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Genetic polymorphisms of cell adhesion molecules in Behcet’s disease in a Chinese Han population

Cell adhesion molecules (CAMs) are involved in various immune-mediated diseases. This study was conducted to investigate the association of single nucleotide polymorphisms (SNPs) of CAMs with Behçet’s disease (BD) in a Chinese Han population. A two-stage association study was carried out in 1149 BD...

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Autores principales: Zheng, Minming, Zhang, Lijun, Yu, Hongsong, Hu, Jiayue, Cao, Qingfeng, Huang, Guo, Huang, Yang, Yuan, Gangxiang, Kijlstra, Aize, Yang, Peizeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842956/
https://www.ncbi.nlm.nih.gov/pubmed/27108704
http://dx.doi.org/10.1038/srep24974
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author Zheng, Minming
Zhang, Lijun
Yu, Hongsong
Hu, Jiayue
Cao, Qingfeng
Huang, Guo
Huang, Yang
Yuan, Gangxiang
Kijlstra, Aize
Yang, Peizeng
author_facet Zheng, Minming
Zhang, Lijun
Yu, Hongsong
Hu, Jiayue
Cao, Qingfeng
Huang, Guo
Huang, Yang
Yuan, Gangxiang
Kijlstra, Aize
Yang, Peizeng
author_sort Zheng, Minming
collection PubMed
description Cell adhesion molecules (CAMs) are involved in various immune-mediated diseases. This study was conducted to investigate the association of single nucleotide polymorphisms (SNPs) of CAMs with Behçet’s disease (BD) in a Chinese Han population. A two-stage association study was carried out in 1149 BD patients and 2107 normal controls. Genotyping of 43 SNPs was performed using MassARRAY System (Sequenom), polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and TaqMan SNP assays. The expression of CD6 and CD11c was examined by real-time PCR and cytokine production was measured by ELISA. A significantly higher frequency of the CT genotype, and a lower frequency of the CC genotype and C allele of CD6 rs11230563 were observed in BD as compared with controls. Analysis of CD11c rs2929 showed that patients with BD had a significantly higher frequency of the GG genotype and G allele, and a lower frequency of the AG genotype as compared with controls. Functional experiments showed an increased CD11c expression and increased production of TNF-α and IL-1beta by LPS stimulated PBMCs in GG carriers of CD11c rs2929 compared to AA/AG carriers. Our study provides evidence that CD6 and CD11c are involved in the susceptibility to BD in a Chinese Han population.
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spelling pubmed-48429562016-04-29 Genetic polymorphisms of cell adhesion molecules in Behcet’s disease in a Chinese Han population Zheng, Minming Zhang, Lijun Yu, Hongsong Hu, Jiayue Cao, Qingfeng Huang, Guo Huang, Yang Yuan, Gangxiang Kijlstra, Aize Yang, Peizeng Sci Rep Article Cell adhesion molecules (CAMs) are involved in various immune-mediated diseases. This study was conducted to investigate the association of single nucleotide polymorphisms (SNPs) of CAMs with Behçet’s disease (BD) in a Chinese Han population. A two-stage association study was carried out in 1149 BD patients and 2107 normal controls. Genotyping of 43 SNPs was performed using MassARRAY System (Sequenom), polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and TaqMan SNP assays. The expression of CD6 and CD11c was examined by real-time PCR and cytokine production was measured by ELISA. A significantly higher frequency of the CT genotype, and a lower frequency of the CC genotype and C allele of CD6 rs11230563 were observed in BD as compared with controls. Analysis of CD11c rs2929 showed that patients with BD had a significantly higher frequency of the GG genotype and G allele, and a lower frequency of the AG genotype as compared with controls. Functional experiments showed an increased CD11c expression and increased production of TNF-α and IL-1beta by LPS stimulated PBMCs in GG carriers of CD11c rs2929 compared to AA/AG carriers. Our study provides evidence that CD6 and CD11c are involved in the susceptibility to BD in a Chinese Han population. Nature Publishing Group 2016-04-25 /pmc/articles/PMC4842956/ /pubmed/27108704 http://dx.doi.org/10.1038/srep24974 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zheng, Minming
Zhang, Lijun
Yu, Hongsong
Hu, Jiayue
Cao, Qingfeng
Huang, Guo
Huang, Yang
Yuan, Gangxiang
Kijlstra, Aize
Yang, Peizeng
Genetic polymorphisms of cell adhesion molecules in Behcet’s disease in a Chinese Han population
title Genetic polymorphisms of cell adhesion molecules in Behcet’s disease in a Chinese Han population
title_full Genetic polymorphisms of cell adhesion molecules in Behcet’s disease in a Chinese Han population
title_fullStr Genetic polymorphisms of cell adhesion molecules in Behcet’s disease in a Chinese Han population
title_full_unstemmed Genetic polymorphisms of cell adhesion molecules in Behcet’s disease in a Chinese Han population
title_short Genetic polymorphisms of cell adhesion molecules in Behcet’s disease in a Chinese Han population
title_sort genetic polymorphisms of cell adhesion molecules in behcet’s disease in a chinese han population
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4842956/
https://www.ncbi.nlm.nih.gov/pubmed/27108704
http://dx.doi.org/10.1038/srep24974
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