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Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds
In our previous report, an 80% ethanol bitter gourd seed extract (BGSE) was found to suppress proliferation of adult T-cell leukemia (ATL) cell lines. The present study aimed to identify the bioactive compounds from BGSE specific against ATL. From the result of an HPLC-MS analysis, α-eleostearic aci...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4844309/ https://www.ncbi.nlm.nih.gov/pubmed/27135489 http://dx.doi.org/10.3390/plants3010018 |
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author | Kai, Hisahiro Akamatsu, Ena Torii, Eri Kodama, Hiroko Yukizaki, Chizuko Akagi, Isao Ino, Hisatoshi Sakakibara, Yoichi Suiko, Masahito Yamamoto, Ikuo Okayama, Akihiko Morishita, Kazuhiro Kataoka, Hiroaki Matsuno, Koji |
author_facet | Kai, Hisahiro Akamatsu, Ena Torii, Eri Kodama, Hiroko Yukizaki, Chizuko Akagi, Isao Ino, Hisatoshi Sakakibara, Yoichi Suiko, Masahito Yamamoto, Ikuo Okayama, Akihiko Morishita, Kazuhiro Kataoka, Hiroaki Matsuno, Koji |
author_sort | Kai, Hisahiro |
collection | PubMed |
description | In our previous report, an 80% ethanol bitter gourd seed extract (BGSE) was found to suppress proliferation of adult T-cell leukemia (ATL) cell lines. The present study aimed to identify the bioactive compounds from BGSE specific against ATL. From the result of an HPLC-MS analysis, α-eleostearic acid (α-ESA) was present in BGSE at 0.68% ± 0.0022% (±SD, n = 5). In the cell proliferation test, α-ESA potently suppressed proliferation of two ATL cell lines (ED and Su9T01; IC(50) = 8.9 and 29.3 µM, respectively) more than several other octadecanoic acids. However, α-ESA moderately inhibited phytohemagglutinin-activated human peripheral blood mononuclear cells (PBMC; IC(50) = 31.0 µM). These results suggest that BGSE-derived α-ESA has potential as a functional food constituent because of its activity against ATL, particularly against ED cells. Moreover, α-ESA might be effective for the prevention of moderate adverse effects of ATL on normal T cells. |
format | Online Article Text |
id | pubmed-4844309 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-48443092016-04-29 Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds Kai, Hisahiro Akamatsu, Ena Torii, Eri Kodama, Hiroko Yukizaki, Chizuko Akagi, Isao Ino, Hisatoshi Sakakibara, Yoichi Suiko, Masahito Yamamoto, Ikuo Okayama, Akihiko Morishita, Kazuhiro Kataoka, Hiroaki Matsuno, Koji Plants (Basel) Article In our previous report, an 80% ethanol bitter gourd seed extract (BGSE) was found to suppress proliferation of adult T-cell leukemia (ATL) cell lines. The present study aimed to identify the bioactive compounds from BGSE specific against ATL. From the result of an HPLC-MS analysis, α-eleostearic acid (α-ESA) was present in BGSE at 0.68% ± 0.0022% (±SD, n = 5). In the cell proliferation test, α-ESA potently suppressed proliferation of two ATL cell lines (ED and Su9T01; IC(50) = 8.9 and 29.3 µM, respectively) more than several other octadecanoic acids. However, α-ESA moderately inhibited phytohemagglutinin-activated human peripheral blood mononuclear cells (PBMC; IC(50) = 31.0 µM). These results suggest that BGSE-derived α-ESA has potential as a functional food constituent because of its activity against ATL, particularly against ED cells. Moreover, α-ESA might be effective for the prevention of moderate adverse effects of ATL on normal T cells. MDPI 2013-12-27 /pmc/articles/PMC4844309/ /pubmed/27135489 http://dx.doi.org/10.3390/plants3010018 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Kai, Hisahiro Akamatsu, Ena Torii, Eri Kodama, Hiroko Yukizaki, Chizuko Akagi, Isao Ino, Hisatoshi Sakakibara, Yoichi Suiko, Masahito Yamamoto, Ikuo Okayama, Akihiko Morishita, Kazuhiro Kataoka, Hiroaki Matsuno, Koji Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds |
title | Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds |
title_full | Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds |
title_fullStr | Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds |
title_full_unstemmed | Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds |
title_short | Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds |
title_sort | identification of a bioactive compound against adult t-cell leukaemia from bitter gourd seeds |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4844309/ https://www.ncbi.nlm.nih.gov/pubmed/27135489 http://dx.doi.org/10.3390/plants3010018 |
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