Cargando…
Growth Media Induces Variation in Cell Wall Associated Gene Expression in Arabidopsis thaliana Pollen Tube
The influence of three different pollen germination media on the transcript profile of Arabidopsis pollen tubes has been assessed by real-time PCR on a selection of cell wall related genes, and by a statistical analysis of microarray Arabidopsis pollen tube data sets. The qPCR assays have shown rema...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4844376/ https://www.ncbi.nlm.nih.gov/pubmed/27137385 http://dx.doi.org/10.3390/plants2030429 |
_version_ | 1782428759202201600 |
---|---|
author | da Costa, Mário Luís Pereira, Luís Gustavo Coimbra, Sílvia |
author_facet | da Costa, Mário Luís Pereira, Luís Gustavo Coimbra, Sílvia |
author_sort | da Costa, Mário Luís |
collection | PubMed |
description | The influence of three different pollen germination media on the transcript profile of Arabidopsis pollen tubes has been assessed by real-time PCR on a selection of cell wall related genes, and by a statistical analysis of microarray Arabidopsis pollen tube data sets. The qPCR assays have shown remarkable differences on the transcript levels of specific genes depending upon the formulation of the germination medium used. With the aid of principal component analysis performed on existing microarray data, a subset of genes has been identified that is more prone to produce diverging transcript levels. A functional classification of those genes showed that the clusters with higher number of members were those for hydrolase activity (based in molecular function) and for cell wall (based in cellular component). Taken together, these results may indicate that the nutrient composition of the pollen germination media influences pollen tube metabolism and that caution must be taken when interpreting transcriptomic data of pollen tubes. |
format | Online Article Text |
id | pubmed-4844376 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-48443762016-04-29 Growth Media Induces Variation in Cell Wall Associated Gene Expression in Arabidopsis thaliana Pollen Tube da Costa, Mário Luís Pereira, Luís Gustavo Coimbra, Sílvia Plants (Basel) Article The influence of three different pollen germination media on the transcript profile of Arabidopsis pollen tubes has been assessed by real-time PCR on a selection of cell wall related genes, and by a statistical analysis of microarray Arabidopsis pollen tube data sets. The qPCR assays have shown remarkable differences on the transcript levels of specific genes depending upon the formulation of the germination medium used. With the aid of principal component analysis performed on existing microarray data, a subset of genes has been identified that is more prone to produce diverging transcript levels. A functional classification of those genes showed that the clusters with higher number of members were those for hydrolase activity (based in molecular function) and for cell wall (based in cellular component). Taken together, these results may indicate that the nutrient composition of the pollen germination media influences pollen tube metabolism and that caution must be taken when interpreting transcriptomic data of pollen tubes. MDPI 2013-06-25 /pmc/articles/PMC4844376/ /pubmed/27137385 http://dx.doi.org/10.3390/plants2030429 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article da Costa, Mário Luís Pereira, Luís Gustavo Coimbra, Sílvia Growth Media Induces Variation in Cell Wall Associated Gene Expression in Arabidopsis thaliana Pollen Tube |
title | Growth Media Induces Variation in Cell Wall Associated Gene Expression in Arabidopsis thaliana Pollen Tube |
title_full | Growth Media Induces Variation in Cell Wall Associated Gene Expression in Arabidopsis thaliana Pollen Tube |
title_fullStr | Growth Media Induces Variation in Cell Wall Associated Gene Expression in Arabidopsis thaliana Pollen Tube |
title_full_unstemmed | Growth Media Induces Variation in Cell Wall Associated Gene Expression in Arabidopsis thaliana Pollen Tube |
title_short | Growth Media Induces Variation in Cell Wall Associated Gene Expression in Arabidopsis thaliana Pollen Tube |
title_sort | growth media induces variation in cell wall associated gene expression in arabidopsis thaliana pollen tube |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4844376/ https://www.ncbi.nlm.nih.gov/pubmed/27137385 http://dx.doi.org/10.3390/plants2030429 |
work_keys_str_mv | AT dacostamarioluis growthmediainducesvariationincellwallassociatedgeneexpressioninarabidopsisthalianapollentube AT pereiraluisgustavo growthmediainducesvariationincellwallassociatedgeneexpressioninarabidopsisthalianapollentube AT coimbrasilvia growthmediainducesvariationincellwallassociatedgeneexpressioninarabidopsisthalianapollentube |