Cargando…

AB118. Effect of MOTILIPERM in cisplatin administered SD rats

OBJECTIVE: Cisplatin causes male infertility but the exact mechanism has not been clarified, yet. MOTILIPERM has been implicated in alleviation of infertility in Sprague-Dawley rats caused by cisplatin. We evaluated recovery effect of MOTILIPERM on cisplatin (CIS)-induced testicular toxicity in Spra...

Descripción completa

Detalles Bibliográficos
Autores principales: Soni, Kiran Kumar, Zhang, Li Tao, Choi, Bo Ram, Shin, Yu Seob, Park, Jong Kwan, Lee, Sung Won, Kim, Chul Young, Cui, Wan-Shou, Chae, Han Jung, Kim, Hye Kyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842546/
http://dx.doi.org/10.21037/tau.2016.s118
_version_ 1782428539768799232
author Soni, Kiran Kumar
Zhang, Li Tao
Choi, Bo Ram
Shin, Yu Seob
Park, Jong Kwan
Lee, Sung Won
Kim, Chul Young
Cui, Wan-Shou
Chae, Han Jung
Kim, Hye Kyung
author_facet Soni, Kiran Kumar
Zhang, Li Tao
Choi, Bo Ram
Shin, Yu Seob
Park, Jong Kwan
Lee, Sung Won
Kim, Chul Young
Cui, Wan-Shou
Chae, Han Jung
Kim, Hye Kyung
author_sort Soni, Kiran Kumar
collection PubMed
description OBJECTIVE: Cisplatin causes male infertility but the exact mechanism has not been clarified, yet. MOTILIPERM has been implicated in alleviation of infertility in Sprague-Dawley rats caused by cisplatin. We evaluated recovery effect of MOTILIPERM on cisplatin (CIS)-induced testicular toxicity in Sprague-Dawley rats. METHODS: Five groups were included. The groups are control (CTR), CTR + MOTILIPERM 200 mg/kg/day per oral, CIS 10 mg/kg i.v., CIS 10 mg/kg + MOTILIPERM 100 mg/kg/day, CIS 10 mg/kg + MOTILIPERM 200 mg/kg/day. CIS 10 mg/kg i.v. single dose was given before 100 or 200 mg/kg MOTILIPERM per oral daily for 28 days. Body and genital organs weight, epididymis sperm count, sperm motility, sperm apoptosis, testosterone level, MDA of testis tissue, spermatogenic cell density, and Johnsen’s score were evaluated. Steroidogenic acute regulatory (StAR) protein, and Glucose-regulated protein-78 (GRP-78), phosphorylated inositol-requiring transmembrane kinase/endoribonuclease 1 (ire1) and phosphorylated c-jun-N-terminal kinase (p-JNK) were quantitated by western blot to show its signaling pathway. RESULTS: The body weight was decreased significantly in CIS 10 mg/kg, CIS 10 mg/kg + MOTILIPERM 100 mg/kg/day, CIS 10 mg/kg + MOTILIPERM 200 mg/kg/day compared with CTR (P<0.001) however, it was increased in CIS 10 mg/kg + MOTILIPERM 100 mg/kg/day, CIS 10 mg/kg + MOTILIPERM 200 mg/kg/day compared with CIS 10 mg/kg. The decreased weight of epididymis and prostate were increased significantly in CIS 10 mg/kg + MOTILIPERM 100 mg/kg/day compared with CIS 10 mg/kg. Sperm count, sperm motility, sperm apoptosis, MDA of testis tissue, spermatogenic cell density, Johnsen’s score, and total testosterone were also significantly improved by MOTILIPERM treatment. The levels of decreased StAR protein were significantly improved by MOTILIPERM administration, increased GRP-78 protein p-IRE1and p-JNK was also significantly decreased with MOTILIPREM treatment. CONCLUSIONS: The MOTILIPERM could be an effective medicine to reduce the toxic effect caused ER stress by CIS in the testis.
format Online
Article
Text
id pubmed-4842546
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher AME Publishing Company
record_format MEDLINE/PubMed
spelling pubmed-48425462016-05-09 AB118. Effect of MOTILIPERM in cisplatin administered SD rats Soni, Kiran Kumar Zhang, Li Tao Choi, Bo Ram Shin, Yu Seob Park, Jong Kwan Lee, Sung Won Kim, Chul Young Cui, Wan-Shou Chae, Han Jung Kim, Hye Kyung Transl Androl Urol Poster Presentation OBJECTIVE: Cisplatin causes male infertility but the exact mechanism has not been clarified, yet. MOTILIPERM has been implicated in alleviation of infertility in Sprague-Dawley rats caused by cisplatin. We evaluated recovery effect of MOTILIPERM on cisplatin (CIS)-induced testicular toxicity in Sprague-Dawley rats. METHODS: Five groups were included. The groups are control (CTR), CTR + MOTILIPERM 200 mg/kg/day per oral, CIS 10 mg/kg i.v., CIS 10 mg/kg + MOTILIPERM 100 mg/kg/day, CIS 10 mg/kg + MOTILIPERM 200 mg/kg/day. CIS 10 mg/kg i.v. single dose was given before 100 or 200 mg/kg MOTILIPERM per oral daily for 28 days. Body and genital organs weight, epididymis sperm count, sperm motility, sperm apoptosis, testosterone level, MDA of testis tissue, spermatogenic cell density, and Johnsen’s score were evaluated. Steroidogenic acute regulatory (StAR) protein, and Glucose-regulated protein-78 (GRP-78), phosphorylated inositol-requiring transmembrane kinase/endoribonuclease 1 (ire1) and phosphorylated c-jun-N-terminal kinase (p-JNK) were quantitated by western blot to show its signaling pathway. RESULTS: The body weight was decreased significantly in CIS 10 mg/kg, CIS 10 mg/kg + MOTILIPERM 100 mg/kg/day, CIS 10 mg/kg + MOTILIPERM 200 mg/kg/day compared with CTR (P<0.001) however, it was increased in CIS 10 mg/kg + MOTILIPERM 100 mg/kg/day, CIS 10 mg/kg + MOTILIPERM 200 mg/kg/day compared with CIS 10 mg/kg. The decreased weight of epididymis and prostate were increased significantly in CIS 10 mg/kg + MOTILIPERM 100 mg/kg/day compared with CIS 10 mg/kg. Sperm count, sperm motility, sperm apoptosis, MDA of testis tissue, spermatogenic cell density, Johnsen’s score, and total testosterone were also significantly improved by MOTILIPERM treatment. The levels of decreased StAR protein were significantly improved by MOTILIPERM administration, increased GRP-78 protein p-IRE1and p-JNK was also significantly decreased with MOTILIPREM treatment. CONCLUSIONS: The MOTILIPERM could be an effective medicine to reduce the toxic effect caused ER stress by CIS in the testis. AME Publishing Company 2016-04 /pmc/articles/PMC4842546/ http://dx.doi.org/10.21037/tau.2016.s118 Text en 2016 Translational Andrology and Urology. All rights reserved.
spellingShingle Poster Presentation
Soni, Kiran Kumar
Zhang, Li Tao
Choi, Bo Ram
Shin, Yu Seob
Park, Jong Kwan
Lee, Sung Won
Kim, Chul Young
Cui, Wan-Shou
Chae, Han Jung
Kim, Hye Kyung
AB118. Effect of MOTILIPERM in cisplatin administered SD rats
title AB118. Effect of MOTILIPERM in cisplatin administered SD rats
title_full AB118. Effect of MOTILIPERM in cisplatin administered SD rats
title_fullStr AB118. Effect of MOTILIPERM in cisplatin administered SD rats
title_full_unstemmed AB118. Effect of MOTILIPERM in cisplatin administered SD rats
title_short AB118. Effect of MOTILIPERM in cisplatin administered SD rats
title_sort ab118. effect of motiliperm in cisplatin administered sd rats
topic Poster Presentation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842546/
http://dx.doi.org/10.21037/tau.2016.s118
work_keys_str_mv AT sonikirankumar ab118effectofmotilipermincisplatinadministeredsdrats
AT zhanglitao ab118effectofmotilipermincisplatinadministeredsdrats
AT choiboram ab118effectofmotilipermincisplatinadministeredsdrats
AT shinyuseob ab118effectofmotilipermincisplatinadministeredsdrats
AT parkjongkwan ab118effectofmotilipermincisplatinadministeredsdrats
AT leesungwon ab118effectofmotilipermincisplatinadministeredsdrats
AT kimchulyoung ab118effectofmotilipermincisplatinadministeredsdrats
AT cuiwanshou ab118effectofmotilipermincisplatinadministeredsdrats
AT chaehanjung ab118effectofmotilipermincisplatinadministeredsdrats
AT kimhyekyung ab118effectofmotilipermincisplatinadministeredsdrats