AB128. Inhibiting cell migration and cell invasion by silencing the transcription factor ETS-1 in human bladder cancer

OBJECTIVE: ETS-1, one of the members of the ETS gene family, is an important transcription factor that plays key role in the regulation of physiological processes in normal cells and in tumors. In this study, we aimed to investigate the relationship between the transcription factor ETS-1 and malignant phenotypes of bladder cancer. METHODS: The expression pattern of ETS-1 was determined in bladder cancer tissues using qRT-PCR. Vectors expressing ETS-1 shRNA and ETS-1 protein were constructed in vitro and transfected into the human bladder cancer T24 and 5637 cells. RESULTS: ETS-1 was up-regulated in human bladder cancer tissue compared to paired normal bladder tissue. Our results showed that the transcription factor ETS-1 could promote cell migration and cell invasion in human bladder cancer, without affecting cell proliferation and immortalization. CONCLUSIONS: ETS-1 plays oncogenic roles through inducing cell migration and invasion in human bladder cancer, and it can be used as a therapeutic target for treating human bladder cancer.