Cargando…
Isolation and Characterization of Microsatellite Markers for Mimusops balata (Sapotaceae) and Cross-Amplification in Other Mimusops Species
Mimusops balata (Sapotaceae) is an endemic tree species from La Réunion and Mauritius. Like many species growing in lowland forests in La Réunion, it has suffered from human disturbances. We developed twelve microsatellite markers for M. balata and tested cross-amplification in five other Mimusops s...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4844264/ https://www.ncbi.nlm.nih.gov/pubmed/27137643 http://dx.doi.org/10.3390/plants1020100 |
_version_ | 1782428737406500864 |
---|---|
author | Dafreville, Stéphanie Baider, Cláudia Florens, F. B. Vincent Lebreton, Gérard Rivière, Eric Strasberg, Dominique Chevallier, Marie-Hélène |
author_facet | Dafreville, Stéphanie Baider, Cláudia Florens, F. B. Vincent Lebreton, Gérard Rivière, Eric Strasberg, Dominique Chevallier, Marie-Hélène |
author_sort | Dafreville, Stéphanie |
collection | PubMed |
description | Mimusops balata (Sapotaceae) is an endemic tree species from La Réunion and Mauritius. Like many species growing in lowland forests in La Réunion, it has suffered from human disturbances. We developed twelve microsatellite markers for M. balata and tested cross-amplification in five other Mimusops species to have powerful tools for genetic diversity studies. Genotyping peaks were of very low quality for two loci and were consequently abandoned for the genetic diversity analyses. Ten microsatellite loci were tested on 34 individuals of M. balata from two natural populations. The number of alleles per locus ranged from one to seven. The observed and expected heterozygosity levels varied from 0.000 to 0.823, and from 0.000 to 0.812 respectively. Two loci deviated from the Hardy-Weinberg equilibrium. The presence of null alleles was detected for one of these two loci. Nine to ten loci cross-amplified reliably in Mauritian species, for the other three species, four to six loci show successful amplifications. These polymorphic microsatellite markers are now available for population genetic investigations in Mimusops species aiming to establish accurate guidelines for conservation managers. |
format | Online Article Text |
id | pubmed-4844264 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-48442642016-04-29 Isolation and Characterization of Microsatellite Markers for Mimusops balata (Sapotaceae) and Cross-Amplification in Other Mimusops Species Dafreville, Stéphanie Baider, Cláudia Florens, F. B. Vincent Lebreton, Gérard Rivière, Eric Strasberg, Dominique Chevallier, Marie-Hélène Plants (Basel) Short Note Mimusops balata (Sapotaceae) is an endemic tree species from La Réunion and Mauritius. Like many species growing in lowland forests in La Réunion, it has suffered from human disturbances. We developed twelve microsatellite markers for M. balata and tested cross-amplification in five other Mimusops species to have powerful tools for genetic diversity studies. Genotyping peaks were of very low quality for two loci and were consequently abandoned for the genetic diversity analyses. Ten microsatellite loci were tested on 34 individuals of M. balata from two natural populations. The number of alleles per locus ranged from one to seven. The observed and expected heterozygosity levels varied from 0.000 to 0.823, and from 0.000 to 0.812 respectively. Two loci deviated from the Hardy-Weinberg equilibrium. The presence of null alleles was detected for one of these two loci. Nine to ten loci cross-amplified reliably in Mauritian species, for the other three species, four to six loci show successful amplifications. These polymorphic microsatellite markers are now available for population genetic investigations in Mimusops species aiming to establish accurate guidelines for conservation managers. MDPI 2012-12-17 /pmc/articles/PMC4844264/ /pubmed/27137643 http://dx.doi.org/10.3390/plants1020100 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Short Note Dafreville, Stéphanie Baider, Cláudia Florens, F. B. Vincent Lebreton, Gérard Rivière, Eric Strasberg, Dominique Chevallier, Marie-Hélène Isolation and Characterization of Microsatellite Markers for Mimusops balata (Sapotaceae) and Cross-Amplification in Other Mimusops Species |
title | Isolation and Characterization of Microsatellite Markers for Mimusops balata (Sapotaceae) and Cross-Amplification in Other Mimusops Species |
title_full | Isolation and Characterization of Microsatellite Markers for Mimusops balata (Sapotaceae) and Cross-Amplification in Other Mimusops Species |
title_fullStr | Isolation and Characterization of Microsatellite Markers for Mimusops balata (Sapotaceae) and Cross-Amplification in Other Mimusops Species |
title_full_unstemmed | Isolation and Characterization of Microsatellite Markers for Mimusops balata (Sapotaceae) and Cross-Amplification in Other Mimusops Species |
title_short | Isolation and Characterization of Microsatellite Markers for Mimusops balata (Sapotaceae) and Cross-Amplification in Other Mimusops Species |
title_sort | isolation and characterization of microsatellite markers for mimusops balata (sapotaceae) and cross-amplification in other mimusops species |
topic | Short Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4844264/ https://www.ncbi.nlm.nih.gov/pubmed/27137643 http://dx.doi.org/10.3390/plants1020100 |
work_keys_str_mv | AT dafrevillestephanie isolationandcharacterizationofmicrosatellitemarkersformimusopsbalatasapotaceaeandcrossamplificationinothermimusopsspecies AT baiderclaudia isolationandcharacterizationofmicrosatellitemarkersformimusopsbalatasapotaceaeandcrossamplificationinothermimusopsspecies AT florensfbvincent isolationandcharacterizationofmicrosatellitemarkersformimusopsbalatasapotaceaeandcrossamplificationinothermimusopsspecies AT lebretongerard isolationandcharacterizationofmicrosatellitemarkersformimusopsbalatasapotaceaeandcrossamplificationinothermimusopsspecies AT riviereeric isolationandcharacterizationofmicrosatellitemarkersformimusopsbalatasapotaceaeandcrossamplificationinothermimusopsspecies AT strasbergdominique isolationandcharacterizationofmicrosatellitemarkersformimusopsbalatasapotaceaeandcrossamplificationinothermimusopsspecies AT chevalliermariehelene isolationandcharacterizationofmicrosatellitemarkersformimusopsbalatasapotaceaeandcrossamplificationinothermimusopsspecies |