Cargando…

SPL8 Acts Together with the Brassinosteroid-Signaling Component BIM1 in Controlling Arabidopsis thaliana Male Fertility

The non-miR156 targeted SBP-box gene SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 8 (SPL8), plays an important role in Arabidopsis anther development, where its loss-of-function results in a semi-sterile phenotype. Fully male-sterile plants are obtained when a spl8 loss-of-function mutation is introduced...

Descripción completa

Detalles Bibliográficos
Autores principales: Xing, Shuping, Quodt, Vanessa, Chandler, John, Höhmann, Susanne, Berndtgen, Rita, Huijser, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4844382/
https://www.ncbi.nlm.nih.gov/pubmed/27137384
http://dx.doi.org/10.3390/plants2030416
_version_ 1782428760538087424
author Xing, Shuping
Quodt, Vanessa
Chandler, John
Höhmann, Susanne
Berndtgen, Rita
Huijser, Peter
author_facet Xing, Shuping
Quodt, Vanessa
Chandler, John
Höhmann, Susanne
Berndtgen, Rita
Huijser, Peter
author_sort Xing, Shuping
collection PubMed
description The non-miR156 targeted SBP-box gene SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 8 (SPL8), plays an important role in Arabidopsis anther development, where its loss-of-function results in a semi-sterile phenotype. Fully male-sterile plants are obtained when a spl8 loss-of-function mutation is introduced into a 35S:MIR156 genetic background, thereby revealing functional redundancy between SPL8 and miR156-targeted SBP-box genes. Here, we show that BIM1, a gene encoding a bHLH protein involved in brassinosteroid signaling and embryonic patterning, functions redundantly with SPL8 in its requirement for male fertility. Although bim1 single mutants displayed a mild fertility problem due to shortened filaments in some flowers, mutation of BIM1 significantly enhanced the semi-sterile phenotype of the spl8 mutant. Expression of both SPL8 and BIM1 was detected in overlapping expression domains during early anther developmental stages. Our data suggest that in regulating anther development, SPL8 and BIM1 function cooperatively in a common complex or in synergistic pathways. Phylogenetic analysis supports the idea of an evolutionary conserved function for both genes in angiosperm anther development.
format Online
Article
Text
id pubmed-4844382
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-48443822016-04-29 SPL8 Acts Together with the Brassinosteroid-Signaling Component BIM1 in Controlling Arabidopsis thaliana Male Fertility Xing, Shuping Quodt, Vanessa Chandler, John Höhmann, Susanne Berndtgen, Rita Huijser, Peter Plants (Basel) Article The non-miR156 targeted SBP-box gene SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 8 (SPL8), plays an important role in Arabidopsis anther development, where its loss-of-function results in a semi-sterile phenotype. Fully male-sterile plants are obtained when a spl8 loss-of-function mutation is introduced into a 35S:MIR156 genetic background, thereby revealing functional redundancy between SPL8 and miR156-targeted SBP-box genes. Here, we show that BIM1, a gene encoding a bHLH protein involved in brassinosteroid signaling and embryonic patterning, functions redundantly with SPL8 in its requirement for male fertility. Although bim1 single mutants displayed a mild fertility problem due to shortened filaments in some flowers, mutation of BIM1 significantly enhanced the semi-sterile phenotype of the spl8 mutant. Expression of both SPL8 and BIM1 was detected in overlapping expression domains during early anther developmental stages. Our data suggest that in regulating anther development, SPL8 and BIM1 function cooperatively in a common complex or in synergistic pathways. Phylogenetic analysis supports the idea of an evolutionary conserved function for both genes in angiosperm anther development. MDPI 2013-06-25 /pmc/articles/PMC4844382/ /pubmed/27137384 http://dx.doi.org/10.3390/plants2030416 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Xing, Shuping
Quodt, Vanessa
Chandler, John
Höhmann, Susanne
Berndtgen, Rita
Huijser, Peter
SPL8 Acts Together with the Brassinosteroid-Signaling Component BIM1 in Controlling Arabidopsis thaliana Male Fertility
title SPL8 Acts Together with the Brassinosteroid-Signaling Component BIM1 in Controlling Arabidopsis thaliana Male Fertility
title_full SPL8 Acts Together with the Brassinosteroid-Signaling Component BIM1 in Controlling Arabidopsis thaliana Male Fertility
title_fullStr SPL8 Acts Together with the Brassinosteroid-Signaling Component BIM1 in Controlling Arabidopsis thaliana Male Fertility
title_full_unstemmed SPL8 Acts Together with the Brassinosteroid-Signaling Component BIM1 in Controlling Arabidopsis thaliana Male Fertility
title_short SPL8 Acts Together with the Brassinosteroid-Signaling Component BIM1 in Controlling Arabidopsis thaliana Male Fertility
title_sort spl8 acts together with the brassinosteroid-signaling component bim1 in controlling arabidopsis thaliana male fertility
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4844382/
https://www.ncbi.nlm.nih.gov/pubmed/27137384
http://dx.doi.org/10.3390/plants2030416
work_keys_str_mv AT xingshuping spl8actstogetherwiththebrassinosteroidsignalingcomponentbim1incontrollingarabidopsisthalianamalefertility
AT quodtvanessa spl8actstogetherwiththebrassinosteroidsignalingcomponentbim1incontrollingarabidopsisthalianamalefertility
AT chandlerjohn spl8actstogetherwiththebrassinosteroidsignalingcomponentbim1incontrollingarabidopsisthalianamalefertility
AT hohmannsusanne spl8actstogetherwiththebrassinosteroidsignalingcomponentbim1incontrollingarabidopsisthalianamalefertility
AT berndtgenrita spl8actstogetherwiththebrassinosteroidsignalingcomponentbim1incontrollingarabidopsisthalianamalefertility
AT huijserpeter spl8actstogetherwiththebrassinosteroidsignalingcomponentbim1incontrollingarabidopsisthalianamalefertility