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Gut Microbiome Developmental Patterns in Early Life of Preterm Infants: Impacts of Feeding and Gender

Gut microbiota plays a key role in multiple aspects of human health and disease, particularly in early life. Distortions of the gut microbiota have been found to correlate with fatal diseases in preterm infants, however, developmental patterns of gut microbiome and factors affecting the colonization...

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Autores principales: Cong, Xiaomei, Xu, Wanli, Janton, Susan, Henderson, Wendy A., Matson, Adam, McGrath, Jacqueline M., Maas, Kendra, Graf, Joerg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4844123/
https://www.ncbi.nlm.nih.gov/pubmed/27111847
http://dx.doi.org/10.1371/journal.pone.0152751
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author Cong, Xiaomei
Xu, Wanli
Janton, Susan
Henderson, Wendy A.
Matson, Adam
McGrath, Jacqueline M.
Maas, Kendra
Graf, Joerg
author_facet Cong, Xiaomei
Xu, Wanli
Janton, Susan
Henderson, Wendy A.
Matson, Adam
McGrath, Jacqueline M.
Maas, Kendra
Graf, Joerg
author_sort Cong, Xiaomei
collection PubMed
description Gut microbiota plays a key role in multiple aspects of human health and disease, particularly in early life. Distortions of the gut microbiota have been found to correlate with fatal diseases in preterm infants, however, developmental patterns of gut microbiome and factors affecting the colonization progress in preterm infants remain unclear. The purpose of this prospective longitudinal study was to explore day-to-day gut microbiome patterns in preterm infants during their first 30 days of life in the neonatal intensive care unit (NICU) and investigate potential factors related to the development of the infant gut microbiome. A total of 378 stool samples were collected daily from 29 stable/healthy preterm infants. DNA extracted from stool was used to sequence the V4 region of the 16S rRNA gene region for community analysis. Operational taxonomic units (OTUs) and α-diversity of the community were determined using QIIME software. Proteobacteria was the most abundant phylum, accounting for 54.3% of the total reads. Result showed shift patterns of increasing Clostridium and Bacteroides, and decreasing Staphylococcus and Haemophilus over time during early life. Alpha-diversity significantly increased daily in preterm infants after birth and linear mixed-effects models showed that postnatal days, feeding types and gender were associated with the α-diversity, p< 0.05–0.01. Male infants were found to begin with a low α-diversity, whereas females tended to have a higher diversity shortly after birth. Female infants were more likely to have higher abundance of Clostridiates, and lower abundance of Enterobacteriales than males during early life. Infants fed mother’s own breastmilk (MBM) had a higher diversity of gut microbiome and significantly higher abundance in Clostridiales and Lactobacillales than infants fed non-MBM. Permanova also showed that bacterial compositions were different between males and females and between MBM and non-MBM feeding types. In conclusion, infant postnatal age, gender and feeding type significantly contribute to the dynamic development of the gut microbiome in preterm infants.
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spelling pubmed-48441232016-05-05 Gut Microbiome Developmental Patterns in Early Life of Preterm Infants: Impacts of Feeding and Gender Cong, Xiaomei Xu, Wanli Janton, Susan Henderson, Wendy A. Matson, Adam McGrath, Jacqueline M. Maas, Kendra Graf, Joerg PLoS One Research Article Gut microbiota plays a key role in multiple aspects of human health and disease, particularly in early life. Distortions of the gut microbiota have been found to correlate with fatal diseases in preterm infants, however, developmental patterns of gut microbiome and factors affecting the colonization progress in preterm infants remain unclear. The purpose of this prospective longitudinal study was to explore day-to-day gut microbiome patterns in preterm infants during their first 30 days of life in the neonatal intensive care unit (NICU) and investigate potential factors related to the development of the infant gut microbiome. A total of 378 stool samples were collected daily from 29 stable/healthy preterm infants. DNA extracted from stool was used to sequence the V4 region of the 16S rRNA gene region for community analysis. Operational taxonomic units (OTUs) and α-diversity of the community were determined using QIIME software. Proteobacteria was the most abundant phylum, accounting for 54.3% of the total reads. Result showed shift patterns of increasing Clostridium and Bacteroides, and decreasing Staphylococcus and Haemophilus over time during early life. Alpha-diversity significantly increased daily in preterm infants after birth and linear mixed-effects models showed that postnatal days, feeding types and gender were associated with the α-diversity, p< 0.05–0.01. Male infants were found to begin with a low α-diversity, whereas females tended to have a higher diversity shortly after birth. Female infants were more likely to have higher abundance of Clostridiates, and lower abundance of Enterobacteriales than males during early life. Infants fed mother’s own breastmilk (MBM) had a higher diversity of gut microbiome and significantly higher abundance in Clostridiales and Lactobacillales than infants fed non-MBM. Permanova also showed that bacterial compositions were different between males and females and between MBM and non-MBM feeding types. In conclusion, infant postnatal age, gender and feeding type significantly contribute to the dynamic development of the gut microbiome in preterm infants. Public Library of Science 2016-04-25 /pmc/articles/PMC4844123/ /pubmed/27111847 http://dx.doi.org/10.1371/journal.pone.0152751 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Cong, Xiaomei
Xu, Wanli
Janton, Susan
Henderson, Wendy A.
Matson, Adam
McGrath, Jacqueline M.
Maas, Kendra
Graf, Joerg
Gut Microbiome Developmental Patterns in Early Life of Preterm Infants: Impacts of Feeding and Gender
title Gut Microbiome Developmental Patterns in Early Life of Preterm Infants: Impacts of Feeding and Gender
title_full Gut Microbiome Developmental Patterns in Early Life of Preterm Infants: Impacts of Feeding and Gender
title_fullStr Gut Microbiome Developmental Patterns in Early Life of Preterm Infants: Impacts of Feeding and Gender
title_full_unstemmed Gut Microbiome Developmental Patterns in Early Life of Preterm Infants: Impacts of Feeding and Gender
title_short Gut Microbiome Developmental Patterns in Early Life of Preterm Infants: Impacts of Feeding and Gender
title_sort gut microbiome developmental patterns in early life of preterm infants: impacts of feeding and gender
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4844123/
https://www.ncbi.nlm.nih.gov/pubmed/27111847
http://dx.doi.org/10.1371/journal.pone.0152751
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