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AB067. High novel oncogene with kinase-domain (NOK) gene expression is associated with the progression of renal cell carcinoma
OBJECTIVE: To study the expression levels of the NOK (novel oncogene with kinase-domain) gene in renal cell carcinoma (RCC) and its association with the progression of this cancer. METHODS: In this study, immunohistochemistry (IHC) and Western blot analyses were applied to investigate the NOK expres...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AME Publishing Company
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842618/ http://dx.doi.org/10.21037/tau.2016.s067 |
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author | Cao, Qingfei Tong, Ming Li, Zizhi Huang, Weichao Jin, Yanyang |
author_facet | Cao, Qingfei Tong, Ming Li, Zizhi Huang, Weichao Jin, Yanyang |
author_sort | Cao, Qingfei |
collection | PubMed |
description | OBJECTIVE: To study the expression levels of the NOK (novel oncogene with kinase-domain) gene in renal cell carcinoma (RCC) and its association with the progression of this cancer. METHODS: In this study, immunohistochemistry (IHC) and Western blot analyses were applied to investigate the NOK expression level in RCC and adjacent normal renal tissue samples. MTT, colony formation, and migration assays were also utilized to evaluate the role of NOK in RCC cell lines. RESULTS: Knocked-down expression of NOK in an RCC cell line (786-O) suppressed cellular proliferation and migration by restraining the activation of AKT and ERK. We found that the expression level of NOK was significantly higher in RCC tissues than in their adjacent tissues, and more importantly, overexpression of NOK was evidently correlated with the tumor TNM stage and Fuhrman grade (P<0.001). A high level of NOK was also associated with poor overall survival (P<0.05) and disease-free survival (P<0.05) by Kaplan-Meier analysis. CONCLUSIONS: NOK expression increased in RCC and was significantly correlated with TNM stage, Fuhrman grade, poor overall survival, poor disease-free survival, metastasis, and proliferation in RCC cells by regulating the activation of AKT and ERK, suggesting that NOK may play important roles as a positive regulator to RCC. |
format | Online Article Text |
id | pubmed-4842618 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | AME Publishing Company |
record_format | MEDLINE/PubMed |
spelling | pubmed-48426182016-05-09 AB067. High novel oncogene with kinase-domain (NOK) gene expression is associated with the progression of renal cell carcinoma Cao, Qingfei Tong, Ming Li, Zizhi Huang, Weichao Jin, Yanyang Transl Androl Urol Poster Presentation OBJECTIVE: To study the expression levels of the NOK (novel oncogene with kinase-domain) gene in renal cell carcinoma (RCC) and its association with the progression of this cancer. METHODS: In this study, immunohistochemistry (IHC) and Western blot analyses were applied to investigate the NOK expression level in RCC and adjacent normal renal tissue samples. MTT, colony formation, and migration assays were also utilized to evaluate the role of NOK in RCC cell lines. RESULTS: Knocked-down expression of NOK in an RCC cell line (786-O) suppressed cellular proliferation and migration by restraining the activation of AKT and ERK. We found that the expression level of NOK was significantly higher in RCC tissues than in their adjacent tissues, and more importantly, overexpression of NOK was evidently correlated with the tumor TNM stage and Fuhrman grade (P<0.001). A high level of NOK was also associated with poor overall survival (P<0.05) and disease-free survival (P<0.05) by Kaplan-Meier analysis. CONCLUSIONS: NOK expression increased in RCC and was significantly correlated with TNM stage, Fuhrman grade, poor overall survival, poor disease-free survival, metastasis, and proliferation in RCC cells by regulating the activation of AKT and ERK, suggesting that NOK may play important roles as a positive regulator to RCC. AME Publishing Company 2016-04 /pmc/articles/PMC4842618/ http://dx.doi.org/10.21037/tau.2016.s067 Text en 2016 Translational Andrology and Urology. All rights reserved. |
spellingShingle | Poster Presentation Cao, Qingfei Tong, Ming Li, Zizhi Huang, Weichao Jin, Yanyang AB067. High novel oncogene with kinase-domain (NOK) gene expression is associated with the progression of renal cell carcinoma |
title | AB067. High novel oncogene with kinase-domain (NOK) gene expression is associated with the progression of renal cell carcinoma |
title_full | AB067. High novel oncogene with kinase-domain (NOK) gene expression is associated with the progression of renal cell carcinoma |
title_fullStr | AB067. High novel oncogene with kinase-domain (NOK) gene expression is associated with the progression of renal cell carcinoma |
title_full_unstemmed | AB067. High novel oncogene with kinase-domain (NOK) gene expression is associated with the progression of renal cell carcinoma |
title_short | AB067. High novel oncogene with kinase-domain (NOK) gene expression is associated with the progression of renal cell carcinoma |
title_sort | ab067. high novel oncogene with kinase-domain (nok) gene expression is associated with the progression of renal cell carcinoma |
topic | Poster Presentation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842618/ http://dx.doi.org/10.21037/tau.2016.s067 |
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