass spectrometry (MALDI/ToF-ToF). Proteins only identified in extracts of CAM explants infected with B. abortus were related to recognition of PAMPs by TLR, production of reactive oxygen species, intracellular trafficking, and inflammation. Public Library of Science 2016-04-22 /pmc/articles/PMC4841507/ /pubmed/27104343 http://dx.doi.org/10.1371/journal.pone.0154209 Text en © 2016 Mol et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Mol, Juliana P. S.
Pires, Simone F.
Chapeaurouge, Alexander D.
Perales, Jonas
Santos, Renato L.
Andrade, Hélida M.
Lage, Andrey P.
Proteomic Profile of Brucella abortus-Infected Bovine Chorioallantoic Membrane Explants
title Proteomic Profile of Brucella abortus-Infected Bovine Chorioallantoic Membrane Explants
title_full Proteomic Profile of Brucella abortus-Infected Bovine Chorioallantoic Membrane Explants
title_fullStr Proteomic Profile of Brucella abortus-Infected Bovine Chorioallantoic Membrane Explants
title_full_unstemmed Proteomic Profile of Brucella abortus-Infected Bovine Chorioallantoic Membrane Explants
title_short Proteomic Profile of Brucella abortus-Infected Bovine Chorioallantoic Membrane Explants
title_sort proteomic profile of brucella abortus-infected bovine chorioallantoic membrane explants
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841507/
https://www.ncbi.nlm.nih.gov/pubmed/27104343
http://dx.doi.org/10.1371/journal.pone.0154209
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