Distribution and Diversity of hmw1A Among Invasive Nontypeable Haemophilus influenzae Isolates in Iran

BACKGROUND: The pathogenesis of nontypeable Haemophilus influenzae (NTHi) begins with adhesion to the rhinopharyngeal mucosa. Almost 38–80% of NTHi clinical isolates produce proteins that belong to the High Molecular Weight (HMW) family of adhesins, which are believed to facilitate colonization. MET...

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Autores principales: Shahini Shams Abadi, Milad, Siadat, Seyed Davar, Vaziri, Farzam, Davari, Mehdi, Fateh, Abolfazl, Pourazar, Shahin, Abdolrahimi, Farid, Ghazanfari, Morteza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Avicenna Research Institute 2016
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842248/
https://www.ncbi.nlm.nih.gov/pubmed/27141269
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author Shahini Shams Abadi, Milad
Siadat, Seyed Davar
Vaziri, Farzam
Davari, Mehdi
Fateh, Abolfazl
Pourazar, Shahin
Abdolrahimi, Farid
Ghazanfari, Morteza
author_facet Shahini Shams Abadi, Milad
Siadat, Seyed Davar
Vaziri, Farzam
Davari, Mehdi
Fateh, Abolfazl
Pourazar, Shahin
Abdolrahimi, Farid
Ghazanfari, Morteza
author_sort Shahini Shams Abadi, Milad
collection PubMed
description BACKGROUND: The pathogenesis of nontypeable Haemophilus influenzae (NTHi) begins with adhesion to the rhinopharyngeal mucosa. Almost 38–80% of NTHi clinical isolates produce proteins that belong to the High Molecular Weight (HMW) family of adhesins, which are believed to facilitate colonization. METHODS: In the present study, the prevalence of hmwA, which encodes the HMW adhesin, was determined for a collection of 32 NTHi isolates. Restriction Fragment Length Polymorphism (RFLP) was performed to advance our understanding of hmwA binding sequence diversity. RESULTS: The results demonstrated that hmwA was detected in 61% of NTHi isolates. According to RFLP, isolates were divided into three groups. CONCLUSION: Based on these observations, it is hypothesized that some strains of nontypeable Haemophilus influenzae infect some specific areas more than other parts.
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spelling pubmed-48422482016-05-02 Distribution and Diversity of hmw1A Among Invasive Nontypeable Haemophilus influenzae Isolates in Iran Shahini Shams Abadi, Milad Siadat, Seyed Davar Vaziri, Farzam Davari, Mehdi Fateh, Abolfazl Pourazar, Shahin Abdolrahimi, Farid Ghazanfari, Morteza Avicenna J Med Biotechnol Short Communication BACKGROUND: The pathogenesis of nontypeable Haemophilus influenzae (NTHi) begins with adhesion to the rhinopharyngeal mucosa. Almost 38–80% of NTHi clinical isolates produce proteins that belong to the High Molecular Weight (HMW) family of adhesins, which are believed to facilitate colonization. METHODS: In the present study, the prevalence of hmwA, which encodes the HMW adhesin, was determined for a collection of 32 NTHi isolates. Restriction Fragment Length Polymorphism (RFLP) was performed to advance our understanding of hmwA binding sequence diversity. RESULTS: The results demonstrated that hmwA was detected in 61% of NTHi isolates. According to RFLP, isolates were divided into three groups. CONCLUSION: Based on these observations, it is hypothesized that some strains of nontypeable Haemophilus influenzae infect some specific areas more than other parts. Avicenna Research Institute 2016 /pmc/articles/PMC4842248/ /pubmed/27141269 Text en Copyright© 2016 Avicenna Research Institute This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Short Communication
Shahini Shams Abadi, Milad
Siadat, Seyed Davar
Vaziri, Farzam
Davari, Mehdi
Fateh, Abolfazl
Pourazar, Shahin
Abdolrahimi, Farid
Ghazanfari, Morteza
Distribution and Diversity of hmw1A Among Invasive Nontypeable Haemophilus influenzae Isolates in Iran
title Distribution and Diversity of hmw1A Among Invasive Nontypeable Haemophilus influenzae Isolates in Iran
title_full Distribution and Diversity of hmw1A Among Invasive Nontypeable Haemophilus influenzae Isolates in Iran
title_fullStr Distribution and Diversity of hmw1A Among Invasive Nontypeable Haemophilus influenzae Isolates in Iran
title_full_unstemmed Distribution and Diversity of hmw1A Among Invasive Nontypeable Haemophilus influenzae Isolates in Iran
title_short Distribution and Diversity of hmw1A Among Invasive Nontypeable Haemophilus influenzae Isolates in Iran
title_sort distribution and diversity of hmw1a among invasive nontypeable haemophilus influenzae isolates in iran
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842248/
https://www.ncbi.nlm.nih.gov/pubmed/27141269
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