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Hormonal Regulation and Expression Profiles of Wheat Genes Involved during Phytic Acid Biosynthesis Pathway

Phytic acid (PA) biosynthesis pathway genes were reported from multiple crop species. PA accumulation was enhanced during grain filling and at that time, hormones like Abscisic acid (ABA) and Gibberellic acid (GA(3)) interplay to control the process of seed development. Regulation of wheat PA pathwa...

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Autores principales: Aggarwal, Sipla, Shukla, Vishnu, Bhati, Kaushal Kumar, Kaur, Mandeep, Sharma, Shivani, Singh, Anuradha, Mantri, Shrikant, Pandey, Ajay Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4844322/
https://www.ncbi.nlm.nih.gov/pubmed/27135330
http://dx.doi.org/10.3390/plants4020298
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author Aggarwal, Sipla
Shukla, Vishnu
Bhati, Kaushal Kumar
Kaur, Mandeep
Sharma, Shivani
Singh, Anuradha
Mantri, Shrikant
Pandey, Ajay Kumar
author_facet Aggarwal, Sipla
Shukla, Vishnu
Bhati, Kaushal Kumar
Kaur, Mandeep
Sharma, Shivani
Singh, Anuradha
Mantri, Shrikant
Pandey, Ajay Kumar
author_sort Aggarwal, Sipla
collection PubMed
description Phytic acid (PA) biosynthesis pathway genes were reported from multiple crop species. PA accumulation was enhanced during grain filling and at that time, hormones like Abscisic acid (ABA) and Gibberellic acid (GA(3)) interplay to control the process of seed development. Regulation of wheat PA pathway genes has not yet been reported in seeds. In an attempt to find the clues for the regulation by hormones, the promoter region of wheat PA pathway genes was analyzed for the presence of cis-elements. Multiple cis-elements of those known to be involved for ABA, GA(3), salicylic acid (SA), and cAMP sensing were identified in the promoters of PA pathway genes. Eight genes (TaIMP, TaITPK1-4, TaPLC1, TaIPK2 and TaIPK1) involved in the wheat PA biosynthesis pathway were selected for the expression studies. The temporal expression response was studied in seeds treated with ABA and GA(3) using quantitative real time PCR. Our results suggested that exogenous application of ABA induces few PA pathway genes in wheat grains. Comparison of expression profiles for PA pathway for GA(3) and ABA suggested the antagonistic regulation of certain genes. Additionally, to reveal stress responses of wheat PA pathway genes, expression was also studied in the presence of SA and cAMP. Results suggested SA specific differential expression of few genes, whereas, overall repression of genes was observed in cAMP treated samples. This study is an effort to understand the regulation of PA biosynthesis genes in wheat.
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spelling pubmed-48443222016-04-29 Hormonal Regulation and Expression Profiles of Wheat Genes Involved during Phytic Acid Biosynthesis Pathway Aggarwal, Sipla Shukla, Vishnu Bhati, Kaushal Kumar Kaur, Mandeep Sharma, Shivani Singh, Anuradha Mantri, Shrikant Pandey, Ajay Kumar Plants (Basel) Article Phytic acid (PA) biosynthesis pathway genes were reported from multiple crop species. PA accumulation was enhanced during grain filling and at that time, hormones like Abscisic acid (ABA) and Gibberellic acid (GA(3)) interplay to control the process of seed development. Regulation of wheat PA pathway genes has not yet been reported in seeds. In an attempt to find the clues for the regulation by hormones, the promoter region of wheat PA pathway genes was analyzed for the presence of cis-elements. Multiple cis-elements of those known to be involved for ABA, GA(3), salicylic acid (SA), and cAMP sensing were identified in the promoters of PA pathway genes. Eight genes (TaIMP, TaITPK1-4, TaPLC1, TaIPK2 and TaIPK1) involved in the wheat PA biosynthesis pathway were selected for the expression studies. The temporal expression response was studied in seeds treated with ABA and GA(3) using quantitative real time PCR. Our results suggested that exogenous application of ABA induces few PA pathway genes in wheat grains. Comparison of expression profiles for PA pathway for GA(3) and ABA suggested the antagonistic regulation of certain genes. Additionally, to reveal stress responses of wheat PA pathway genes, expression was also studied in the presence of SA and cAMP. Results suggested SA specific differential expression of few genes, whereas, overall repression of genes was observed in cAMP treated samples. This study is an effort to understand the regulation of PA biosynthesis genes in wheat. MDPI 2015-06-11 /pmc/articles/PMC4844322/ /pubmed/27135330 http://dx.doi.org/10.3390/plants4020298 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aggarwal, Sipla
Shukla, Vishnu
Bhati, Kaushal Kumar
Kaur, Mandeep
Sharma, Shivani
Singh, Anuradha
Mantri, Shrikant
Pandey, Ajay Kumar
Hormonal Regulation and Expression Profiles of Wheat Genes Involved during Phytic Acid Biosynthesis Pathway
title Hormonal Regulation and Expression Profiles of Wheat Genes Involved during Phytic Acid Biosynthesis Pathway
title_full Hormonal Regulation and Expression Profiles of Wheat Genes Involved during Phytic Acid Biosynthesis Pathway
title_fullStr Hormonal Regulation and Expression Profiles of Wheat Genes Involved during Phytic Acid Biosynthesis Pathway
title_full_unstemmed Hormonal Regulation and Expression Profiles of Wheat Genes Involved during Phytic Acid Biosynthesis Pathway
title_short Hormonal Regulation and Expression Profiles of Wheat Genes Involved during Phytic Acid Biosynthesis Pathway
title_sort hormonal regulation and expression profiles of wheat genes involved during phytic acid biosynthesis pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4844322/
https://www.ncbi.nlm.nih.gov/pubmed/27135330
http://dx.doi.org/10.3390/plants4020298
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